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This course is aimed at young scientists in life sciences at the pre- or post-doctoral level as well as young investigators with a strong interest to learn more on advanced live cell imaging methods and applying them to their own research. About 40% of the course program covers lectures and tutorials on basic and advanced microscopy methods and applications by experts in the field. The rest of the time will focus on hands on practical work organised in small groups. These practicals will address the following topics: Basic aspects of fluorescence microscopy, characterisation of state of the art imaging detectors, multicolour 3D time-lapse microscopy of living samples, total internal reflection fluorescence microscopy, fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer measurements (FRET), imaging of thick samples such as embryos or tissue sections, basic and advanced image analysis. Participants are encouraged to bring along their own samples as they will have the possibility to image their own samples and discuss results with the course teachers. A poster session will be organized where all participants will have the opportunity to present and discuss their own scientific work.