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This course is aimed at Technicians and Scientists interested in the generation of transgenic animals. Participants should have basic experience with eukaryotic cells and microscopy.
30% of the course program covers lectures and tutorials on generating transgenic mice and corresponding microscopy and laser techniques by experts in the field. 70% focuses on hands on practical work organised in small groups. These practical will address the following topics: injection of DNA into the pronucleus of fertilized mouse oocytes, embryonic stem cell transfer into blastocysts, laser-assisted stem cell transfer into 8-cell stage embryos, and microscopy training.
This course is co-organised with Eppendorf.