High resolution studies of protein plasticity
For the study of biological mechanisms, protein engineering has become an essential tool. With genetically encoding non natural amino acids as our central strategy, we recruit a large plethora of novel chemical biology techniques to micro-manipulate biological function. A particular focus is the introduction of small, minimally perturbing fluorescent probes site selectively into proteins to study molecular structure and dynamics.
General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule Fluorescence Resonance Energy Transfer. Brustard EM, Lemke EA, Schultz PG, Deniz AA, A, J Am Chem Soc, 52,17664-5 (2008)
Control of protein phosphorylation with a genetically encoded photocaged amino acid. Lemke EA, Summerer D, Geierstanger B, Brittain S, Schultz PG, Nature Chem Biol 3, 769-772 (2007)
Single-molecule biophysics: at the interface of biology, physics and chemistry. Deniz AA, Mukhopadhyay S, Lemke EA, J R Soc Interface , 5(18), 15-45 (2008)