EMBL Advanced Course
Targeted Genome Editing Using
Zinc Finger Nucleases
EMBL Heidelberg, Germany
Wednesday 14 November - Friday 16 November 2012
Application closed
Final Programme
| 09:00 | Welcome |
|---|---|
| Introduction to ZFN Technology | |
| ZFN Design | |
| Genome Editing and Delivery | |
| Nucleofection Overview | |
| Lab Practical: Nucleofection | |
| Double-Strand Break (DSB) Repair | |
| Gene Knockout and Validation | |
| Dinner at EMBL | |
| 20:00 | Bus to Hotel |
| 09:00 | Gene Insertion and Tagging |
|---|---|
| Donor Plasmid Design | |
| Lab Practical: Cel-1 Assay | |
| Integration Detection | |
| Dilution Cloning and Genotyping | |
| Quantitation of ZFN Cel-1 Clevage Activity | |
| Lab Practical: Junction PCR | |
| Dilution Cloning and Genotyping | |
| Lab Practical: RFLP Assay | |
| 17:00 | Bus to hotels |
| 09:00 | Lab Practical: Junction PCR |
|---|---|
| Lab Practical: RFLP Assay | |
| Guest Lecture | |
| Troubleshooting Exercise | |
| Lab Practical: Results | |
| Transgenics | |
| Therapeutics | |
| 17:00 | End of course and bus to train station |
