mcf_s

The Metabolomics Core Facility provides services for the analysis of metabolites and lipids.

The facility is equipped with a high-resolution mass spectrometry system (HRMS)triple quadrupole with an ion trap (QTRAP) mass spectrometer coupled with ultra-high performance liquid chromatography (UHPLC),  and direct infusion system (NanoMate) for separation and detection of various classes of small molecules, metabolites, lipids, and drugs.

Major projects and accomplishments

  • Identification and detection of various classes of small molecules, metabolites, and lipids in mammalian and microbial cells

  • EMBL-MCF mass spectral library from over 700 metabolites

  • Lipidomics profiling platform for comprehensive analysis of lipid species from multiple lipid classes (e.g. glycerols (TGs, DGs), phospholipids, free fatty acids, etc)

  • Developed protocols to analyze over 100 metabolites in a targeted manner

  • Relative quantitation of metabolites and lipids using internal standards

  • Analysis of drugs and related metabolites

Services provided

We provide services for mass spectrometry-based analysis of small molecules, metabolites and lipids. We perform targeted and untargeted measurements of various classes of molecules ranging from small metabolites to lipids in a variety of biological matrices.

Further services

  • Global untargeted metabolomics and lipidomics profiling
  • Targeted metabolomics profiling
  • Free fatty acid analysis
  • Metabolite and lipid identification using MS and MS/MS analysis

  • Drug and drug metabolite analysis
  • Low-volume high-throughput direct infusion analysis (with Advion Nanomate)

Acknowledgment and Authorship:

As per EMBL Internal Publication Policy (No. 63); if the facility services were used in  work described in a publication, the facility must be acknowledged. For acknowledgment, the following statement must be added:

“We acknowledge the support of the EMBL Metabolomics Core Facility  (MCF)  in the acquisition and analysis of mass spectrometry data”

In addition, If a staff member of the Metabolomics Core Facility contributes substantially to published work, the staff member should be included as an author in the published work.

Services

Contact us for more information

This website offers guidelines for sample submission to the MCF and provides practical information on sample preparation and submission. Should this not give you the information you are looking for, MCF staff are always available to answer your questions and advise on sample preparation.