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Tag or fusion partner size location expression secretion purification detection
His-tag 6 or 10 aa N, C, internal

X  X 
Flag-tag 8 aa N, C

X 
T7-tag 11 or 16 aa N, internal X 
X 
S-tag 15 aa N, C, internal

X  X 
HSV-tag 11 aa C

X 
B-tag (VP7 protein region of bluetongue virus)



X 
Strep-tag



X  X 
polyarginine 5-15 aa C (refolding)
X 
polycysteine 4 aa N

X 
polyphenylalanine 11 aa N

X 
polyaspartic acid 5-16 aa C

X 
(Ala-Trp-Trp-Pro)n



X 
Thioredoxin 109 aa
(11.7 kDa)
N, C X 
X 
b-Galactosidase 116 kDa N, C X 
X  X 
Chloramphenicol acetyltransferase 24 kDa N
X  X  X 
Cyclomaltodextrin gluconotransferase



X 
CTP:CMP-3-deoxy-D-manno-octulosonate cytidyltransferase (CKS)

X 


trpE or trpLE 27 kDa N X 
X 
Avidin


X  X  X 
Streptavidin


X  X  X 
T7 gene 10 260 aa N X 
X 
T4 gp55

X 


Staphylococcal protein A 14 (or 31) kDa N X  X  X 
Streptococcal protein G 28 kDa N, C X  X  X 
Glutathione-S-transferase (GST) 26 kDa N X 
X 
Dihydrofolate reductase (DHFR) 25 kDa N

X 
Cellulose-binding domain (CBP) 107, 114 or 156 aa N, C
X  X 
Maltose-binding domain (MBP) 40 kDa N, C X  X  X 
Galactose-binding domain



X 
Calmodulin-binding domain (CBP) 4 kDa N, C

X  X 
Chitin-binding domain
N, C X 


Hemagglutinin influenza virus (HAI epitope)



X  X 
Green fluorescent protein (GFP) 220 aa N, C


X 
KSI 125 aa N X 


c-myc



X  X 
OmpT 22 aa N
X 

OmpA 21 aa N
X 

PelB 20 aa N
X 

Disulfide oxidoreductase (DsbA) 208 aa N X  X 

Disulfide isomerase (DsbC) 236 aa N X  X 

NusA 495 aa N X 


Ubiquitin 76 aa N X 


lac-repressor



X 
Growth hormone N-terminus

X 


Hemolysin A (E. coli)



X 
cII protein

X