Publications of the Pepperkok Lab Home !



Simpson JC, Mateos A, Pepperkok R.

Maturation of the mammalian secretome.

Genome Biol. 2007 Apr 30;8(4):211.


Erfle H, Neumann B, Liebel U, Rogers P, Held M, Walter T, Ellenberg J, Pepperkok R.

Reverse transfection on cell arrays for high content screening microscopy.

Nat Protoc. 2007;2(2):392-9.


Simpson JC, Cetin C, Erfle H, Joggerst B, Liebel U, Ellenberg J, Pepperkok R.

An RNAi screening platform to identify secretion machinery in mammalian cells.

J Biotechnol. 2007 Apr 30;129(2):352-65.


Erfle H, Pepperkok R.

Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells.

Methods Mol Biol. 2007;360:155-61.


Laketa V, Simpson JC, Bechtel S, Wiemann S, Pepperkok R.

High-content microscopy identifies new neurite outgrowth regulators.

Mol Biol Cell. 2007 Jan;18(1):242-52.


Grabher C, Cliffe A, Miura K, Hayflick J, Pepperkok R, Rorth P, Wittbrodt J.

Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka.

J Leukoc Biol. 2007 Jan;81(1):263-71.




Stauber T, Simpson JC, Pepperkok R, Vernos I.

A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex.

Curr Biol. 2006 Nov 21;16(22):2245-51.


Brunet S, Zimmermann T, Reynaud EG, Vernos I, Karsenti E, Pepperkok R.

Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion.

Traffic. 2006 Sep;7(9):1283-9.


Simpson JC, Pepperkok R.

The subcellular localization of the mammalian proteome comes a fraction closer.

Genome Biol. 2006;7(6):222.


Pepperkok R, Ellenberg J.

High-throughput fluorescence microscopy for systems biology.

Nat Rev Mol Cell Biol. 2006 Sep;7(9):690-6.


Colombelli J, Pepperkok R, Stelzer EH, Reynaud EG.

Laser nanosurgery in cell biology.

Med Sci (Paris). 2006 Jun-Jul;22(6-7):651-8.


Runz H, Miura K, Weiss M, Pepperkok R.

Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G.

EMBO J. 2006 Jul 12;25(13):2953-65.


Neumann B, Held M, Liebel U, Erfle H, Rogers P, Pepperkok R, Ellenberg J.

High-throughput RNAi screening by time-lapse imaging of live human cells.

Nat Methods. 2006 May;3(5):385-90.


Verissimo F, Silva E, Morris JD, Pepperkok R, Jordan P.

Protein kinase WNK3 increases cell survival in a caspase-3-dependent pathway.

Oncogene. 2006 Jul 13;25(30):4172-82.


Forster R, Weiss M, Zimmermann T, Reynaud EG, Verissimo F, Stephens DJ, Pepperkok R.

Secretory cargo regulates the turnover of COPII subunits at single ER exit sites.

Curr Biol. 2006 Jan 24;16(2):173-9.


Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S.

The LIFEdb database in 2006.

Nucleic Acids Res. 2006 Jan 1;34(Database issue):D415-8.


Simpson JC, Nilsson T, Pepperkok R.

Biogenesis of tubular ER-to-Golgi transport intermediates.

Mol Biol Cell. 2006 Feb;17(2):723-37.




Pepperkok R, Simpson JC, Rietdorf J, Cetin C, Liebel U, Terjung S, Zimmermann T.

Imaging platforms for measurement of membrane trafficking.

Methods Enzymol. 2005;404:8-18.


Erfle H, Pepperkok R.

Arrays of transfected mammalian cells for high content screening microscopy.

Methods Enzymol. 2005;404:1-8.


Colombelli J, Reynaud EG, Rietdorf J, Pepperkok R, Stelzer EH.

In vivo Selective Cytoskeleton Dynamics Quantification in Interphase Cells Induced by Pulsed Ultraviolet Laser Nanosurgery.

Traffic. 2005 Dec;6(12):1093-102.


Reynaud EG, Andrade MA, Bonneau F, Ly TB, Knop M, Scheffzek K, Pepperkok R.

Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization.

BMC Biol. 2005 Oct 7;3:21.


Arlt D, Huber W, Liebel U, Schmidt C, Majety M, Sauermann M, Rosenfelder H, Bechtel S, Mehrle A, Bannasch D, Schupp I, Seiler M, Simpson JC, Hahne F, Moosmayer P, Ruschhaupt M, Guilleaume B, Wellenreuther R, Pepperkok R, Sultmann H, Poustka A, Wiemann S.

Functional profiling: from microarrays via cell-based assays to novel tumor relevant modulators of the cell cycle.

Cancer Res. 2005 Sep 1;65(17):7733-42.


Tabary O, Boncoeur E, de Martin R, Pepperkok R, Clement A, Schultz C, Jacquot J.

Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells.

Cell Signal. 2006 May;18(5):652-60.


Knauer SK, Moodt S, Berg T, Liebel U, Pepperkok R, Stauber RH.

Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions.

Traffic. 2005 Jul;6(7):594-606.


Keese M, Magdeburg RJ, Herzog T, Hasenberg T, Offterdinger M, Pepperkok R, Sturm JW, Bastiaens PI.

Imaging epidermal growth factor receptor phosphorylation in human colorectal cancer cells and human tissues.

J Biol Chem. 2005 Jul 29;280(30):27826-31.


Yang YD, El Amawi R, Bubeck J, Pepperkok R, Ritzenthaler C, Robinson DG.

Visualization of COPII and Golgi Dynamics in Nicotiana tabacum BY-2 Cells Provides Evidence for Transient Association of Golgi Stacks with ER Exit Sites.

Plant Cell. 2005 May;17(5):1513-31.


Neubrand VE, Will RD, Mobius W, Poustka A, Wiemann S, Schu P, Dotti CG, Pepperkok R, Simpson JC.

gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking.

EMBO J. 2005 Mar 23;24(6):1122-33.


Ben-Tekaya H, Miura K, Pepperkok R, Hauri HP.

Live imaging of bidirectional traffic from the ERGIC.

J Cell Sci. 2005 Jan 15;118(Pt 2):357-67.


Watson P, Forster R, Palmer KJ, Pepperkok R, Stephens DJ.

Coupling of ER exit to microtubules through direct interaction of COPII with dynactin.

Nat Cell Biol. 2005 Jan;7(1):48-55.


Young J, Stauber T, del Nery E, Vernos I, Pepperkok R, Nilsson T.

Regulation of microtubule-dependent recycling at the trans-golgi network by Rab6A and Rab6A'.

Mol Biol Cell. 2005 Jan;16(1):162-77.




Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sultmann H, Poustka A.

From ORFeome to biology: a functional genomics pipeline.

Genome Res. 2004 Oct;14(10B):2136-44.


Erfle H, Simpson JC, Bastiaens PI, Pepperkok R.

siRNA cell arrays for high-content screening microscopy.

Biotechniques. 2004 Sep;37(3):454-8, 460, 462.


Starkuviene V, Liebel U, Simpson JC, Erfle H, Poustka A, Wiemann S, Pepperkok R.

High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic.

Genome Res. 2004 Oct;14(10A):1948-56.


Brunet S, Sardon T, Zimmerman T, Wittmann T, Pepperkok R, Karsenti E, Vernos I.

Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts.

Mol Biol Cell. 2004 Dec;15(12):5318-28.


Stephens DJ, Pepperkok R.

Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum.

J Cell Sci. 2004 Jul 15;117(Pt 16):3635-44.


Becker S, Simpson JC, Pepperkok R, Heinz S, Herder C, Grez M, Seifried E, Tonn T.

Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells.

Thromb Haemost. 2004 Jul;92(1):23-35.


Conrad C, Erfle H, Warnat P, Daigle N, Lorch T, Ellenberg J, Pepperkok R, Eils R.

Automatic identification of subcellular phenotypes on human cell arrays.

Genome Res. 2004 Jun;14(6):1130-6.


Liebel U, Kindler B, Pepperkok R.

'Harvester': a fast meta search engine of human protein resources.

Bioinformatics. 2004 Aug 12;20(12):1962-3.


Bannasch D, Mehrle A, Glatting KH, Pepperkok R, Poustka A, Wiemann S.

LIFEdb: a database for functional genomics experiments integrating information from external sources, and serving as a sample tracking system.

Nucleic Acids Res. 2004 Jan 1;32(Database issue):D505-8.




Simpson JC, Pepperkok R.

Localizing the proteome.

Genome Biol. 2003;4(12):240.


Wiemann S, Bechtel S, Bannasch D, Pepperkok R, Poustka A; German cDNA Network.

The German cDNA network: cDNAs, functional genomics and proteomics.

J Struct Funct Genomics. 2003;4(2-3):87-96.


Liebel U, Starkuviene V, Erfle H, Simpson JC, Poustka A, Wiemann S, Pepperkok R.

A microscope-based screening platform for large-scale functional protein analysis in intact cells.

FEBS Lett. 2003 Nov 20;554(3):394-8.


Zimmermann T, Rietdorf J, Pepperkok R.

Spectral imaging and its applications in live cell microscopy.

FEBS Lett. 2003 Jul 3;546(1):87-92.




Zimmermann T, Rietdorf J, Girod A, Georget V, Pepperkok R.

Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair.

FEBS Lett. 2002 Nov 6;531(2):245-9.


Gruss OJ, Wittmann M, Yokoyama H, Pepperkok R, Kufer T, Sillje H, Karsenti E, Mattaj IW, Vernos I.

Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells.

Nat Cell Biol. 2002 Nov;4(11):871-9.


Richards AA, Stang E, Pepperkok R, Parton RG.

Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection.

Mol Biol Cell. 2002 May; 13(5):1750-64.


Stephens DJ, Pepperkok R.

Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells.

J Cell Sci. 2002 Mar 15;115(Pt6):1149-60.


Runz H, Rietdorf J, Tomic I, de Bernard M, Beyreuther K, Pepperkok R, Hartmann T.

Inhibition of intracellular cholesterol transport alters presenilin localization and amyloid precursor protein processing in neuronal cells.

J Neurosci. 2002 Mar 1;22(5):1679-89.




Kerkhoff E, Simpson JC, Leberfinger CB, Otto IM, Doerks T, Bork P, Rapp UR, Raabe T, Pepperkok R.

The Spir actin organizers are involved in vesicle transport processes.

Curr Biol. 2001 Dec 11;11(24):1963-8.


Stephens DJ, Pepperkok R.

The many ways to cross the plasma membrane.

Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4295-8.


Stephens DJ, Pepperkok R.

Illuminating the secretory pathway: when do we need vesicles?

J Cell Sci. 2001 Mar;114(Pt 6):1053-9.


Pepperkok R, Simpson JC, Wiemann S.

Being in the right location at the right time.

Genome Biol. 2001;2(9):REVIEWS1024.


Simpson JC, Neubrand VE, Wiemann S, Pepperkok R.

Illuminating the human genome.

Histochem Cell Biol. 2001 Jan;115(1):23-9.




Bastiaens PI, Pepperkok R.

Observing proteins in their natural habitat: the living cell.

Trends Biochem Sci. 2000 Dec;25(12):631-7.


Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S.

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.

EMBO Rep. 2000 Sep;1(3):287-92.


Storrie B, Pepperkok R, Nilsson T.

Breaking the COPI monopoly on Golgi recycling.

Trends Cell Biol. 2000 Sep;10(9):385-91.


Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP.

COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites.

J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85.


Pepperkok R, Hotz-Wagenblatt A, Konig N, Girod A, Bossemeyer D, Kinzel V.

Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidation.

J Cell Biol. 2000 Feb 21;148(4):715-26.


Denzel A, Otto F, Girod A, Pepperkok R, Watson R, Rosewell I, Bergeron JJ, Solari RC, Owen MJ.

The p24 family member p23 is required for early embryonic development.

Curr Biol. 2000 Jan 13;10(1):55-8.


Pepperkok R, Whitney JA, Gomez M, Kreis TE.

COPI vesicles accumulating in the presence of a GTP restricted arf1 mutant are depleted of anterograde and retrograde cargo.

J Cell Sci. 2000 Jan;113 ( Pt 1):135-44.




Heiss NS, Girod A, Salowsky R, Wiemann S, Pepperkok R, Poustka A.

Dyskerin localizes to the nucleolus and its mislocalization is unlikely to play a role in the pathogenesis of dyskeratosis congenita.

Hum Mol Genet. 1999 Dec;8(13):2515-24.


Girod A, Storrie B, Simpson JC, Johannes L, Goud B, Roberts LM, Lord JM, Nilsson T, Pepperkok R.

Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum.

Nat Cell Biol. 1999 Nov;1(7):423-30.


Gillham H, Golding MC, Pepperkok R, Gullick WJ.

Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling.

J Cell Biol. 1999 Aug 23;146(4):869-80.


Shima DT, Scales SJ, Kreis TE, Pepperkok R.

Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes.

Curr Biol. 1999 Jul 29-Aug 12;9(15):821-4.


Pepperkok R, Squire A, Geley S, Bastiaens PI.

Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy.

Curr Biol. 1999 Mar 11;9(5):269-72.


Jackson ME, Simpson JC, Girod A, Pepperkok R, Roberts LM, Lord JM.

The KDEL retrieval system is exploited by Pseudomonas exotoxin A, but not by Shiga-like toxin-1, during retrograde transport from the Golgi complex to the endoplasmic reticulum.

J Cell Sci. 1999 Feb;112 ( Pt 4):467-75.