Application for this event is no longer possible.
Parts of the course will be videotaped, and the material will be used to document the course intrinsic knowledge. This gives each participant the unique opportunity to become co-author of a new way of knowledge transfer.
Why You Should Apply
With the help of advanced microscopy techniques this six days practical course is about to learn how to derive qualitative and quantitative insights on molecular mechanisms in cells and developing organisms. Invited guest researchers together with experts from the EMBL and microscopy professionals will allow an intense information flow and a balance of lectures and intense practical workshops . The focus of the course will be on the use of fluorescence microscopy to obtain quantitative information about protein dynamics in living samples.
The topics covered will include all aspects of probe design and fluorescence microscopy imaging and analysis such as: time-lapse microscopy, confocal microscopy, super resolution microscopy and total internal reflection fluorescence microscopy (TIRF).
Special emphasis will be however on quantitative methods such as fluorescence resonance energy transfer (FRET), fluorescence correlation microscopy (FCS) and fluorescence recovery after photobleaching (FRAP). The course will also include state-of-the-art data analysis and image processing techniques.
Who Should Apply
This course is directed towards researchers in the life sciences, with access to light microscopy equipment, and an immediate need to apply the techniques they will learn to solve biological problems at the cellular and molecular level in vivo.