Figure 1: 3D super-resolution microscopy allows dissecting the molecular organization of chromosome axes (green/cyan) and the synaptonemal complex (magenta/purple) that assembles between two homologous chromosomes (gray) within the germline of C. elegans.
+++ At EMBL from June 2018 +++
The Köhler group studies how chromatin is organized during meiosis to allow for the production of haploid gametes from diploid precursor cells.
Previous and current research
Meiosis is a specialized cell division process that generates haploid gametes from diploid precursor cells. During the meiotic cell division process, homologous chromosomes undergo recombination and are then segregated from each other. Errors in this process are a major cause for infertility and can also result in developmental disorders such as Down Syndrome. For homologous chromosomes to segregate properly during meiotic divisions, they must first pair during meiotic prophase. Pairing is ultimately stabilized by crossover formation, which generates physical linkages between the homologous chromosomes and gives rise to genetic diversity. However, how these processes are regulated remains unclear. More than a century ago using electron microscopy, scientists have identified a unique, ladder-like structure, the synaptonemal complex, which assembles between homologous chromosomes during meiosis. Since then, several studies have identified the components within this complex and have revealed that this structure is key in regulating meiotic processes.
Despite its wide conservation and its central role in meiosis, little is known about the molecular organization of the synaptonemal complex. Taking advantage of genome editing and single-molecule localization microscopy, we have recently revealed the three-dimensional architecture of this complex in the model organism C. elegans (Fig. 1). The model its molecular organization gives unique insight into how the synaptonemal complex might contribute to the genome wide regulation of crossover formation.
Future projects and goals
We now focus on the mechanisms that govern the formation of the synaptonemal complex and its role in chromatin organization. In particular, we are interested in the role of the synaptonemal complex in the regulation of crossover formation. We will investigate how the synaptonemal complex may limit the number and distribution of crossover recombination events. To address this question, we will take advantage of next generation sequencing, genome editing and state-of-the art imaging techniques.