EMBL Advanced Course
Proteome Analysis by Mass Spectrometry - From Samples to Data Analysis
EMBL Heidelberg, Germany Monday 4 July - Friday 8 July 2011
Programme
| Monday, 4 July: Introduction to Proteomics | |
|---|---|
| 09:00 | Welcome and Introduction to Proteomics Protein interactions and the capture of protein complexes Separation Technologies |
| Lunch Break | |
| Practical session- protein purification | |
| 19:00 | Dinner Wine and cheese reception at EMBL |
| Tuesday, 5 July: Mass spectrometry | |
| 09:00 | Practical Session- protein separarion |
| MS instrumentation 1: ionization and mass analysis | |
| MS instrumentation 2: LCMS & protein identification by MS | |
| Lunch | |
| Practical session- sample preparation | |
| 18:30 | Dinner |
| Wednesday, 6 July: Mass spectrometry | |
| 09:00 | Peptide Fragmentation Manual interpretation of MSMS spectra MS Search Engines |
| Lunch | |
| Practical Session- MALDI, LC-ion trap, Orbitrap | |
| 19:00 | Free evening |
| Thursday, 7 July: Data Processing and Analysis | |
| 09:00 | Phosphoproteomics and PTM detection Quantitative proteomics Targeted proteomics and MRM: instruments and applications, experimental design |
| Lunch | |
| Practical Session- MS practical and interpretation of MS results | |
| 19:00 | Dinner in Heidelberg Downtown |
| Friday, 8 July: Data Mining | |
| 09:00 | Data mining: Biocompendium (intro and practical) Protein interaction and pathway databases Cytoscape (Intro and practical) |
| Lunch | |
| Doctor’s office: questions from participants’ practice | |
| Closing discussion | |
| 17:00 | End of course |