Postdoctoral Fellow
Location: Rome, Italy
Staff Category: Postdoctoral Fellow
Contract Duration: 3 years
Grading: Postdoctoral fellow
Closing Date: 30 June 2021
Reference Number: RM00096
The Genetic & Viral Engineering Facility (GAVEF) at the Neurobiology and Epigenetics Unit of EMBL Rome, Italy, is looking for a Postdoctoral Fellow who will participate in the development of genome engineering technologies. This individual will be embedded in an interdisciplinary team of young scientists working within a highly collaborative international environment. GAVEF specialises in the design, engineering, and production of viral vectors including rAAV and lentivirus. In addition to these custom-made viral vectors and stocks, we produce a wide range of DNA targeting constructs for genome engineering including reagents for CRISPR/Cas9. Your role will not be service provision but will instead center on developing technologies that would be useful to both our facility and the accompanying Genetic Embryology and Engineering Facility (GEEF). This postdoc will bridge the expertise of these two facilities and potentially create a template for future facilities. Additionally, you will benefit from being part of a collaboration with Vilnius University (Lithuania) to analyze multiple novel CRISPR systems as part of the technology development.

Your role

To meet the research goals of the facility, the person appointed will directly take part in projects to produce novel rAAV vectors and optimize their use to produce both transgenic somatic cells and mice either genetically or epigenetically. Cutting edge molecular biology techniques including recombineering will be used to engineer the vectors. A pipeline for production of vectors, testing and optimization of the viral constructs will be developed in both cells and mice. You will work closely with the head of both GAVEF and GEEF on these projects but will report to the head of GAVEF. You are expected to take a strong experimental lead and will be expected to develop independent concepts as the project proceeds.

You have

• PhD in Molecular Biology, virology or related subjects. • Broad knowledge in genetic engineering and strong interest in technology development and optimization of protocols. • Full training and/or extensive working experience in molecular biology including experience in DNA manipulation, plasmid preps, PCR, qPCR, cloning, and restriction mapping. • Hands-on experience in manipulating bacteria and cell culture with aseptic technique. • Experience in mammalian cell tissue culture, ideally mES cells. • Excellent organizational skills, ability to run multiple projects in parallel. • Outstanding project management skills and ability to work independently. • Expertise maintaining a clear, organised, up to date notebook and needed electronic databases. • Good working knowledge of common desktop software applications. • Excellent communication, teamwork, and interpersonal skills with highest ethical standards. • High motivation and enthusiasm to work within an international team. • Fluency in written and spoken English.

You might also have

• Experience in viral cultivation, purification and quantitation as well as work under BSL2 conditions. • Experience of IVF and/or manipulating one cell mouse embryos. • Imaging live cells/embryos via fluorescence microscopy. • Experience with DNA analysis/documentation software such as SnapGene or Gene Construction Kit. • Experience with designing CRISPR reagents. • Skills in using, maintaining and improving databases including FileMaker and Excel. • A good command of Italian is beneficial but not required; the working language is English.

Why join us

Why not? The European Molecular Biology Laboratory (EMBL) is an inclusive, equal opportunity employer offering attractive conditions and benefits appropriate to an international research organization with a very collegial and family friendly working environment. The remuneration package comprises a competitive salary, a comprehensive pension scheme, medical, and other social benefits, as well as financial support for relocation and installation, including your family.

What else you need to know

EMBL is one of the highest ranked scientific research organizations in the world. The Headquarters Laboratory is located in Heidelberg (Germany), with additional sites in Grenoble (France), Hamburg (Germany), Hinxton (UK), Barcelona (Spain), and Rome (Italy). The Neurobiology and Epigenetics Unit at EMBL Rome studies various mammalian physiological phenomena from molecular to system levels using the laboratory mouse as a model system. We take advantage of a full range of core facilities as well as close institutional links with other EMBL sites.