Next Generation Sequencing Data Analysis

Talk: Vladimir Benes, GeneCore at EMBL Heidelberg, on NGS library preparation

• Introduction to Genomatix Mining Station (GMS) - methodological background
• GMS demonstration: mapping, SNP detection, read classification
• Hands-on example using the GGA: SNP analysis and evaluation

Talk: Jonathon Blake, GeneCore at EMBL Heidelberg, on RNA sequencing

Hands-on examples using the GGA (expression analysis):

• Differential expression
• Assessment of related biology
• Data visualization

Hands-on examples using the GGA (ChIP-Seq analysis):

• Expression analysis
• Data visualization
• Peak detection and classification
• TF binding site analysis in ChIP peaks
• De novo definition of common sequence motifs from ChIP data
• Next-neighbor analysis and regulatory target prediction for ChIP regions
• Meta analysis of different data sets