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Non-coding RNAs have shot to prominence as key regulatory molecules involved in development, differentiation, and metabolism of most eukaryotic organisms. Investigating non-coding RNA function is important for our understanding of these biological processes and also for a range of disease states. In addition, it is now obvious that changes in expression profiles of non-coding RNAs can serve as clinically relevant biomarkers and aid in the early detection of disease. The course, Non-coding RNAs: From discovery to function, is designed to introduce researchers to the theoretical and practical aspects of the methods used for discovery and profiling of non-coding RNA expression such as in-situ hybridization, microarray and qPCR analyses, fluorescent sorting and massively parallel sequencing.
Aims and Topics
The course will be focused on the theoretical and practical aspects of methods used for discovery and profiling of non-coding RNAs including in-situ hybridization, qPCR analyses, fluorescent sorting and massively parallel sequencing. This will be accomplished through practical exercises using real experimental samples from ongoing research projects. This will be followed by training in approaches to assess microRNA function. Training will also include data analysis, miRNA target prediction, correlation of mRNA and miRNA expression profiles as well as correlation of data obtained by various methods.
During the course we will pay particular attention to following aspects:
- Good scientific and laboratory practice in experimental design, preparation of samples and their quality assessment as well as critical evaluation of data;
- Introduction and explanation of different approaches and instrumentation systems used for studying noncoding RNAs;
- Detailed troubleshooting;
- Comparison of analysis strategies and evaluation of obtained data;
- Importance of standardized approach to miRNA expression profiling and suitable normalization strategies.
Who should apply?
The course is open to students at an advanced stage of their post-graduate work (Ph.D training) and post-doctoral fellows.