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EMBO Practical Course

Single Molecule and Single Cell Fluorescence Å/nm/μm/mm-scopy

EMBL Heidelberg, Germany Sunday 15 March - Monday 23 March 2015 Application deadline: Sunday 21 December 2014
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  • Registration is live!
  • Preliminary Programme available online

Why apply?

  • Topics
    • Structural Biology & Biophysics
    • Cell & Tissue Architecture

  • Summary
    Fluorescence is a light-based molecular phenomenon that is inherently non-invasive; it thus finds broad application in the studies of living matter - from the observation of isolated proteins in buffer to complex machinery in living animals. Detection of fluorescence offers highest signal to noise, such that even single molecules can be investigated. This coined the development of myriads of fluorescence-based experimental strategies in biology and biophysics. Progress in biological research is directly linked to the development and subsequent application of new spectroscopic/microscopic techniques. Researchers in biology are mostly question driven – their aiming at using novel light-microscopy often reflects the hope to gain new insights, based on the promises of publication on the methods. This typically causes a misperception, e.g. that certain experiments performed under controlled biochemical conditions might not be easily adaptable to studies in cells, or that high-resolution methods do frequently not match the demands of in cell studies. Vice versa, in cell studies frequently lack the insights into the molecular mechanism. To achieve the necessary understanding to integrate seamlessly fluorescence tools across different resolution levels and laboratory scales (biochemical-cell-organism) in their research projects, the participants will receive hands on training and specialized training across a large range of high-resolution fluorescence studies. In particular, the course aims to teach the common concepts but also what requirements distinguish a large set of advanced fluorescent spectroscopic and microscopic techniques, from biochemical conditions to complex cellular systems. Experts in different fields will introduce principles, challenges and guidance of their fields. In this surrounding the participants will be able to directly perform small research projects in teams of two people on state of the art equipment and discuss their results with invited investigators, instructors, local teachers and editor.

Who should apply?

Participants will be advanced PhD students or postdocs. They will be selected based on scientific excellence, judged from their application, CV and the thereof derived estimation of the immediate impact this training will have on their current projects and future career perspective.