Fluorescent Dyes:
Introduction - Quantifying calcium - Definitions&References - Protocols&Examples

Protocols & Examples

Material and solutions used in this tutorial are detailed here. Some tips to adapt them to each experiment have been included.

The following protocols are routinely used in our lab for dyes loading and were also used for the examples shown.

- Loading Fluo-4 AM in HeLa cells

- Loading Indo-1 AM in HeLa cells

Due to the high variability in calcium quantification experiments, these protocols can be used as a starting point, but they should be optimized for each particular experiment.

Some tips to chose and setup the microscope can be found here.



Fluorescent Proteins


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