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EMBO Practical Course

High Throughput Microscopy for Systems Biology

EMBL Heidelberg, Germany Monday 20 October - Sunday 26 October 2014 Application deadline: Friday 8 August 2014
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  • Registration has been extended until 8 August 2014, please register here. Please don't forget to submit your motivation letter. Applications without motivation letter will not be considered.

Why you should apply

High-throughput techniques such as protein analysis by mass spectrometry, expression and transcription profiling by protein or DNA microarrays or high throughput DNA, RNA sequencing are successfully applied to diverse biological questions with the goal of reaching a comprehensive description of their molecular regulation. However, they cannot provide temporal or spatial resolution nor directly show that the identified molecules have a functional role in the cellular process investigated.

Quantitative fluorescence-microscopy in living cells overcomes these limitations because it can probe the function of macromolecules in living cells with ever increasing spatial and temporal resolution. Simple imaging assays have recently been demonstrated to enable genome-wide functional analyses by high-throughput-microscopy. The availability of standardized reagents that interfere with cellular functions (e.g. siRNAs) together with new GFP-tagging and transgene approaches that allow direct characterization of the abundance, localization, dynamics and interaction of the proteins in intact cells, promise now to giving rise to imaging-based proteomics.

High-throughput microscopy that can provide data for such systems-biology questions requires a high level of automation, quantification and precise coordination of various steps in an integrated workflow.

In this course, we will teach most recent developments in high-throughput fluorescence microscopy, which is becoming a key technology for systems biology.

 

 

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