Functional dynamics of nuclear structure during the cell cycle
Engineering and physics are essential in two areas of our work.
1. To identify and characterize cell division genes systematically, we need novel high throughput methods.
In our group we:
- constantly engineer both software and hardware to automate advanced microscopy and single molecule methods and,
- apply machine vision approaches to increase throughput and decrease bias in these experiments.
2. In order to interpret protein dynamics inside cells we need to understand their rheology. To this end we are interested in the physical properties of the microenvironment in the cell nucleus, which we probe by biophysical methods and model in computer simulations.
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Neumann, B., Walter, T., Heriche, J.K., Bulkescher, J., Erfle, H., Conrad, C., Rogers, P., Poser, I., Held, M., Liebel, U., Cetin, C., Sieckmann, F., Pau, G., Kabbe, R., Wünsche, A., Satagopam, V., Schmitz, M.H., Chapuis, C., Gerlich, D.W., Schneider, R., Eils, R., Huber, W., Peters, J.M., Hyman, A.A., Durbin, R., Pepperkok, R. & Ellenberg, J. Nature. 2010 Apr 1;464(7289):721-7.
Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin. Bancaud, A., Huet, S., Daigle, N., Mozziconacci, J., Beaudouin, J. & Ellenberg, J. EMBO J. 2009 Dec 16;28(24):3785-98
Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins. Beaudouin, J., Mora-Bermudez, F., Klee, T., Daigle, N. & Ellenberg, J. Biophys J. 2006 Mar 15;90(6):1878-94