EMBL Introductory Course
Basic Light Microscopy
EMBL Heidelberg, Germany Tuesday 10 July - Friday 13 July 2012 Application closed
Programme
Tuesday, 10 July 2012
| 9.00 - 13.00 |
Basics of optics and microscopy • Theoretical basics of light and optics • Types of microscopes frames – and types of optics • Set up bright field microscopes and the appropriate optics for different needs. • Cleaning, centering adjusting and operating a microscope • Basic set up and use of imaging devices Hands on session one (step 1-5) |
|---|---|
| 13.00 - 14.00 | Lunch |
| 14.00 - 15.00 |
Contrast methods • Introduction into contrast methods (phase contrast and differential interference contrast/DIC) |
| 15.00 - 17.30 |
Contrast methods - set up and alignment • Set up and use of contrast methods: phase contrast and differential interference contrast/DIC • Oblique Illumination or Hoffmann Modulation Contrast - an alternative to DIC? Hands on session two (step 6-7) • Discussing open questions |
Wednesday, 11 July 2012
| 9.00 - 12.30 |
Basics of digital imaging • Adaptation of digital cameras and their alignment • Interpretation of image content, histogram and digital filter functions • Improving digital image contrast |
|---|---|
| 12.30 - 13.30 | Lunch at EMBL canteen |
| 13.30 – 16.00 |
Fluorescence microscopy I • Introduction to fluorescence microscopy • Setting up of microscopes and cameras for fluorescence observation • The different types of illumination sources (e.g. mercury, xenon, metal halide or LED) • Cubes, filters and objectives • Find the appropriate filter combination for a given fluorochrome and application Hands on session three (step 8 and groupwork 1-6) |
| 16.00 - 17.00 | Discussing open questions |
Thursday, 12 July 2012
| 9.00 - 12.30 |
Fluorescence microscopy II • Auto-fluorescence – a source for noise • Digital imaging for fluorescence microscopy • Specific needs of digital cameras • Digital multicolor handling • Acquisition of multidimensional images (3D, time) Continue of hands on session three (step 8 and groupwork 1-6) |
|---|---|
| 12.30 - 13.30 | Lunch at EMBL canteen |
| 13.30 - 14.30 | Guided tour at the Advanced light microscopy facility EMBL |
| 14.30 – 16.00 | Continue of hands on session three (step 8 and groupwork 1-6) |
| 16.00 - 17.00 | Discussing open questions |
Friday, 13 July 2012
| 9.00 - 13.00 |
Tutorial on basics in data processing • 3D-data: visualization, projection and rendering • Deconvolution algorithms and filters • Time-series: visualization, manual particle tracking and kymographs |
|---|---|
| 13.00 - 14.00 | Lunch at EMBL canteen |
| 14.00 - 15.30 | Discussing open questions |
Topics to be covered:
Basics of optics and microscopy
• Theoretical basics of light and optics
• Types of microscopes frames – and types of optics
• Set up bright field microscopes and the appropriate optics for different needs.
• Cleaning, centering adjusting and operating a microscope
• Basic set up and use of imaging devices
Contrast methods
• Introduction into contrast methods (phase contrast and differential interference contrast/DIC)
Contrast methods - set up and alignment
• Set up and use of contrast methods: phase contrast and differential interference contrast/DIC
• Oblique Illumination or Hoffmann Modulation Contrast - an alternative to DIC?
Basics of digital imaging
• Adaptation of digital cameras and their alignment
• Interpretation of image content, histogram and digital filter functions
• Improving digital image contrast
• Discussing open questions
Fluorescence microscopy I
• Introduction to fluorescence microscopy
• Setting up of microscopes and cameras for fluorescence observation
• The different types of illumination sources (e.g. mercury, xenon, metal halide or LED)
• Cubes, filters and objectives
• Find the appropriate filter combination for a given fluorochrome and application
Fluorescence microscopy II
• Auto-fluorescence from the specimen and from the hardware
• Digital imaging for fluorescence microscopy
• Specific needs of digital cameras
• Digital multicolor handling
• Acquisition of multidimensional images (3D, time)
Tutorial on basics in data processing
• 3D-data: visualization, projection and rendering
• Deconvolution algorithms and filters
• Time-series: visualization, manual particle tracking and kymographs
