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In the past few years extracellular vesicles (EV) have increasingly attracted the attention of scientists worldwide. Recent research has shown that these small vesicles act as messengers conveying information not only to neighboring cells, but also to distant tissues in eukaryotes, and between cells in prokaryotes. Containing cell-specific payloads of proteins, lipids and genetic material, they are able to alter function and physiology of target cells. Their importance in pathophysiological processes is just beginning to unravel and thus, also their biomedical relevance.
A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles (with practical sessions included).
Extracellular vesicles purification (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques)
Extracellular vesicles characterisation (Immunoblot and electron microscopy, Quantitative and qualitative analysis by flow cytometry, Structural and mechanical characterisation by atomic force microscopy, Nanoparticle Tracking Analysis)
Extracellular vesicles composition (Lipidomics, Proteomics, Transcriptomics)
Extracellular vesicles biogenesis and function (Molecular players for extracellular vesicles biogenesis, Extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)
Who should apply?
This course is intended for PhD and post-doctoral researchers entering the field.