http://genome.cbs.dtu.dk/services/NetGene2/
NetGene2 World Wide Web Server
Center for Biological Sequence Analysis
The Technical University of Denmark
DK-2800 Lyngby, Denmark
Performing prediction for HSAK1 (size: 12229) Species: H. sapiens
Starting prediction in + strand
- Get coding activities of networks ......
- Get donor activities of networks ............
- Get acceptor activities of networks ............
- Get branchpoint activities of networks ..
- Get derivatives of coding prediction ..........
- Find splice site reading frame ....................
- Get confidence values ....................
- Get new confidence values ....................
Starting prediction in - strand
- Get coding activities of networks ......
- Get donor activities of networks ............
- Get acceptor activities of networks ............
- Get branchpoint activities of networks ..
- Get derivatives of coding prediction ..........
- Find splice site reading frame ....................
- Get confidence values ....................
- Get new confidence values ....................
Preparing output
- Dump all prediction data ..........
- Dump all prediction data ..........
- Make inputfile for geneplot ..........
- Plotting + strand ...............................
- Make inputfile for geneplot ..........
- Plotting - strand ...............................
Prediction done
********************** NetGene2 v. 2.4 ************************
The sequence: HSAK1 has the following composition:
Length: 12229 nucleotides.
19.8% A, 28.3% C, 31.7% G, 20.3% T, 0.0% X, 60.0% G+C
Donor splice sites, direct strand
---------------------------------
pos 5'->3' phase strand confidence 5' exon intron 3'
158 0 + 0.65 CTCCCGCAAG^GTATTTGACA
236 1 + 0.63 GCTTAGCACA^GTGAGTCGTC
447 2 + 0.67 GGAGTCCCAG^GTGGGTCCTG
984 2 + 0.51 CCCTCCCCAG^GTCAGTGCGT
2466 1 + 0.32 CAATAGCAAG^GTGGGGAAAG
2627 0 + 0.32 TTGTAAGCGG^GTGTGTGATG
2755 0 + 0.47 GGACATAGTG^GTGAGGAGAC
3723 1 + 0.76 TCTGTGACAG^GTGAGGCCAT
3751 2 + 0.34 GAGAGGAAAG^GTGACTTTTC
3896 1 + 0.83 TTAGTGGAAG^GTGAGTCAGG
3906 2 + 0.63 GTGAGTCAGG^GTGAGGAGGA
3989 0 + 0.47 AGGATGGAAG^GTATGAGCCT
4669 0 + 0.46 CCAGCTTATG^GTGAGGGCCC
5202 1 + 0.31 AGAGCCAGAG^GTGACCGGCC
5566 0 + 0.70 CATCTTTGTG^GTGGGTGAGT
5570 1 + 0.99 TTTGTGGTGG^GTGAGTTGCG H
5906 0 + 1.00 GGTTCCACTG^GTGAGTGGGC H
5925 1 + 0.71 CCCTGGTGGG^GTGAGAGGCA
6766 2 + 0.00 AAGAGTTTGA^GCGACGGGTA
6773 0 + 1.00 TGAGCGACGG^GTAAGGCACT H
6864 0 + 0.34 TGTGATGCAG^GTCAGAGTTC
8772 0 + 0.71 ACCAGAGACT^GTGAGTCTGT
8822 2 + 0.55 ATGATGGGGG^GTGGGTGACC
8914 2 + 0.32 CCGCTGGGTG^GTACGGAGTC
9057 2 + 0.34 AGAGGCAGGG^GTGAGCCTGG
9073 2 + 0.41 CTGGGTCCAG^GTCAGTCTGA
10267 0 + 0.89 TGTGCGCAAG^GTGGGCCCCG H
10452 0 + 0.34 GCCTGCCATG^GTGAGGCCCA
11603 1 + 0.41 ATGGTCAGGG^GTGAGACCTG
Donor splice sites, complement strand
-------------------------------------
pos 3'->5' pos 5'->3' phase strand confidence 5' exon intron 3'
11911 319 1 - 0.47 TTAAGGACAC^GTGAGCACCA
11704 526 2 - 0.54 TGACCTTGAG^GTGGGGGTGA
10805 1425 0 - 0.45 TTGCGGCCAG^GTAGGCACAA
10490 1740 1 - 0.55 GAGATGGGCC^GTGAGGCTGA
9503 2727 1 - 0.34 GAGGGAAGGG^GTGAGGGGCT
8703 3527 0 - 0.32 AGCAGAGGTG^GTAAATCTGG
8029 4201 0 - 0.63 CACACAGCTG^GTATGTGTGA
7920 4310 1 - 0.47 GATAGGGAAG^GTGAGGGGCA
7623 4607 0 - 0.45 TGACACCGAG^GTAAAAGTGT
7532 4698 2 - 0.63 ATGTGCAGGA^GTGAGTATCC
5806 6424 2 - 0.92 CGGTGGAGAG^GTGGGTGTAG H
5370 6860 0 - 0.63 CAGCAGGGAT^GTGAGTGAGC
4967 7263 1 - 0.39 AGAGAGGGCG^GTGAGCTTGG
4324 7906 0 - 0.58 TCCCGGAGAG^GTACGGGGAA
4151 8079 1 - 0.37 GGCACATCCC^GTGAGAGGCC
3866 8364 2 - 0.31 AAGGCAACTG^GTGTGTGCCA
3516 8714 1 - 0.45 CTGCCAGGAG^GTGGGAGGTG
2549 9681 2 - 0.59 ATAGGTCCCG^GTGAGCTGAC
1250 10980 1 - 0.31 GAACAAAGGG^GTGACTATCT
653 11577 1 - 0.47 TGTGCTCAAG^GTACAAGGCC
366 11864 0 - 0.71 AGACCACCCA^GTAAGGTAGA
361 11869 2 - 0.71 ACCCAGTAAG^GTAGAAGATT
27 12203 - - 0.00 CCCAGATGAG^GTGGGTTGGA
Acceptor splice sites, direct strand
------------------------------------
pos 5'->3' phase strand confidence 5' intron exon 3'
302 2 + 0.27 CTATCCACAG^CCTTCTGAAT
634 2 + 0.15 GCTATCATAG^GGAAAGCCTG
1224 0 + 0.25 TGTGTTGCAG^CCCCTTCCAG
1353 2 + 0.25 CCTGCCCCAG^AGGACCTGGA
1388 1 + 0.27 TGCTCCGTAG^GCTATCGTGT
1734 1 + 0.33 GGCCCCACAG^GCCCCTTAGC
2238 0 + 0.33 TCCTCCCAAG^ATGGCAGTGA
2374 0 + 0.17 AGGTTTCCAG^GAGGCACCTG
2377 0 + 0.17 TTTCCAGGAG^GCACCTGCCA
2388 2 + 0.07 CACCTGCCAG^TGTGGGAGTG
2867 0 + 0.27 CTTGCCATAG^GGGCTGGAGC
2989 2 + 0.28 GCCTGCACAG^GGATGGGGGT
3446 1 + 0.16 TTCACCCCAG^GCCTCTGTCC
3526 2 + 0.33 CTCCTGGCAG^CTTCCCTCTG
3547 2 + 0.07 CCCTGGGGAG^CGTTGTCAAG
3841 1 + 0.57 CTGCCCTCAG^ACTGCATGTC
3949 2 + 0.65 TGTCTTACAG^AGCACTGACA
4469 1 + 0.07 CTGCTGGCAG^CACCATCCGT
4881 0 + 0.07 CTCTCTCGAG^CTCAGTTTCC
4886 2 + 0.29 TCGAGCTCAG^TTTCCCTCTG
4901 2 + 0.55 CTCTGCCCAG^TGGGGCTGTG
4931 2 + 0.07 GATCTAACAG^CAGGCAGGAC
4934 2 + 0.14 CTAACAGCAG^GCAGGACGAG
5137 0 + 0.17 GACACGGCAG^GACGGGACCA
5178 1 + 0.25 ACCCCCGCAG^AGAAGACGAT
5353 0 + 0.49 CCTCTCCCAG^GCTCCGAGCT
5414 0 + 0.56 TCCCTGACAG^GAGCGCCAGC
5533 1 + 1.00 TCTCCCGCAG^AGAAGCTGAA H
5535 0 + 0.93 TCCCGCAGAG^AAGCTGAAGA
5538 0 + 0.25 CGCAGAGAAG^CTGAAGAAAA
5544 0 + 0.18 GAAGCTGAAG^AAAACCAAGA
5741 1 + 0.43 GCGTTTGCAG^GTGGGCCTGG
5756 1 + 0.34 CCTGGCTCAG^GGAAGGGCAC
5761 0 + 0.31 CTCAGGGAAG^GGCACCCAGT
5770 0 + 0.19 GGGCACCCAG^TGTGAGAAGA
5776 0 + 0.19 CCAGTGTGAG^AAGATCGTGC
5779 0 + 0.18 GTGTGAGAAG^ATCGTGCAGA
5788 0 + 0.07 GATCGTGCAG^AAGTATGGCT
5970 2 + 0.17 GTCCCACCAG^CAGCAAAGCC
5973 2 + 0.17 CCACCAGCAG^CAAAGCCCAC
5978 2 + 0.07 AGCAGCAAAG^CCCACGGCAC
6159 0 + 0.15 GTCCCCTCAG^ACTAGCCATA
6267 0 + 0.33 TCTACTCCAG^AAGCCCCACT
6351 1 + 0.16 CCCTCACCAG^CCCCTGAGCT
6367 2 + 0.33 AGCTCCCCAG^GGCAGGGAAG
6494 0 + 0.25 CTCAGTCCAG^GTCTCCACAA
6655 0 + 0.97 TGTGCCCCAG^GAGACAGTGT H
6658 0 + 0.44 GCCCCAGGAG^ACAGTGTTGG
6662 1 + 0.42 CAGGAGACAG^TGTTGGACAT
7314 2 + 0.16 CTCCTTCCAG^GGGTCATGAG
7795 2 + 0.33 CGTCTCGTAG^GGCCTTCTGG
7986 1 + 0.23 CCTGTGTTAG^GTGCCACAGT
8407 2 + 0.31 ACCCTTCCAG^GGCTGTTGTA
8472 0 + 0.82 TGGCCTCCAG^GTGTCCGGGC
8484 0 + 0.07 GTCCGGGCAG^CTGGTCTTCT
8639 2 + 0.16 GTCCTGACAG^GGAGACTGAG
8676 1 + 0.07 TCAAGCCCAG^AGGCTGAGCT
8678 0 + 0.07 AAGCCCAGAG^GCTGAGCTGG
8684 0 + 0.18 AGAGGCTGAG^CTGGCTCTCC
8697 1 + 0.35 GCTCTCCCAG^ATTTACCACC
8727 1 + 0.19 GTGTGTAAAG^AGCCTACTGT
8729 0 + 0.18 GTGTAAAGAG^CCTACTGTGC
8861 1 + 0.14 CTGCATCCAG^GCCCAGCCTT
8867 1 + 0.18 CCAGGCCCAG^CCTTTGCCCC
9117 2 + 0.26 CTCTGTCCAG^CTGTGTGGCC
9419 2 + 0.43 CCTTTCCAAG^GCGTCCCATG
9535 1 + 0.25 TTTGTCAAAG^GACCCCAGAA
9574 2 + 0.33 CTGCCCTCAG^GGCCCTTGGG
10074 0 + 0.85 CCGCCTGCAG^ATTGGACAGC
10083 0 + 0.55 GATTGGACAG^CCCACACTGC
10111 1 + 0.30 GTGGACGCAG^GCCCTGAGAC
10119 0 + 0.07 AGGCCCTGAG^ACCATGACCC
10484 1 + 0.19 GGTGACTCAG^CCTCACGGCC
10507 0 + 1.00 CTCCCCACAG^GTCAACGCTG H
10519 0 + 0.19 CAACGCTGAG^GGCTCCGTGG
10546 0 + 0.33 CTTCTCCCAG^GTCTGCACCC
10776 1 + 0.56 CTGCTTCCAG^TTGGAGTTGA
10849 2 + 0.15 TCCTCCTCAG^CCTCCCTTTG
10913 0 + 0.53 CCTCGCACAG^GGCATGCCTG
10979 2 + 0.17 TCATGTTCAG^GCAGAACCGG
10983 0 + 0.07 GTTCAGGCAG^AACCGGTCCC
11710 1 + 0.32 CCACCTCAAG^GTCAGGATGC
11715 0 + 0.34 TCAAGGTCAG^GATGCGAACA
11736 0 + 0.19 ACTGAGTGAG^GAGTGGACGC
Acceptor splice sites, complement strand
----------------------------------------
pos 3'->5' pos 5'->3' phase strand confidence 5' intron exon 3'
11771 459 1 - 0.85 TGGTCTCCAG^CCCTCATGGC
11203 1027 0 - 0.17 CCCTTTTCAG^ACCCTCAGAG
11195 1035 2 - 0.56 AGACCCTCAG^AGCCTCTCGC
10670 1560 0 - 0.43 TGCTCAGCAG^GGCAGGGTGG
10582 1648 0 - 0.07 GCGGCTCCAG^CGTTGCTACT
10568 1662 2 - 0.40 GCTACTTTAG^GGCGTCCAGG
10559 1671 2 - 0.18 GGGCGTCCAG^GTGGGTGCAG
10549 1681 0 - 0.18 GTGGGTGCAG^ACCTGGGAGA
9817 2413 2 - 0.25 TCTGCTCTAG^GCTCTCATCA
9790 2440 2 - 0.15 TCCATGCCAG^ACCTGTGCTC
9589 2641 2 - 0.56 CCTTAAGCAG^GTCTCCCAAG
9153 3077 1 - 0.17 CTCTGTCCAG^CTGTGTAACC
9140 3090 2 - 0.19 TGTAACCAAG^AGGCTTGTCC
9138 3092 1 - 0.19 TAACCAAGAG^GCTTGTCCAA
9127 3103 0 - 0.20 CTTGTCCAAG^GCCACACAGC
9118 3112 0 - 0.20 GGCCACACAG^CTGGACAGAG
9110 3120 2 - 0.19 AGCTGGACAG^AGGCGAGAAG
9108 3122 1 - 0.18 CTGGACAGAG^GCGAGAAGCA
8966 3264 0 - 0.31 TCCTCCGTAG^TTAAGGATTC
8940 3290 2 - 0.07 ACAGCGTCAG^CTGCCTTCAG
8930 3300 0 - 0.25 CTGCCTTCAG^GGAGATGACT
8926 3304 1 - 0.19 CTTCAGGGAG^ATGACTCCGT
8907 3323 2 - 0.19 TACCACCCAG^CGGCTGCTTC
8887 3343 1 - 0.85 TACCTTACAG^ATTACCATGG
8633 3597 0 - 0.16 TCCCTGTCAG^GACAATGGAC
8523 3707 0 - 0.19 TTCGGTGAAG^CCCTGGCGCC
8500 3730 0 - 0.14 TGGTGTCCAG^AGCCAAGAAG
8068 4162 1 - 0.43 TATTTTCTAG^ATGTGGCCCT
7930 4300 1 - 0.25 CCCATTTCAG^ATAGGGAAGG
7878 4352 0 - 0.15 GGTCTTGCAG^CCACTCAGCA
7857 4373 0 - 0.07 CCACTCGGAG^GTCAGAAAAT
7852 4378 2 - 0.07 CGGAGGTCAG^AAAATATGGC
7829 4401 0 - 0.28 CTATCCCCAG^ATGAGGCTGG
7672 4558 0 - 0.16 TCCCTCCTAG^GCCCTTGCAG
7639 4591 0 - 0.43 TCCCATACAG^CCTTGTGACA
7600 4630 1 - 0.25 GTGTGCACAG^GGCCATGTTG
7329 4901 0 - 0.17 TTCCAGCCAG^GCTCCTCATG
7161 5069 1 - 0.33 GTGTTTATAG^GGAGCCAAGG
7062 5168 1 - 0.69 CATTTTGCAG^ATGGGAAAAC
6928 5302 1 - 0.33 ACACTTCCAG^AAAGTCTTCC
6799 5431 1 - 0.16 AGCCCACCAG^GATCCCCACT
6781 5449 1 - 0.07 CTTGGGTCAG^TGCCTTACCC
6730 5500 1 - 0.17 CCGCGGGTAG^CCATCAATCA
6597 5633 0 - 0.25 CTTCTCCCAG^CCTCTCGTCA
6520 5710 2 - 0.43 CCCCCTCCAG^GAAGCTTCCT
6422 5808 1 - 0.31 TTCCAGCCAG^GTGCTGTCCT
5917 6313 0 - 0.82 ACCCCACCAG^GGCCCACTCA
5903 6327 2 - 0.34 CACTCACCAG^TGGAACCAGC
5894 6336 2 - 0.34 GTGGAACCAG^CTGCCCCTTC
5867 6363 2 - 0.74 TTTCCGACAG^CTTCTTGCCC
5539 6691 2 - 0.30 TTTTCTTCAG^CTTCTCTGCG
5269 6961 0 - 0.16 CTCCACACAG^CCAGCGGGCT
5189 7041 0 - 0.33 TGGCTCTCAG^ATCGTCTTCT
5099 7131 0 - 0.71 CCCCTCTCAG^GCCCTGGGCC
4925 7305 1 - 0.43 CTGCTGTTAG^ATCCCAGCAA
4808 7422 1 - 0.17 GGGCTTTCAG^GCTCCCCCGC
4791 7439 1 - 0.28 CGCTTCCCAG^GGAAATAGAG
4705 7525 2 - 0.25 CCTTCCCCAG^GGAGGAGGTG
4071 8159 2 - 0.33 CCCCTCCCAG^AATGTCTCCC
3593 8637 0 - 0.27 AAACTCCCAG^AAAACAGGGC
3355 8875 0 - 0.33 ACCTCTACAG^GCCCAACAAC
3224 9006 2 - 0.29 GCTGCCACAG^CCCCCAGGAC
3217 9013 0 - 0.23 CAGCCCCCAG^GACCTCCCCT
3143 9087 0 - 0.27 TCACTCCCAG^CCACCAGCCC
2953 9277 1 - 0.25 CCAGCCCCAG^ACATCCATGC
2814 9416 1 - 0.33 CTCTCCACAG^CCCTGCACAC
2778 9452 0 - 0.43 CCCTCCCCAG^GCTCTCTCCG
2588 9642 0 - 0.33 CGGCTCCCAG^ACAAAGCCTG
2382 9848 1 - 0.25 ACACTGGCAG^GTGCCTCCTG
1309 10921 0 - 0.15 TGGGCCCAAG^CTGGGACATG
1020 11210 2 - 0.43 CTCCCCGCAG^AGGCGCCCCC
735 11495 1 - 0.16 GGACCCCCAG^GTGGCTGTGC
421 11809 0 - 0.22 CCTGTTGGAG^GGACCCTTCG
409 11821 0 - 0.17 ACCCTTCGAG^CTCCTCTGGG
------------------------------------------------------------------------------
CUTOFF values used for confidence:
Highly confident donor sites (H): 95.0 %
Nearly all true donor sites: 50.0 %
Highly confident acceptor sites (H): 95.0 %
Nearly all true acceptor sites: 20.0 %
Graphics showing the prediction output
Direct strand ( + strand)
Complement strand ( - strand)
Graphics in postscript format
Direct Strand 98255 bytes.
Complement Strand 98687 bytes.
Prediction scores files
Direct Strand 131671 bytes.
Complement Strand 130392 bytes.