Biocomputing Unit
Biocomputing
Sequence Analysis Service
Gibson Group
EMBL
EMBL

Predocs05 Course

Basic Tools in Sequence Analysis Practical

by Toby Gibson and the Sequence Analysis Team, October 7th-10th, 2005

Answers to Questions


Part 1. WWW sequence similarity searching

Step 2 BLAST2 searching with human SM-B protein

Questions and Answers

Step 3 BLAST 2 search with SM-B and a filter

Questions and Answers


Part 2. Exploring protein architecture and function with SMART, GlobPlot and ELM

Step 1. Comparing a sequence to a database of protein domains

Questions and Answers


Step 2. Exploring order/disorder with Globplot

Questions and Answers


Step 3. Searching for short functional sites with the ELM server

Questions and Answers


Step 4. Exploring the architecture of the protein Epsin1.

Questions and answers


Part 3. Making multiple sequence alignments and calculating a tree.

Step 2. Aligning the elongation factor sequences with Clustal X

Questions and answers


Step 3. Calculating a tree with Clustal X and displaying it with NJplot

Questions and answers