Single-Cell Gene Expression Analysis

Latest News

• Registration is now closed.

• Preliminary Programme is now available.

Why You Should Apply

• Summary
  The molecular analysis of limited biomass from a single cell type can broadly be defined as Microgenomics. This technique is often used to increase the knowledge in areas such as cancer development, plant physiology, neurodegenerative disorders or developmental biology, where the analysis of transcripts in minute samples are necessary. For the success of the technique, homogenous samples of the cell types to be analysed are of primary importance, making a very precise sampling crucial. Depending on the nature of the sample and the goal of the experiment, there are different ways of sampling the probes. This course will present flow cytometry and laser capture microdissection as methods to obtain single cells for the subsequent gene expression analysis. The need for quantitative single-cell mRNA analysis is given by the inability of conventional RNA methods, like northern blotting, RNAse protection assay or classical block RT-PCR, to differentiate mRNA abundance of such limited samples. Another crucial step is the data analysis, which also constitutes a strong component of the course.

• Topics
  • Laser Capture Microdissection
  • Flow Cytometry
  • Single Cell qRTPCR
  • Data Analysis
    

Who Should Apply

This course is designed for scientists who are entering the field of single cell analysis and want to become familiar with the different preparation methods as well as the following gene expression profiling. The course will cover lectures and hands-on sessions by experts in the fields and is aimed for PhD students, postdoctoral fellows or other scientists with a solid qPCR and molecular biology background.