EMBO Practical Course
High Throughput Microscopy for Systems BiologyEMBL Heidelberg, Germany Monday 15 October - Saturday 20 October 2012 Application closed
The application process is now closed. Results will be sent to the applicants in early August.
Why You Should Apply
The information in complete genome sequences provide the challenging opportunity to analyse the complexity of biological processes on a large scale, with the goal of reaching a more complete and quantitative description of their molecular regulation. High-throughput techniques such as protein analysis by mass spectrometry, or expression and transcription profiling by protein or DNA microarrays or high throughput DNA, RNA sequencing are successfully applied to address this challenge in diverse biological questions.
However, despite their great usefulness, these techniques cannot provide adequate temporal or spatial resolution at the cellular or organism level. Fluorescence-based imaging assays in intact living cells overcome these limitations, because they can probe the function of macromolecules in their natural environment inside living cells with exquisite and ever increasing spatial and temporal resolution. Recent technical advances in reagents that interfere with cellular functions or visualize cellular molecules, high throughput transfection methods and fluorescence microscopy allow now large-scale fluorescence imaging of intact cells in combination with genome-wide RNA interference (RNAi), overexpression of cDNAs or small-molecule-based perturbations, or libraries of fluorescently tagged proteins.
This course will teach all aspects of high throughput fluorescence microscopy including assay development, large-scale transfection methods, automated image acquisition, data handling and analysis, statistical analyses and data mining, as well as image databases.
Presentations on applications of high throughput microscopy to diverse biological problems will be given by leaders in the field. Industrial partners will present their latest developments in hands-on sessions.
- siRNA design and siRNA library bioinformatics
- high-throughput (HT) transfections (reverse transfection, microtiter plates, siRNA microarrays)
- assay development of robust live cell based fluorescence assays for HT microscopy
- design and implementation of image processing to quantify the assay
- designing and performing a pilot screen with appropriate controls
- critical evaluation of pilot screen performance and adaptation of cell based assay
- automated microscopy data acquisition comparing different imaging systems
- image analysis, data processing of large scale data sets
- quality control and statistical analysis
- data mining, modeling and interpretation of screening results
- dissemination of screening results via databases
Who Should Apply
This course aims predominantly at young scientists at the pre-doc and early post-doctoral level who wish to apply high throughput microscopy to their own projects.
After the course
For a limited number of course students, it will be possible to access EMBL’s High Throughput Screening Microscopy Facility after the course to carry out focused project work building on and applying the training they received.