These options control formatting of alignments in results pages. The default is HTML, but other formats (including plain text) are available. PSSM and PssmWithParameters are representations of Position Specific Scoring Matrices and are only available for PSI-BLAST. The Advanced view option allows the database descriptions to be sorted by various indices in a table. more...
Choose how to view alignments. The default "pairwise" view shows how each subject sequence aligns individually to the query sequence. The "query-anchored" view shows how all subject sequences align to the query sequence. For each view type, you can choose to show "identities" (matching residues) as letters or dots. more...
Show only sequences from the given organism.
Show only those sequences that match the given Entrez query. more...
Show only sequences with expect values in the given range. more...
Your BLAST job specified more than one input sequence. This box lets you choose which input sequence to show BLAST results for.
An overview of the database sequences aligned to the query sequence is shown. The score of each alignment is indicated by one of five different colors, which divides the range of scores into five groups. Multiple alignments on the same database sequence are connected by a striped line. Mousing over a hit sequence causes the definition and score to be shown in the window at the top, clicking on a hit sequence takes the user to the associated alignments. New: This graphic is an overview of database sequences aligned to the query sequence. Alignments are color-coded by score, within one of five score ranges. Multiple alignments on the same database sequence are connected by a dashed line. Mousing over an alignment shows the alignment definition and score in the box at the top. Clicking an alignment displays the alignment detail.
This dot matrix view shows regions of similarity based upon the BLAST results. The query sequence is represented on the X-axis and the numbers represent the bases/residues of the query. The subject is represented on the Y-axis and again the numbers represent the bases/residues of the subject. Alignments are shown in the plot as lines. Plus strand and protein matches are slanted from the bottom left to the upper right corner, minus strand matches are slanted from the upper left to the lower right. The number of lines shown in the plot is the same as the number of alignments found by BLAST.
Score E Sequences producing significant alignments: (Bits) Value lcl|50681 1ton Rat Tonin KLK2_RAT 180 2e-50
>lcl|50681 1ton Rat Tonin KLK2_RAT Length=235 Score = 180 bits (457), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 95/235 (40%), Positives = 130/235 (55%), Gaps = 13/235 (5%) Query 1 IVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEG 60 IVGGY C N+ P+QV++ + Y CGG LI+ WV++AAHCY + QV LG +N+ E Sbjct 1 IVGGYKCEKNSQPWQVAVINEY-LCGGVLIDPSWVITAAHCYSNNYQVLLGRNNLFKDEP 59 Query 61 NEQFISASKSIVHPSY-----------NSNTLNNDIMLIKLKSAASLNSRVASISLPTSC 109 Q +S HP Y + +ND+ML+ L A + V I LPT Sbjct 60 FAQRRLVRQSFRHPDYIPLIVTNDTEQPVHDHSNDLMLLHLSEPADITGGVKVIDLPTKE 119 Query 110 ASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGG 169 G+ CL SGWG+T S L+C+ +LS+ C Y +T M CAG +EGG Sbjct 120 PKVGSTCLASGWGSTNPSEMVVSHDLQCVNIHLLSNEKCIETYKDNVTDVMLCAGEMEGG 179 Query 170 KDSCQGDSGGPVVCSGKLQGIVSWG-SGCAQKNKPGVYTKVCNYVSWIKQTIASN 223 KD+C GDSGGP++C G LQGI S G + CA+ P +Y K+ + SWIK+ + N Sbjct 180 KDTCAGDSGGPLICDGVLQGITSGGATPCAKPKTPAIYAKLIKFTSWIKKVMKEN 234
BLAST Basic Local Alignment Search Tool
BLAST Basic Local Alignment Search Tool
