Fluorescent Proteins:
Introduction - Quantifying calcium - Definitions - Protocols & Examples
 

Protocols & Examples

Material and solutions used for cell transfection and Fluorescent Protein experiments are detailed here.

The following protocols are routinely used in our lab for YCs transfection and were also used for the examples shown.

- YCs expressed in cytosol

- YCs expressed in endoplasmic reticulum

Due to the high variability in calcium quantification experiments, these protocols can be used as a starting point, but they should be optimized for each particular experiment.

Some tips to chose and setup the microscope can be found here.

 

 

Fluorescent Dyes

 

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