EMBL Advanced Course
Development of Transgenic Animal Models Using Zinc Finger Nucleases
EMBL Heidelberg, Germany Tuesday 26 February - Thursday 28 February 2013 Application closed
(Preliminary) Programme
| 09:00 | Welcome |
|---|---|
| Intro to Transgenics | |
| Overview of ZFNs and Gene Modification | |
| BREAK | |
| Pronuclear Injection | |
| Lab Practical: Microinjection | |
| LUNCH | |
| Creating Constitutive Knockout Models | |
| Lab Practical: Lab Overview, Deletion and Mismatch PCR Setup | |
| 17:00 | Gene Insertion and Conditional Knockout |
| 17:30 | Dinner at EMBL |
| 09:00 | Lab Practical: Load deletion PCR gels; Set up Mismatch Formation |
|---|---|
| Considerations for Cell-based Transgenics | |
| BREAK | |
| Donor Design | |
| ZFN Design | |
| LUNCH | |
| Lab Practical: Mismatch Detection Assay & PAGE & Troubleshooting | |
| Talen Technology: What's the difference? | |
| BREAK | |
| 17:00 | Lab Practical: Set up Junction & RFLP PCR |
| 19:00 | Dinner downtown |
| 09:00 | Lab Practical: Load jPCR gel; Cleanup RFLP & Digest |
|---|---|
| In vivo Therapeutic Applications of ZFN | |
| SAGE Labs Transgenic Model Creation Service | |
| Group Photo | |
| Lab Practical: Run RFLP gel | |
| Guest Lecture | |
| LUNCH | |
| Exercises for ZFN Application | |
| Experimental Results and Analysis | |
| 16:00 | Closeout |
