Q-Exactive Plus (ThermoFisher) high-resolution mass spectrometer (HRMS)
Advanced hybrid quadrupole-Orbitrap mass spectrometer with high resolution up to 280,000, fast scan speed, and HCD fragmentation. This HRMS system can be coupled either to UHPLC or to direct infusion for metabolomic applications such as detection, identification, and quantification of small molecules, metabolites, and lipids (Services).
This system is especially good for untargeted analysis of metabolites and lipids in the discovery phase.
Advanced high pressure and performance liquid chromatography system for separation of small molecules, metabolites, and lipids, used in combination with HRMS.
NanoChip-based electrospray ionization technology which combines LC, chip-based infusion, fraction collection, and direct surface analysis with the mass spec (Q-exactive plus) into one integrated system. It improves the high throughput and sensitivity of the analysis.
Triple Quadrupole with and Ion Trap (QTRAP 6500+ from Sciex)
(Advanced mass-spectrometer with built-in triple quadrupole and q-trap capabilities)
This instrument has fast scan speeds for high-throughput analysis with millisecond polarity switching. This mass spectrometer has several targeted capabilities including multiple reaction monitoring (MRM) for quantitation of metabolites and lipids), constant neutral loss (CNL), and a precursor and product ion scans. This mass spectrometer also has MS3 capabilities for the qualitative analysis of metabolites and lipids.
The QTRAP system in the facility instrument can be coupled to either an LC (Exion) and to a differential ion mobility spectrometry (DMS) device.
This instrument is particularly good for hypothesis-driven studies, for analysis of low concentration metabolites, and, for metabolite quantitation.