EMBO Practical Course
High Throughput Microscopy for Systems Biology
EMBL Heidelberg, Germany Monday 15 October - Saturday 20 October 2012 Application closed
(Preliminary) Programme
Practical work
Monday and Tuesday P1-P4
P1: Coating multiwall plates ready for reverse transfection. H. Erfle and F. Neumann
P2: Sample preparation. J. Bulkescher and B. Koch
P3: Assay design: C. Conrad, J. Ellenberg, R. Pepperkok and J. Simpson
P4: Concepts and tools for quality control. B. Fischer, S. Sauer, Ch. Tischer
Wednesday and Thursday P5-P8
P5: Time-lapse analysis of chromosome condensation. J-K. Heriche and Julia Roberti. F. Neumann and Matthias Rommeswinkel ( Olympus)
P6: Drug treatment induced Golgi dynamics. C. Conrad and R. Pepperkok
P7: Inner nuclear membrane targeting. A. Boni and Ch. Tischer
P8: Microtubuli secondary screening. J. Bulkescher and M. Isokane
Friday and Saturday P9-P12
P9: Image analysis of time-lapse data. Ch. Sommer and T. Walter
P10: Image analysis of P6 using Cell Profiler. Ch. Tischer and R. Pepperkok, Zvi Kam
P11: Image analysis of P7. A. Boni and K. Miura
P12: Large scale analysis and modeling. B. Fischer, J.-K. Heriche, W. Huber
Day 1 - Monday, 15 October 2012
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09:00 - 10:00 |
Lecture by Jan Ellenberg “High throughput microscopy: From RNAi screening to in vivo proteomics” |
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10:00 – 11:00 |
Lecture by Wolfgang Huber “Quality control and statistical analysis” |
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11:00 - 11:40 11:40 – 12:00 |
Tutorial1: Beate Neumann “siRNA libraries” Tutorial 2: Sajoscha Sauer “Introduction LIMS” |
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12:00 - 13:00 |
Lunch (EMBL Canteen) |
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13:00 - 19:00 |
Practical workshops (P1-P4) – see outline above |
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19:00 – 20:00 |
Dinner (EMBL Canteen) |
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20:00- 22:00 |
Poster session with wine and cheese (Top of Helix A and ATC Lounge) |
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22:00 |
Bus transfer ISG Hotel |
Day 2 - Tuesday, 16 October 2012
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09:00 - 10:00 |
Lecture by Jez Simpson “High content analysis strategies to dissect early secretory pathway function” |
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10:00 - 11:00 |
Lecture by Paul French - “Fluorescence lifetime imaging for High Content Analysis” |
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11:00 - 12:00 |
Lecture by Olli Kallioniemi – “High-throughput approaches for individualized systems medicine” |
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12:00 - 13:00 |
Lunch (EMBL Canteen) |
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13:00 - 19:00 |
Practical workshops (P1-P4) – see outline below |
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19:00 – 20:00 |
Dinner (EMBL Canteen) |
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20:00 - 22:00 |
Tutorial 2 : Kota Miura “Image J 1, J 2” |
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22:10 |
Bus transfer ISG Hotel |
Day 3 - Wednesday, 17 October 2012
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09:00 - 12:00 |
Demonstration of HTM equipment by companies: Olympus Room ALMF / 508B "HT Acquisition and Analysis with Olympus Scan^R" GE HealthCare Room ATC / 116B PerkinElmer Room ATC / 117A “Operetta and Harmony – High Throughput Biology for Everyone” Molecular Devices Room ATC / 116A “The new ImageXpress® Micro XL from Molecular Devices - Your complete Live-Cell Imaging Solution for High Content RNAi Screens” |
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12:00 - 13:00 |
Lunch (EMBL Canteen) |
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13:00 - 19:00 |
Practical workshops (P5-P8) – see outline above |
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19:00 – 20:00 |
Dinner (EMBL Canteen) |
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20:00 - 21:00 |
Lecture by Zvi Kam – “Quantitative Microscope Image Understanding. Low and High throughput analysis” |
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21:10 |
Bus transfer ISG Hotel |
Day 4 - Thursday, 18 October 2012
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09:00 - 10:00 |
Lecture by Daniel Gehrlich “Supervised and unsupervised machine learning for image-based screening” |
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10:00-11:00 |
Lecture by Seamus Holden “Towards high-throughput super-resolution microscopy in bacteria” |
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11:00 - 12:00 |
Lecture by Thomas Walter -"From images to gene clusters: computational approaches for phenotypic profiling" |
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12:00 - 13:00 |
Lunch (EMBL Canteen) |
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13:00 - 19:00 |
Practical workshops (P5-P8) – see outline below |
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19:00 – 20:00 |
Dinner (EMBL Canteen) |
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20:00 |
Bus transfer to ISG or downtown Heidelberg |
Day 5 - Friday, 19 October 2012
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09:00 - 10:00 |
Lecture by Claudia Lukas “Exploring the mammalian DNA damage response using high content siRNA screens: three short stories based on one lucky readout” |
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10:00 -11:00 |
Lecture by Michael Boutros "Mapping of genetic interaction networks by RNAi and high-throughput imaging" |
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11:00 - 12:00 |
Lecture by Malte Wachsmuth "High throughput FCS: parallelization and automation" |
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12:00 - 13:00 |
Lunch (EMBL Canteen) |
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13:00 - 19:00 |
Practical workshops (P9-P12) – see outline above |
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19.00 - 20.00 |
Bus transfer via ISG to downtown Heidelberg |
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20:00 |
Dinner downtown Heidelberg (Restaurant Zum Nepomuk) |
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Individual transport to ISG Hotel |
Day 6 - Saturday, 20 October 2012
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09:00 - 10:00 |
Lecture by Rainer Pepperkok “High throughput microscopy approaches to disease mechanism” |
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10:00 - 13:00 |
Practical workshops (P9-P12) – see outline above |
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13:00 - 14:00 |
Lunch (EMBL Canteen) |
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14:00 - 17:00 |
Practical workshops (P9-P12) – see outline above |
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17:00 -18:00 |
Summary of the course and discussion |
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18:10 |
Bus transfer to ISG Hotel |
