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In Life Sciences, imaging in three dimensions is a prerequisite for the understanding of the fine organisation of cells and tissues. Whilst key biological functions are addressed by a large choice of fluorescence microscopy techniques, volume imaging by scanning electron microscopy offers now an unprecedented understanding of the ultrastructure of large biological objects. Correlating both imaging modalities, in 3D, is therefore a powerful way to link function to structure, even in complex biological systems.
This workshop will gather leading experts in the field of 3D correlative light and electron microscopy, with a particular focus on automated serial imaging by scanning electron microscopy. Two keynotes, from W. Denk and F. Hamprecht will cover the latest breakthroughs in automated serial imaging and 3D image analysis. The symposium will then alternate lectures and practical workshops to address a large spectrum of topics related to 3D CLEM, from the state of the art to recent biological applications.
Volume imaging and 3D correlative approaches are developing fast with increasing number of users and applications. This symposium aims not only at providing an update on the latest development in the field but also at connecting people. Experts and new-to-the-field will have the opportunity to share their experience and their views on the current methods and tools, but also exchange about the future challenges of these emerging techniques.
The session topics are as follows:
- 3D Correlative Light and Electron Microscopy
- The challenges of sample diversity
- From large volume imaging to data analysis and processing
- How 3D CLEM connects to other 3D Imaging Techniques
- Diversity of applications in biology
The workshops will include:
- Sample Preparation
- Array Tomography
- Image Processing
In addition there will be three panel discussions on broad topics within the scope of 3D Correlative Light and Electron Microscopy. This will be an opportunity to raise questions to experts in the field. We are encouraging participants to post questions on each of these topics before the event so that the session can cover the most pressing issues. The topics will be:
- Large Data Handling
- Shared experiences in CLEM
- Facility Implementation – tips and tricks for implementing these techniques in a facility environment, marketing facility services, charging for services, equipment maintenance and consumables.
Participants will have the choice to attend all 6 workshops, or to substitute one or more workshops with panel discussions.
This workshop will be followed by a Gatan 3view Users Forum at EMBL.
This workshop is jointly organised by ZEISS and EMBL