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EMBL Advanced Course

Advanced Imaging Techniques

EMBL Heidelberg, Germany Monday 1 October - Saturday 6 October 2012 Application closed
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Programme

 

Day 1 - Monday, 1 October 2012
9:00 - 9:15 Welcome address
9:15 - 10:00 Overview microscopy techniques
Kai Kuetemeyer
10:00 - 10:15 Coffee break
10:15 - 11:00 Capturing dynamics: Imaging living samples
Stefan Terjung
11:00 - 13:00 Practical session I - Time lapse imaging
13:00 - 14:00 Lunch (Canteen)
14:00 - 15:00 Practical session I (continued - Lunch flexible)
15:00 - 15:30 Coffee break
15:30 - 18:30 Practical session II - Time lapse imaging
18:30 - 19:30 Dinner (Cafeteria)
19:30 - 22:00 Beer & Poster session (Operon Foyer)
 

 

Day 2 - Tuesday, 2 October 2012
09:00 - 09:50 Quantitative FRET recording from sensitized emission

Kees Jalink

09:50 - 10:00 Coffee break
10:00 - 10:50 FRAP to reveal reaction kinetics in living cells
Adriaan Houtsmuller
11:00 - 13:00 Practical session III - FRAP, photoactivation, uncaging
13:00 - 14:00 Lunch (Canteen)
14:00 - 15:00 Practical session III (continued - Lunch flexible)
15:00 - 15:30 Coffee break
15:30 - 18:30 Practical session IV - FRAP, photoactivation, uncaging
18:30 - 19:30 Dinner (Cafeteria)
19:30 - 21:30 FRAP Data analysis session
Adriaan Houtsmuller

 

 

Day 3 - Wednesday, 3 October 2012
09:00 - 09:50 Basics and practical aspects of fluorescence correlation spectroscopy
Malte Wachsmuth
09:50 - 10:00 Coffee break
10:00 - 10:50 Quantitatve microscopy of cell signalling using fluorescence fluctuation microscopy
Mark Hink
11:00 - 13:00 Practical session V - FRET sensitized emission, FCS
13:00 - 14:00 Lunch
14:00 - 15:00 Practical session V (continued - Lunch flexible)
15:00 - 15:30 Coffee break
15:30 - 18:30 Practical session VI - FRET sensitized emission, FCS
18:30 - 19:30 Dinner (Canteen)
19:30 - 21:30 FRET Data analysis session

Kees Jalink

 

 

Day 4 - Thursday, 4 October 2012
09:00 - 09:50 Biological applications of Super-resolution Microscopy

Melike Lakadamyali

09:50 - 10:40 Single-molecule super-resolution, a do-it-yourself tutorial

Ricardo Henriques

10:40 - 11:00

Coffee break

11:00 - 11:45 Fluorescent probe development
Carsten Schultz
11:45 - 12:45 Lunch
12:45 - 15:45 Practical session VII - FRET acceptor photobleaching, Superresolution
15:45 - 16:00 Coffee break
16:00 - 19:00 Practical session VIII - FRET acceptor photobleaching, Superresolution
19:00 - 20:00 Dinner (Cafeteria)
20:00 - 21:30 FCS Data analysis session
Malte Wachsmuth

 

Day 5 - Friday, 5 October 2012
09:00 - 09:50 High throughput microscopy for systems biology
Jan Ellenberg
09:50 - 10:00
Coffee break
10:00 - 10:50 Tracking principles

Kota Miura

11:00 - 13:00 Data analysis
13:00 - 14:00 Lunch
14:00 - 15:30 Data analysis
15:30 - 16:00 Coffee break
16:00 - 18:30 Preparation of data presentations
19:00 - open Course Dinner (in town)

 

 
Day 6 - Saturday, 6 October 2012
09:00 - 10:00 Preparation of data presentations
10:00 - 10:30 Presentations Group 1+2
10:30 - 11:00 Coffee break
11:00 - 11:30 Presentations Group 1+2
11:30 - 12:00 End of course remarks
12:00 - 13:00 Lunch (optional)