Vector Promoter Selection; Tags and Fusion partners Protease cleavage site Origin Supplier
pALTER-Ex1 T7 Tet none none
Promega
pALTER-Ex2 T7 Tet none none
Promega
pBAD/His araBAD Amp N-His EK pUC Invitrogen
pBAD/Myc-His araBAD Amp C-His none pUC Invitrogen
pBAD/gIII araBAD Amp leader sequence
C-His
none ColE1 Invitrogen
pCal-n T7-lac Amp N-CBP Thr ColE1 Stratagene
pCal-n-EK T7-lac Amp N-CBP Thr
EK  
ColE1 Stratagene
pCal-c T7-lac Amp C-CBP Thr ColE1 Stratagene
pCal-Kc T7-lac Amp C-CBP Thr ColE1 Stratagene
pcDNA 2.1 T7 Amp none none pUC Invitrogen
pDUAL T7-lac Kan C-CBP Thr ColE1 Stratagene
pET-3a-c T7 Amp none none pBR322 Novagen
pET-9a-d T7 Kan none none pBR322 Novagen
pET-11a-d T7-lac Amp none none pBR322 Novagen
pET-12a-c T7 Amp none none pBR322 Novagen
pET-14b T7 Amp N-His Thr pBR322 Novagen
pET-15b T7-lac Amp N-His Thr pBR322 Novagen
pET-16b T7-lac Amp N-His Xa pBR322 Novagen
pET-17b T7 Amp none none pBR322 Novagen
pET-19b T7-lac Amp N-His EK pBR322 Novagen
pET-20b(+) T7 Amp signal sequence
C-His
none pBR322 Novagen
pET-21a-d(+) T7-lac Amp C-His none pBR322 Novagen
pET-22b(+) T7-lac Amp signal sequence
C-His
none pBR322 Novagen
pET-23a-d(+) T7 Amp C-His none pBR322 Novagen
pET-24a-d(+) T7-lac Kan C-His none pBR322 Novagen
pET-25b(+) T7-lac Amp signal sequence
C-His
none pBR322 Novagen
pET-26b(+) T7-lac Kan Signal sequence
C-His
none pBR322 Novagen
pET-27b(+) T7-lac Kan signal sequence
C-His
none pBR322 Novagen
pET-28a-c(+) T7-lac Kan N-His
C-His
Thr pBR322 Novagen
pET-29a-c(+) T7-lac Kan C-His Thr pBR322 Novagen
pET-30a-c(+) T7-lac Kan N-His
C-His
Thr
EK  
pBR322 Novagen
pET-31b(+) T7-lac Amp C-His none pBR322 Novagen
pET-32a-c(+) T7-lac Amp internal His
C-His
Thr
EK  
pBR322 Novagen
pET-33b(+) T7-lac Kan internal His
C-His
Thr
EK  
pBR322 Novagen
pET-34b(+) T7-lac Kan N-CBDclos
C-His
Thr
EK  
pBR322 Novagen
pET-35b(+) T7-lac Kan N-CBDclos
C-His
Thr
Xa  
pBR322 Novagen
pET-36b(+) T7-lac Kan signal sequence
N-CBDcenA
C-His
Thr
EK  
pBR322 Novagen
pET-37b(+) T7-lac Kan signal sequence
N-CBDcenA
C-His
Thr
Xa  
pBR322 Novagen
pET-38b(+) T7-lac Kan signal sequence
C-CBDcex
C-His
Thr pBR322 Novagen
pET-39b(+) T7-lac Kan (signal sequence)
DsbA
N-His
C-His
Thr
EK  
pBR322 Novagen
pET-40b(+) T7-lac Kan (signal sequence)
DsbC
N-His
C-His
Thr
EK  
pBR322 Novagen
pET-41a-c(+) T7-lac Kan GST
N-His
C-His
Thr
EK  
pBR322 Novagen
pET-42a-c(+) T7-lac Kan GST
N-His
C-His
Thr
Xa  
pBR322 Novagen
pET-43a-c(+) T7-lac Kan NusA
N-His
C-His
Thr
EK  
pBR322 Novagen
pETBlue-1 T7-lac Amp C-His none pUC Novagen
pETBlue-2 T7-lac Amp C-His none pUC Novagen
pETBlue-3 T7-lac Amp N-His
C-His
Thr
EK  
pUC Novagen
pGEMEX-1 T7 Amp T7 gene 10 none
Promega
pGEMEX-2 T7 Amp T7 gene 10 none
Promega
pGEX-1lT tac Amp GST Thr pBR322 Pharmacia
pGEX-2T tac Amp GST Thr pBR322 Pharmacia
pGEX-2TK tac Amp GST Thr
EK  
pBR322 Pharmacia
pGEX-3X tac Amp GST Xa pBR322 Pharmacia
pGEX-4T tac Amp GST Thr pBR322 Pharmacia
pGEX-5X tac Amp GST Xa pBR322 Pharmacia
pGEX-6P tac Amp GST Pre pBR322 Pharmacia
pHAT10/11/12 lac Amp N-HAT EK pUC Clontech
pHAT20 lac Amp N-HAT EK pUC Clontech
pHAT-GFPuv lac Amp N-HAT
N-GFPuv
EK pUC Clontech
pKK223-3 tac Amp none none pBR322 Pharmacia
pLEX PL  Amp none none pUC Invitrogen
pMAL-c2X tac Amp N-MBP Xa ColE1 NEB
pMAL-c2E tac Amp N-MBP EK ColE1 NEB
pMAL-c2G tac Amp N-MBP Gen ColE1 NEB
pMAL-p2X tac Amp N-MBP Xa ColE1 NEB
pMAL-p2E tac Amp N-MBP EK ColE1 NEB
pMAL-p2G tac Amp N-MBP Gen ColE1 NEB
pProEX HT trc Amp N-His TEV
Life Technologies
pPROLar.A lac-ara1 Kan N-c-Myc EK p15A Clontech
pPROTet.E LtetO-1 Cam N-c-Myc EK ColE1 Clontech
pQE-9 T5-lac Amp N-His none ColE1 Qiagen
pQE-16 T5-lac Amp C-His none ColE1 Qiagen
pQE-30/31/32 T5-lac Amp N-His none ColE1 Qiagen
pQE-40 T5-lac Amp N-His
N-DHFR
none ColE1 Qiagen
pQE-60 T5-lac Amp C-His none ColE1 Qiagen
pQE-70 T5-lac Amp C-His none ColE1 Qiagen
pQE-80/81/82L T5-lac Amp N-His none ColE1 Qiagen
pQE-100 T5-lac Amp N-His none ColE1 Qiagen
pRSET T7 Amp N-His EK ColE1 Invitrogen
pSE280 trc Amp none none pUC Invitrogen
pSE380 trc Amp none none pUC Invitrogen
pSE420 trc Amp none none pUC Invitrogen
pThioHis trc Amp His-Patch Trx EK ColE1 Invitrogen
pTrc99A trc Amp none none pBR322 Pharmacia
pTrcHis trc Amp N-His EK pUC Invitrogen
pTrcHis2 trc Amp C-His none pUC Invitrogen
pTriEx-1 T7 Amp C-His none pUC Novagen
pTriEx-2 T7-lac Amp N-His
C-His
Thr
EK  
pUC Novagen
pTrxFus PL  Amp Trx EK ColE1 Invitrogen