Vector
Map

Seq

Promoter
Selection
Tag
Protease cleavage site
Host
Origin
Source
Description
pACYC177 mapseq   kan
amp
none none DH5a p15A New England Biolabs cloning vector with p15A origin
pASK75 map

seq

tet amp C-Strep none BL21 pBR322 Biometra expression vector
pBAD/His A map

seq

araBAD amp N-His EK TOP10
LMG194
pUC Invitrogen expression vector
pBAD/His B map

seq

araBAD amp N-His EK TOP10
LMG194
pUC Invitrogen expression vector
pBAD/His C map

seq

araBAD amp N-His EK TOP10
LMG194
pUC Invitrogen expression vector
pBAD/MCS map

seq

araBAD amp none none TOP10
LMG194
pUC A. Geerlof expression vector
pBADM-11 map

seq

araBAD amp N-His TEV TOP10
LMG194
pUC A. Geerlof expression vector
pBADM-20 map

seq

araBAD amp N-TrxA
N-His
TEV TOP10
LMG194
pUC A. Geerlof expression vector
pBADM-20(+) map

seq

araBAD amp N-TrxA
N-His
TEV TOP10
LMG194
pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-30 map

seq

araBAD amp N-His
N-GST
TEV TOP10
LMG194
pUC A. Geerlof expression vector
pBADM-30(+) map

seq

araBAD amp N-His
N-GST
TEV TOP10
LMG194
pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-41(+) map

seq

araBAD amp N-His
N-MBP
TEV TOP10
LMG194
pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-52 map

seq

araBAD amp N-
LL-
DsbA
N-His
TEV TOP10
LMG194
pUC A. Geerlof expression vector
pBADM-52(+) map

seq

araBAD amp LL-DsbA
N-His
TEV TOP10
LMG194
pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-60 map

seq

araBAD amp N-NusA
N-His
TEV TOP10
LMG194
pUC A. Geerlof expression vector
pBADM-60(+) map

seq

araBAD amp N-NusA
N-His
TEV TOP10
LMG194
pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBAT4 map

seq

T7 amp none none BL21(DE3) pUC M. Hyvönen expression vector
pBAT5 map

seq

T7 amp none none BL21(DE3) pUC M. Hyvönen expression vector
pCal-n map

seq

T7/lac amp N-CBP Thr BL21(DE3) ColE1 Stratagene expression vector
pET-3a map

seq

T7 amp none none BL21(DE3) pBR322 Novagen expression vector
pET-3b map

seq

T7 amp none none BL21(DE3) pBR322 Novagen expression vector
pET-3c map

seq

T7 amp none none BL21(DE3) pBR322 Novagen expression vector
pET-3d map

seq

T7 amp none none BL21(DE3) pBR322 Novagen expression vector formerly pET-8c
pET-12a map

seq

T7 amp leader seq. none BL21(DE3) pBR322 Novagen expression vector for secreted proteins
pET-14b map

seq

T7 amp N-His Thr BL21(DE3) pBR322 Novagen expression vector
pET-15b map

seq

T7/lac amp N-His Thr BL21(DE3) pBR322 Novagen expression vector
pET-16b map

seq

T7/lac amp N-His Xa BL21(DE3) pBR322 Novagen expression vector
pET-19b map

seq

T7/lac amp N-His Ent BL21(DE3) pBR322 Novagen expression vector
pET-20b(+) map

seq

T7 amp leader seq.

C-His

none BL21(DE3) pBR322 Novagen expression vector for secreted proteins
pET-21d(+) map

seq

T7/lac amp C-His none BL21(DE3) pBR322 Novagen expression vector
pET-22b(+) map

seq

T7/lac amp leader seq.
C-His
none BL21(DE3) pBR322 Novagen expression vector for secreted proteins
pET-24d(+) map

seq

T7/lac kan C-His none BL21(DE3) pBR322 Novagen expression vector
pET-28a map

seq

T7/lac kan N-His

C-His

Thr BL21(DE3) pBR322 Novagen expression vector
pET-28c map

seq

T7/lac kan N-His
C-His
Thr BL21(DE3) pBR322 Novagen expression vector
pET-32a(+) map

seq

T7/lac amp N-Trx
N-His
C-His
Thr
EK
AD494(DE3)
Origami (DE3)
pBR322 Novagen expression vector for disulfide bond containing proteins
pET-32b(+) map

seq

T7/lac amp N-Trx
N-His
C-His
Thr
EK
AD494(DE3)
Origami(DE3)
pBR322 Novagen expression vector for disulfide bond containing proteins
pET-32c(+) map

seq

T7/lac amp N-Trx
N-His
C-His
Thr
EK
AD494(DE3)
Origami(DE3)
pBR322 Novagen expression vector for disulfide bond containing proteins
pET-39b(+) map

seq

T7/lac kan N-DsbA
C-His
Thr
EK
BL21(DE3) pBR322 Novagen vector for expression in periplasm
pET-40b(+) map

seq

T7/lac kan N-DsbC
N-His
C-His
Thr
EK
BL21(DE3) pBR322 Novagen vector for expression in periplasm
pETM-10 map

seq

T7/lac kan H-His
C-His
none BL21(DE3) pBR322 G. Stier contains EFH insert in modified pET-24d
pETM-11 map

seq

T7/lac kan N-His TEV BL21(DE3) pBR322 G. Stier contains MAD insert in modified pET-24d
pETM11-SUMO3GFP map

seq

T7/lac kan N-His

N-SUMO3

SUMO BL21(DE3) pBR322 H. Besir pETM11 with E. coli optimized synthetic sumo3 gene
pETM-12 map

seq

T7/lac kan N-His Thr BL21(DE3) pBR322 G. Stier contains ABD insert in modified pET-9d
pETM-13 map

seq

T7/lac kan none none BL21(DE3) pBR322 G. Stier contains ABD insert in modified pET-24d
pETM-14 map

seq

T7/lac kan N-His 3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-14_ccdB map

seq

T7/lac kan N-His 3C BL21(DE3) pBR322 S. Suppmann (MPI Biochemistry) pETM-14 modified for SLIC
pETM-20 map

seq

T7/lac amp N-Trx
N-His
TEV BL21(DE3) pBR322 G. Stier contains RAGE insert in modified pET-32
pETM-21 map

seq

T7/lac amp N-Trx
N-His
Thr BL21(DE3) pBR322 G. Stier contains RAGE insert in modified pET-32
pETM-22 map

seq

T7/lac kan N-Trx
N-His
3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-22_ccdB map

seq

T7/lac kan

N-Trx

N-His

3C BL21(DE3) pBR322 S. Suppmann (MPI Biochemistry) pETM-22 modified for SLIC
pETM-30 map

seq

T7/lac kan N-His
N-GST
TEV BL21(DE3) pBR322 G. Stier contains DCOH insert in modified pET-24d
pETM-33 map

seq

T7/lac kan N-His
N-GST
3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-33_ccdB map

seq

T7/lac kan N-His

N-GST

3C BL21(DE3) pBR322 S. Suppmann (MPI Biochemistry) pETM-33 modified for SLIC
pETM-40 map

seq

T7/lac kan N-MBP TEV BL21(DE3) pBR322 G. Stier modified pET-24d; contains two STOP codons in MCS
pETM-41 map

seq

T7 kan N-His
N-MBP
TEV BL21(DE3) pBR322 G. Stier modified pET-9d; contains two STOP codons in MCS
pETM-43 map

seq

T7/lac kan N-MBP 3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-44 map

seq

T7/lac kan N-His

N-MBP

3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-44_ccdB map

seq

T7/lac kan N-His

N-MBP

3C BL21(DE3) pBR322 S. Suppmann (MPI Biochemistry) pETM-44 modified for SLIC
pETM-50 map

seq

T7/lac kan N-DsbA
N-His
TEV BL21(DE3) pBR322 G. Stier contains RAGE insert in modified pET-24d; leader sequence for export to periplasm
pETM-51 map

seq

T7/lac kan N-DsbA
N-His
Thr BL21(DE3) pBR322 G. Stier contains RAGE insert in modified pET-24d; leader sequence for export to periplasm
pETM-52 map

seq

T7/lac kan LL-DsbA
N-His
TEV BL21(DE3) pBR322 G. Stier contains RAGE insert in modified pET-24d; leaderless (LL) DsbA fusion for cytoplasmic expression
pETM-54 map

seq

T7/lac kan N-DsbA
N-His
3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; leader sequence for export to periplasm; contains two STOP codons in MCS
pETM-55 map

seq

T7/lac kan LL-DsbA
N-His
3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; leaderless (LL) DsbA fusion for cytoplasmic expression; contains two STOP codons in MCS
pETM-60 map

seq

T7/lac kan N-NusA
N-His
TEV BL21(DE3) pBR322 G. Stier contains ABD insert in modified pET-24d
pETM-66 map

seq

T7/lac kan N-NusA
N-His
3C BL21(DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-70 map

seq

T7/lac kan N-CBP TEV BL21(DE3) pBR322 A. Geerlof modified pET-24d
pETM-80 map

seq

T7/lac kan N-DsbC
N-His
TEV BL21(DE3) pBR322 G. Stier modified pET-24d; leader sequence for export to periplasm
pETM-82 map

seq

T7 kan LL-DsbC
N-His
TEV BL21(DE3) pBR322 G. Stier modified pET-9d; leaderless (LL) DsbC fusion for cytoplasmic expression
pGAT map

seq

T7 amp N-His
N-GST
Thr BL21(DE3) pUC M. Hyvönen expression vector
pGAT2 map

seq

T7 amp N-His
N-GST
Thr BL21(DE3) pUC M. Hyvönen expression vector
pGEX-3X map

seq

tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-4T-1 map

seq

tac amp N-GST Thr BL21 pBR322 Pharmacia expression vector
pGEX-4T-2 map

seq

tac amp N-GST Thr BL21 pBR322 Pharmacia expression vector
pGEX-4T-3 map

seq

tac amp N-GST Thr BL21 pBR322 Pharmacia expression vector
pGEX-5X-1 map

seq

tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-5X-2 map

seq

tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-5X-3 map

seq

tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-6P-1 map

seq

tac amp N-GST Pre BL21 pBR322 Pharmacia expression vector
pGEX-6P-2 map

seq

tac amp N-GST Pre BL21 pBR322 Pharmacia expression vector
pGEX-6P-3 map

seq

tac amp N-GST Pre BL21 pBR322 Pharmacia expression vector
pHAT map

seq

T7lac amp N-His none BL21(DE3) pUC M. Hyvönen expression vector
pHAT2 map

seq

T7lac amp N-His none BL21(DE3) pUC M. Hyvönen expression vector
pKK223-3 map

seq

tac amp none none lacIq
containing
strains eg.
JM105
pBR322 Pharmacia expression vector
pKK223-2 map

seq

trc amp none none        Pharmacia expression vector
pMal-c2 map

seq

tac amp N-MBP Xa TB1 or
any lacZa comple-
menting
strain
pBR322 New England Biolabs expression vector
pMal-p2 map

seq

tac amp leader seq.
N-MBP
Xa TB1 or
any lacZa comple-
menting
strain
pBR322 New England Biolabs vector for expression in periplasm
pProEx HTa map

seq

trc amp N-His TEV BL21
Life Technologies expression vector
pProEx HTb map

seq

trc amp N-His TEV BL21
Life Technologies expression vector
pProEx HTc map

seq

trc amp N-His TEV BL21
Life Technologies expression vector
pQE-16 map

seq

T5/lac amp N-DHFR

C-His

none M15[pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-30 map

seq

T5/lac amp N-His none M15[pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-31 map

seq

T5/lac amp N-His none M15[pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-32 map

seq

T5/lac amp N-His none M15[pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-60 map

seq

T5/lac amp C-His none M15[pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-70 map

seq

T5/lac amp C-His none M15[pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-80L map

seq

T5/lac amp N-His none M15 ColE1 Qiagen expression vector also expressing lac I
pQE-81L map

seq

T5/lac amp N-His none M15 ColE1 Qiagen expression vector also expressing lac I
pQE-82L map

seq

T5/lac amp N-His none M15 ColE1 Qiagen expression vector also expressing lac I
pRSET A map

seq

T7 amp N-His EK BL21(DE3)-

pLysS

ColE1 Invitrogen expression vector
pRSET B map

seq

T7 amp N-His EK BL21(DE3)-

pLysS

ColE1 Invitrogen expression vector
pRSET C map

seq

T7 amp N-His EK BL21(DE3)-

pLysS

ColE1 Invitrogen expression vector
pTrcHis2 A map

seq

trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pTrcHis2 B map

seq

trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pTrcHis2 C map

seq

trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pTrcHis2/
LacZ
map

seq

trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pZA31-Luc map

seq

PLtetO-1 cam none none DH5aZ1 p15A H. Bujard contains luciferase gene
pZE12-Luc map

seq

PLlacO-1 amp none none DH5aZ1 ColE1 H. Bujard contains luciferase gene
pZE21-MCS-1 map

seq

PLtetO-1 kan none none DH5aZ1 ColE1 H. Bujard high copy number expression vector
pZS*24-MCS-1 map

seq

Plac/ara-1 kan none none DH5aZ1 pSC*101 H. Bujard low copy number expression vector