Microinjection into adherent cells- theory and practical exercises
EMBL Heidelberg Wednesday 24 November - Thursday 25 November 2010
Programme
Preliminary Programme
Wednesday, 24 November 2010
Introduction to microinjection and fundamentals of light- and fluorescence microscopy.
Practical 1: Correct usage of microscope and contrast techniques
Practical 2: Sample preparation and injection of fluorescent dyes
Thursday, 25 November 2010
Microinjection and accurate Microscopy analysis
Practical 3: Injection of different GFP vectors
Practical 4: Results analysis
Troubleshooting