An evolutionary surprise
The marine worm Platynereis has a muscle (red) which develops in the same place and has the same genetic signature as the notochord (blue) that develops into our spinal discs. Credit: Kalliopi Monoyios
- Notochord (1st vertebrate skeleton) probably evolved from muscle
- Evolutionary origin of notochord likely older than assumed
- Marine worm has muscle with same genetic signature, in same place
Thoughts of the family tree may not be uppermost in the mind of a person suffering from a slipped disc, but those spinal discs provide a window into our evolutionary past. They are remnants of the first vertebrate skeleton, whose origins now appear to be older than had been assumed. Scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, have found that, unexpectedly, this skeleton most likely evolved from a muscle. The study, carried out in collaboration with researchers at the Howard Hughes Medical Institute in Janelia Farm, USA, is published today in Science.
Humans are part of a group of animals called chordates, whose defining feature is a rod of cartilage that runs lengthwise along the middle of their body, under their spinal chord. This structure, called the notochord, was the first vertebrate skeleton. It is present in human embryos, and is replaced with the backbone as we develop, with the cartilage reduced to those tell-tale discs. Since starfish, sea urchins and related animals have no such structure, scientists assumed the notochord had emerged in a relatively recent ancestor, after our branch of the evolutionary tree split away from the ‘starfish branch’.
“People simply haven’t been looking beyond our direct relatives, but that means you could be fooled, if the structure appeared earlier and that single group lost it,” says Detlev Arendt from EMBL, who led the study. “And in fact, when we looked at a broader range of animals, this is what we found.”
Antonella Lauri and Thibaut Brunet, both in Arendt’s lab, identified the genetic signature of the notochord – the combination of genes that have to be turned on for a healthy notochord to form. When they found that the larva of the marine worm Platynereis has a group of cells with that same genetic signature, the scientists teamed up with Philipp Keller’s group at Janelia Farm to use state-of-the-art microscopy to follow those cells as the larva developed. They found that the cells form a muscle that runs along the animal’s midline, precisely where the notochord would be if the worm were a chordate. The researchers named this muscle the axochord, as it runs along the animal’s axis. A combination of experimental work and combing through the scientific literature revealed that most of the animal groups that sit between Platynereis and chordates on the evolutionary tree also have a similar, muscle-based structure in the same position.
The scientists reason that such a structure probably first emerged in an ancient ancestor, before all these different animal groups branched out on their separate evolutionary paths. Such a scenario would also explain why the lancelet amphioxus, a ‘primitive’ chordate, has a notochord with both cartilage and muscle. Rather than having acquired the muscle independently, amphioxus could be a living record of the transition from muscle-based midline to cartilaginous notochord.
The shift from muscle to cartilage could have come about because a stiffened central rod would make swimming more efficient, the scientists postulate.
Lauri, A., Brunet, T., Handberg-Thorsager, M., Fischer, A.H.L., Simakov, O., Steinmetz, P.R.H., Tomer, R., Keller, P.J. & Arendt, D. Development of the annelid axochord: Insights into notochord evolution. Science, 12 September 2014. DOI: 10.1126/science.1253396.
The origin of chordates has been debated for more than a century, with one key issue being the emergence of the notochord. In vertebrates, the notochord develops by convergence and extension of the chordamesoderm, a population of midline cells of unique molecular identity. We identify a population of mesodermal cells in a developing invertebrate, the marine annelid Platynereis dumerilii, that converges and extends toward the midline and expresses a notochord-specific combination of genes. These cells differentiate into a longitudinal muscle, the axochord, that is positioned between central nervous system and axial blood vessel and secretes a strong collagenous extracellular matrix. Ancestral state reconstruction suggests that contractile mesodermal midline cells existed in bilaterian ancestors. We propose that these cells, via vacuolization and stiffening, gave rise to the chordate notochord.
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