Techniques for the generation of transgenic mice - theory and practical exercises
EMBL Heidelberg Tuesday 21 September - Wednesday 22 September 2010
Programme
Preliminary Programme
| Tuesday, 21 September 2010 |
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| Introduction to generation of transgenic mice, microinjection and correct microscope usage and contrast techniques. |
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Practical 1: Correct microscope usage and contrast techniques Practical 2: Microinjection into pronuclei |
| Wednesday, 22 September 2010 |
| Production of transgenic mice, laser-assisted ES transfer |
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Practical 3: Transfer of embryonic stem cells into mouse blastocysts Practical 4: Laser-assisted ES transfer into 8-cell embryo Troubleshooting |