Targeted Genome Editing using Zinc Finger Nucleases
EMBL Heidelberg Tuesday 22 June - Thursday 24 June 2010
Programme
|
Tuesday 22 June 2010 |
|
|---|---|
| 09.00 | Welcome |
| Introduction to ZFN Technology | |
| ZFN Design | |
| Genome Editing and Delivery | |
| Nucleofection Overview | |
| Lab Practical: Nucleofection | |
| Double-Strand Break (DSB) Repair | |
| Gene Knockout and Validation | |
| Wine & Cheese in the Rooftop Lounge | |
| 20.00 | Bus to hotel |
|
Wednesday 23 June 2010 |
|
| 09.00 | Gene Insertion and Tagging |
| Donor Plasmid Design | |
| Lab Practical: Cel-1 Assay | |
| Integration Detection | |
| Dilution Cloning and Genotyping | |
| Quantitation of ZFN Cel-1 Clevage Activity | |
| Lab Practical: Junction PCR | |
| Lab Practical: RFLP Assay | |
| 18.30 | Bus to hotel |
|
Thursday 24 June 2010 |
|
| 09.00 | Lab Practical: Junction PCR |
| Lab Practical: RFLP Assay | |
| Guest Lecture | |
| Troubleshooting Exercise | |
| Lab Practical: Results | |
| Transgenics | |
| Therapeutics | |
| 17.00 | End of course & bus to train station |