Left: diffraction limited image of Cdc11 at the mother-bud neck of budding yeast. Right: Three-dimensional superresolution reconstruction obtained with interference PALM. Scale bars: 500 nm.
Click on the figure for a larger version.
In order to investigate biological questions that were previously inaccessible because of the limited resolution of light microscopes, we are developing novel superresolution microscopy tools. The design and implementation of advanced microscopes is crucially based on physics and engineering such as optics, mechanical and electrical engineering, image analysis and software development.
Currently we are developing the following tools:
- Automated localization microscope for high-throughput superresolution imaging of entire proteomes.
- Robust three-dimensional superresolution microscopy with isotropic resolution.
- Superresolution microscopy with single-plane illumination.
- Microfluidics for superresolution microscopy.
Superresolution Imaging of Amyloid Fibrils with Binding-Activated Probes.
Ries, J., Udayar, V., Soragni, A., Hornemann, S., Nilsson, K.P., Riek, R., Hock, C., Ewers, H., Aguzzi, A.A. & Rajendran, L.
ACS Chem Neurosci. 2013 Apr 22. Europe PMC
A simple, versatile method for GFP-based super-resolution microscopy via nanobodies.
Ries, J., Kaplan, C., Platonova, E., Eghlidi, H. & Ewers, H.
Nat Methods. 2012 Jun;9(6):582-4. doi: 10.1038/nmeth.1991. Epub 2012 Apr 29. Europe PMC
Binding-activated localization microscopy of DNA structures.
Schoen, I., Ries, J., Klotzsch, E., Ewers, H. & Vogel, V.
Nano Lett. 2011 Sep 14;11(9):4008-11. Epub 2011 Aug 18. Europe PMC