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EMBL Introductory Course

Basic Light Microscopy

EMBL Heidelberg, Germany Tuesday 10 July - Friday 13 July 2012 Application closed
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Programme

Tuesday, 10 July 2012

9.00 - 13.00  

Basics of optics and microscopy

   Theoretical basics of light and optics

   Types of microscopes frames  – and types of optics

   Set up bright field microscopes and the appropriate optics for different needs. 

   Cleaning, centering adjusting and operating a microscope

   Basic set up and use of imaging devices 

Hands on session one (step 1-5)

13.00 - 14.00 Lunch
14.00 - 15.00

Contrast methods

   Introduction into contrast methods (phase contrast and differential interference contrast/DIC)      

15.00 - 17.30

Contrast methods - set up and alignment

   Set up and use of contrast methods: phase contrast and differential interference contrast/DIC

   Oblique Illumination or Hoffmann Modulation Contrast - an alternative to DIC?

Hands on session two (step 6-7)

    Discussing open questions

 

Wednesday, 11 July 2012

9.00 - 12.30  

  Basics of digital imaging

   Adaptation of digital cameras and their alignment

   Interpretation of image content, histogram and digital filter functions

   Improving digital image contrast

12.30 - 13.30 Lunch at EMBL canteen
13.30 – 16.00

Fluorescence microscopy I 

   Introduction to fluorescence microscopy

   Setting up of microscopes and cameras for fluorescence observation

   The different types of illumination sources (e.g. mercury, xenon, metal halide or LED)

   Cubes, filters and objectives

   Find the appropriate filter combination for a given fluorochrome and application

Hands on session three (step 8 and groupwork 1-6)

16.00 - 17.00 Discussing open questions

Thursday, 12 July 2012

9.00 - 12.30  

Fluorescence microscopy II 

   Auto-fluorescence – a source for noise

   Digital imaging for fluorescence microscopy

   Specific needs of digital cameras

   Digital multicolor handling

   Acquisition of multidimensional images (3D, time)

Continue of hands on session three (step 8 and groupwork 1-6)

12.30 - 13.30 Lunch at EMBL canteen
13.30 - 14.30 Guided tour at the Advanced light microscopy facility EMBL
14.30 – 16.00 Continue of hands on session three (step 8 and groupwork 1-6)
16.00 - 17.00 Discussing open questions

Friday, 13 July 2012

9.00 - 13.00  

Tutorial on basics in data processing

   3D-data: visualization, projection and rendering

   Deconvolution algorithms and filters

   Time-series: visualization, manual particle tracking and kymographs

13.00 - 14.00    Lunch at EMBL canteen
14.00 - 15.30 Discussing open questions

 

Topics to be covered:

Basics of optics and microscopy
     Theoretical basics of light and optics
     Types of microscopes frames  – and types of optics
     Set up bright field microscopes and the appropriate optics for different needs. 
     Cleaning, centering adjusting and operating a microscope
     Basic set up and use of imaging devices 

Contrast methods
     Introduction into contrast methods (phase contrast and differential interference contrast/DIC)

Contrast methods - set up and alignment
     Set up and use of contrast methods: phase contrast and differential interference contrast/DIC
•     Oblique Illumination or Hoffmann Modulation Contrast - an alternative to DIC?

Basics of digital imaging
     Adaptation of digital cameras and their alignment
     Interpretation of image content, histogram and digital filter functions
     Improving digital image contrast
     Discussing open questions

Fluorescence microscopy I 
     Introduction to fluorescence microscopy
     Setting up of microscopes and cameras for fluorescence observation
     The different types of illumination sources (e.g. mercury, xenon, metal halide or LED)
     Cubes, filters and objectives
     Find the appropriate filter combination for a given fluorochrome and application

Fluorescence microscopy II 
     Auto-fluorescence from the specimen and from the hardware
     Digital imaging for fluorescence microscopy
     Specific needs of digital cameras
     Digital multicolor handling
     Acquisition of multidimensional images (3D, time)

Tutorial on basics in data processing
     3D-data: visualization, projection and rendering
     Deconvolution algorithms and filters
     Time-series: visualization, manual particle tracking and kymographs