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EMBO Practical Course

High Throughput Microscopy for Systems Biology

EMBL Heidelberg, Germany Monday 15 October - Saturday 20 October 2012 Application closed
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(Preliminary) Programme

The programme is available for download here.

 

Practical work
Monday and Tuesday P1-P4
P1: Coating multiwall plates ready for reverse transfection. H. Erfle and F. Neumann
P2: Sample preparation. J. Bulkescher and B. Koch
P3: Assay design: C. Conrad, J. Ellenberg, R. Pepperkok and J. Simpson
P4: Concepts and tools for quality control. B. Fischer, S. Sauer, Ch. Tischer

Wednesday and Thursday P5-P8
P5: Time-lapse analysis of chromosome condensation. J-K. Heriche and Julia Roberti. F. Neumann and Matthias Rommeswinkel ( Olympus)
P6: Drug treatment induced Golgi dynamics. C. Conrad and R. Pepperkok
P7: Inner nuclear membrane targeting. A. Boni and Ch. Tischer
P8: Microtubuli secondary screening. J. Bulkescher and M. Isokane

Friday and Saturday P9-P12
P9: Image analysis of time-lapse data. Ch. Sommer and T. Walter
P10: Image analysis of P6 using Cell Profiler. Ch. Tischer and R. Pepperkok, Zvi Kam
P11: Image analysis of P7. A. Boni and K. Miura
P12: Large scale analysis and modeling. B. Fischer, J.-K. Heriche, W. Huber

 

Day 1 - Monday, 15 October 2012

09:00 - 10:00

Lecture by Jan Ellenberg “High throughput microscopy: From RNAi screening to in vivo proteomics”

10:00 – 11:00

Lecture by Wolfgang Huber “Quality control and statistical analysis”

11:00 - 11:40

11:40 – 12:00

Tutorial1: Beate Neumann “siRNA libraries” 

Tutorial 2: Sajoscha Sauer “Introduction LIMS”

12:00 - 13:00

Lunch (EMBL Canteen)

13:00 - 19:00

Practical workshops (P1-P4) – see outline above

19:00 – 20:00

Dinner (EMBL Canteen)

20:00- 22:00

Poster session with wine and cheese (Top of Helix A and ATC Lounge)

22:00

Bus transfer ISG Hotel

Day 2 - Tuesday, 16 October 2012

09:00 - 10:00

Lecture by Jez Simpson “High content analysis strategies to dissect early secretory pathway function”

10:00 - 11:00

Lecture by Paul French - “Fluorescence lifetime imaging for High Content Analysis”

11:00 - 12:00

Lecture by Olli Kallioniemi – “High-throughput approaches for individualized systems medicine”

12:00 - 13:00

Lunch (EMBL Canteen)

13:00 - 19:00

Practical workshops (P1-P4) – see outline below 

19:00 – 20:00

Dinner (EMBL Canteen)

20:00 - 22:00

Tutorial 2 : Kota Miura “Image J 1, J 2”

22:10

Bus transfer ISG Hotel

Day 3 - Wednesday, 17 October 2012

09:00 - 12:00

Demonstration of HTM equipment by companies:

Olympus                  Room ALMF / 508B

"HT  Acquisition and Analysis with Olympus Scan^R"

GE HealthCare        Room ATC / 116B

PerkinElmer             Room ATC / 117A

“Operetta and Harmony – High Throughput Biology for Everyone”

Molecular Devices   Room ATC / 116A

“The new ImageXpress® Micro XL from Molecular Devices - Your complete Live-Cell Imaging Solution for High Content RNAi Screens”

12:00 - 13:00

Lunch (EMBL Canteen)

13:00 - 19:00

Practical workshops (P5-P8)  – see outline above

19:00 – 20:00

Dinner (EMBL Canteen)

20:00 - 21:00

Lecture by Zvi Kam – “Quantitative Microscope Image Understanding. Low and High throughput analysis”

21:10

Bus transfer ISG Hotel

Day 4 - Thursday, 18 October 2012

09:00 - 10:00

Lecture by Daniel Gehrlich “Supervised and unsupervised machine learning for image-based screening”

10:00-11:00

Lecture by Seamus Holden “Towards high-throughput super-resolution microscopy in bacteria”

11:00 - 12:00

Lecture by Thomas Walter -"From images to gene clusters: computational approaches for phenotypic profiling"

12:00 - 13:00

Lunch (EMBL Canteen)

13:00 - 19:00

Practical workshops (P5-P8)  – see outline below

19:00 – 20:00

Dinner (EMBL Canteen)

20:00

Bus transfer to ISG or downtown Heidelberg

Day 5 - Friday, 19 October 2012

09:00 - 10:00

Lecture by Claudia Lukas “Exploring the mammalian DNA damage response using high content siRNA screens: three short stories based on one lucky readout”

10:00 -11:00

Lecture by Michael Boutros "Mapping of genetic interaction networks by RNAi and high-throughput imaging"

11:00 - 12:00

Lecture by Malte Wachsmuth "High throughput FCS: parallelization and automation"

12:00 - 13:00

Lunch (EMBL Canteen)

13:00 - 19:00

Practical workshops (P9-P12)  – see outline above

19.00 - 20.00

Bus transfer via ISG to downtown Heidelberg

20:00

Dinner downtown Heidelberg (Restaurant Zum Nepomuk)

 

Individual transport to ISG Hotel

Day 6 - Saturday, 20 October 2012

09:00 - 10:00

Lecture by Rainer Pepperkok “High throughput microscopy approaches to disease mechanism”

10:00 - 13:00

Practical workshops (P9-P12)  – see outline above

13:00 - 14:00

Lunch (EMBL Canteen)

14:00 - 17:00

Practical workshops (P9-P12)  – see outline above

17:00 -18:00

Summary of the course and discussion

18:10

Bus transfer to ISG Hotel