Here are some protocols commonly used in the Levine lab:

Collecting embryos

Probe making

In situ hybridization (ISH) protocol

Antibody staining protocol – colorimetric (2 days)

Antibody staining protocol – fluorescent (1 day)

Tyramide Signal Amplification (TSA)

Injection plasmid prep and injections

Protein purification

Dialysis protocol

Immunoprecipitation protocol

Plasmid prep (3-solutions method)

Buffers / Solutions / Media

The Levine Lab moved to 585 LSA,

HERE are some pics of the move & new lab.

HERE are some pics of "crazy Mike" and lab members more recently.