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EMBL Advanced Course

Development of Transgenic Animal Models Using Zinc Finger Nucleases

EMBL Heidelberg, Germany Tuesday 26 February - Thursday 28 February 2013 Application closed
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(Preliminary) Programme

Day 1 – Tuesday, 26 February 2013
09:00 Welcome
  Intro to Transgenics
  Overview of ZFNs and Gene Modification
  BREAK
  Pronuclear Injection
  Lab Practical: Microinjection
  LUNCH
  Creating Constitutive Knockout Models
  Lab Practical: Lab Overview, Deletion and Mismatch PCR Setup
17:00 Gene Insertion and Conditional Knockout
17:30 Dinner at EMBL

 

 

Day 2 - Wednesday, 27 February 2013
09:00 Lab Practical: Load deletion PCR gels; Set up Mismatch Formation
  Considerations for Cell-based Transgenics
  BREAK
  Donor Design
  ZFN Design
  LUNCH
  Lab Practical: Mismatch Detection Assay & PAGE & Troubleshooting
  Talen Technology: What's the difference?
  BREAK
17:00 Lab Practical: Set up Junction & RFLP PCR
19:00 Dinner downtown

 

 

Day 3 - Thursday, 28 February 2013
09:00 Lab Practical: Load jPCR gel; Cleanup RFLP & Digest
  In vivo Therapeutic Applications of ZFN
  SAGE Labs Transgenic Model Creation Service
  Group Photo
  Lab Practical: Run RFLP gel
  Guest Lecture
  LUNCH
  Exercises for ZFN Application
  Experimental Results and Analysis
16:00 Closeout