- Registration is closed. If you are interested to join this course, please contact Christina Dollt.
- All fellowships have been allocated. Successful participants have been notified.
- A shuttle bus will be running from the ISG Hotel to EMBL and back. Its schedule can be viewed here.
The field of DNA sequencing has evolved rapidly over the last years and the rise of next generation sequencing (NGS) has caused a dramatic decrease in sequencing costs. Nowadays NGS is not only applied in basic research studies but also in translational and clinical settings. Targeted resequencing panel approaches usually use either PCR amplicon or enrichment based technologies to generate sequence ready libraries which are then analyzed using a benchtop sequencer.
The goal of this course is to teach the fundamentals of NGS library preparation and validation using an Amplicon PCR based test like the TruSeq Amplicon assay. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and normalize the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of TruSeq Amplicon sequencing results using standard Illumina tools like MiSeq Reporter and VariantStudio.
During this course we will focus on the following aspects:
- Wet lab training and best practices for TruSeq Amplicon library preparation
- Best practices of sequencing library validation and bead-based normalization
- Custom assay design using DesignStudio
- Introduction to Amplicon data analysis and interpretation using Illumina MiSeq Reporter and VariantStudio software
Who should apply?
This course is directed towards molecular biologists, scientists and technicians who want to apply Amplicon based targeted sequencing in their lab. Knowledge of next generation sequencing technology desirable is but not essential.
This course is co-organised with Illumina.