EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
Biography
PhD 1992, University Kaiserslautern.
Postdoctoral research at University of Geneva.
Lab head at the Imperial Cancer Research Fund, London.
At EMBL since 1998.
Senior scientist since 2012.
Head of Core Facilities and Scientific Services since 2014.
Group leader in the Molecular Medicine Partnership Unit.
Publications
Total: 189 publication(s)
2021
Tischer C, Ravindran A, Reither S, Chiaruttini N, Pepperkok R, Norlin N. (2021)
BigDataProcessor2: a free and open-source Fiji plugin for inspection and processing of TB sized image data.
Bioinformatics doi: 10.1093/bioinformatics/btab106
Europe PMC | doi
2020
Khan MM, Poeckel D, Halavatyi A, Zukowska-Kasprzyk J, Stein F, Vappiani J, Sevin DC, Tischer C, Zinn N, Eley JD, Gudmann NS, Muley T, Winter H, Fisher AJ, Nanthakumar CB, Bergamini G, Pepperkok R. (2020)
An integrated multiomic and quantitative label-free microscopy-based approach to study pro-fibrotic signalling in ex vivo human precision-cut lung slices.
Eur Respir J doi: 10.1183/13993003.00221-2020
Europe PMC | doi
2019
Matos AM, Pinto FR, Barros P, Amaral MD, Pepperkok R, Matos P. (2019)
Inhibition of calpain 1 restores plasma membrane stability to pharmacologically rescued Phe508del-CFTR variant.
J. Biol. Chem. 294(36):13396-13410. doi: 10.1074/jbc.RA119.008738
Europe PMC | doi
Lippens S, D'Enfert C, Farkas L, Kehres A, Korn B, Morales M, Pepperkok R, Premvardhan L, Schlapbach R, Tiran A, Meder D, Van Minnebruggen G. (2019)
One step ahead: Innovation in core facilities.
EMBO Rep. 20(4) doi: 10.15252/embr.201948017
Europe PMC | doi
Giacomello E, Ronchi P, Pepperkok R. (2019)
GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.
J. Cell. Sci. 132(2) doi: 10.1242/jcs.222083
Europe PMC | doi
2018
Lérias JR, Pinto MC, Botelho HM, Awatade NT, Quaresma MC, Silva IAL, Wanitchakool P, Schreiber R, Pepperkok R, Kunzelmann K, Amaral MD. (2018)
A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic.
Biochim. Biophys. Acta 1865(2) doi: 10.1016/j.bbamcr.2017.11.009
Europe PMC | doi
2017
Colin S, Coelho LP, Sunagawa S, Bowler C, Karsenti E, Bork P, Pepperkok R, de Vargas C. (2017)
Quantitative 3D-imaging for cell biology and ecology of environmental microbial eukaryotes.
Elife 6 doi: 10.7554/eLife.26066
Europe PMC | doi
Kumar V, Fleming T, Terjung S, Gorzelanny C, Gebhardt C, Agrawal R, Mall MA, Ranzinger J, Zeier M, Madhusudhan T, Ranjan S, Isermann B, Liesz A, Deshpande D, Häring HU, Biswas SK, Reynolds PR, Hammes HP, Peperkok R, Angel P, Herzig S, Nawroth PP. (2017)
Homeostatic nuclear RAGE-ATM interaction is essential for efficient DNA repair.
Nucleic Acids Res. 45(18):10595-10613. doi: 10.1093/nar/gkx705
Europe PMC | doi
Gayathri Vegesna NV, Ronchi P, Durdu S, Terjung S, Pepperkok R. (2017)
Targeted Ablation Using Laser Nanosurgery.
Methods Mol. Biol. 1563:107-125. doi: 10.1007/978-1-4939-6810-7_8
Europe PMC | doi
2016
S Pedersen R, Karemore G, Gudjonsson T, Rask MB, Neumann B, Hériché JK, Pepperkok R, Ellenberg J, Gerlich DW, Lukas J, Lukas C. (2016)
Profiling DNA damage response following mitotic perturbations.
Nat Commun 7(13887):1-12. doi: 10.1038/ncomms13887
Europe PMC | doi
Hufnagel L, Pepperkok R. (2016)
Let there be light-at the right place.
Nat. Biotechnol. 34(12):1246-1247. doi: 10.1038/nbt.3738
Europe PMC | doi
Scharaw S, Iskar M, Ori A, Boncompain G, Laketa V, Poser I, Lundberg E, Perez F, Beck M, Bork P, Pepperkok R. (2016)
The endosomal transcriptional regulator RNF11 integrates degradation and transport of EGFR.
J. Cell Biol. 215(4):543-558. doi: 10.1083/jcb.201601090
Europe PMC | doi
Meder D, Morales M, Pepperkok R, Schlapbach R, Tiran A, Van Minnebruggen G. (2016)
Institutional core facilities: prerequisite for breakthroughs in the life sciences: Core facilities play an increasingly important role in biomedical research by providing scientists access to sophisticated technology and expertise.
EMBO Rep. 17(8):1088-1093. doi: 10.15252/embr.201642857
Europe PMC | doi
2015
Verissimo F, Halavatyi A, Pepperkok R, Weiss M. (2015)
A microtubule-independent role of p150glued in secretory cargo concentration at endoplasmic reticulum exit sites.
J Cell Sci 128(22) doi: 10.1242/jcs.172395
Europe PMC | doi
Frei DM, Hodneland E, Rios-Mondragon I, Burtey A, Neumann B, Bulkescher J, Schölermann J, Pepperkok R, Gerdes HH, Kögel T. (2015)
Erratum: Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer.
Sci Rep 5:14158. doi: 10.1038/srep14158
Europe PMC | doi
Frei DM, Hodneland E, Rios-Mondragon I, Burtey A, Neumann B, Bulkescher J, Schölermann J, Pepperkok R, Gerdes HH, Kögel T. (2015)
Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer.
Sci Rep 5:12879. doi: 10.1038/srep12879
Europe PMC | doi
Schuberth CE, Tangemo C, Coneva C, Tischer C, Pepperkok R. (2015)
Self-organization of core Golgi material is independent of COPII-mediated endoplasmic reticulum export
J. Cell Sci. 128(7):1279-1293. doi: 10.1242/jcs.154443
Europe PMC | doi
Wachsmuth M, Conrad C, Bulkescher J, Koch B, Mahen R, Isokane M, Pepperkok R, Ellenberg J. (2015)
High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells.
Nat. Biotechnol. 33(4):384-389. doi: 10.1038/nbt.3146
Europe PMC | doi
Lecat S, Matthes HW, Pepperkok R, Simpson JC, Galzi JL. (2015)
A fluorescent live imaging screening assay based on translocation criteria identifies novel cytoplasmic proteins implicated in GPCR signalling pathways.
Mol. Cell Proteomics 14(5) doi: 10.1074/mcp.m114.046698
Europe PMC | doi
Botelho HM, Uliyakina I, Awatade NT, Proença MC, Tischer C, Sirianant L, Kunzelmann K, Pepperkok R, Amaral MD. (2015)
Protein traffic disorders: an effective high-throughput fluorescence microscopy pipeline for drug discovery.
Sci Rep 5:9038. doi: 10.1038/srep09038
Europe PMC | doi
Thormaehlen AS, Schuberth C, Won HH, Blattmann P, Joggerst-Thomalla B, Theiss S, Asselta R, Duga S, Merlini PA, Ardissino D, Lander ES, Gabriel S, Rader DJ, Peloso GM, Pepperkok R, Kathiresan S, Runz H. (2015)
Systematic Cell-Based Phenotyping of Missense Alleles Empowers Rare Variant Association Studies: A Case for LDLR and Myocardial Infarction.
PLoS Genet. 11(2) doi: 10.1371/journal.pgen.1004855
Europe PMC | doi
Zacherl S, La Venuta G, Müller HM, Wegehingel S, Dimou E, Sehr P, Lewis JD, Erfle H, Pepperkok R, Nickel W. (2015)
A direct role for ATP1A1 in unconventional secretion of fibroblast growth factor 2.
J. Biol. Chem. 290(6):3654-3665. doi: 10.1074/jbc.m114.590067
Europe PMC | doi
2014
Ronchi P, Tischer C, Acehan D, Pepperkok R. (2014)
Positive feedback between golgi membranes, microtubules and ER-exit sites directs golgi de novo biogenesis.
J. Cell. Sci. 127(21):4620-4633. doi: 10.1242/jcs.150474
Europe PMC | doi
Tischer C, Hilsenstein V, Hanson K, Pepperkok R. (2014)
Adaptive fluorescence microscopy by online feedback image analysis.
Methods Cell Biol. 123:489-503. doi: 10.1016/b978-0-12-420138-5.00026-4
Europe PMC | doi
Eskova A, Knapp B, Matelska D, Reusing S, Arjonen A, Lisauskas T, Pepperkok R, Russell R, Eils R, Ivaska J, Kaderali L, Erfle H, Starkuviene V. (2014)
An RNAi screen identifies KIF15 as a novel regulator of the endocytic trafficking of integrin.
J. Cell. Sci. 127(11):2433-2447. doi: 10.1242/jcs.137281
Europe PMC | doi
Sauvageau E, Rochdi MD, Oueslati M, Hamdan FF, Percherancier Y, Simpson JC, Pepperkok R, Bouvier M. (2014)
CNIH4 interacts with newly synthesized GPCR and controls their export from the endoplasmic reticulum.
Traffic 15(4):383-400. doi: 10.1111/tra.12148
Europe PMC | doi
Michel G, Matthes HW, Hachet-Haas M, El Baghdadi K, de Mey J, Pepperkok R, Simpson JC, Galzi JL, Lecat S. (2014)
Plasma membrane translocation of REDD1 governed by GPCRs contributes to mTORC1 activation
J. Cell. Sci. 127(4):773-787. doi: 10.1242/jcs.136432
Europe PMC | doi
Laketa V, Zarbakhsh S, Traynor-Kaplan A, Macnamara A, Subramanian D, Putyrski M, Mueller R, Nadler A, Mentel M, Saez-Rodriguez J, Pepperkok R, Schultz C. (2014)
PIP₃ induces the recycling of receptor tyrosine kinases
Sci Signal 7(308):ra5. doi: 10.1126/scisignal.2004532
Europe PMC | doi
2013
Verissimo F, Pepperkok R. (2013)
Imaging ER-to-Golgi transport: towards a systems view
J. Cell. Sci. 126(Pt 22):5091-5100. doi: 10.1242/jcs.121061
Europe PMC | doi
Botelho HM, Dahimene S, Uliyakina I, Neumann B, Tischer C, Pepperkok R, Amaral MD. (2013)
HIGH THROUGHPUT SCREEN REVEALS GENES REGULATING WT-CFTR AND RESCUING F508DEL-CFTR TRAFFIC
PEDIATR PULM 48:208.
He S, Ni D, Ma B, Lee JH, Zhang T, Ghozalli I, Pirooz SD, Zhao Z, Bharatham N, Li B, Oh S, Lee WH, Takahashi Y, Wang HG, Minassian A, Feng P, Deretic V, Pepperkok R, Tagaya M, Yoon HS, Liang C. (2013)
PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential interactions with the ER tether and the beclin 1 complex
Nat. Cell Biol. 15(10):1206-1219. doi: 10.1038/ncb2848
Europe PMC | doi
Almaça J, Faria D, Sousa M, Uliyakina I, Conrad C, Sirianant L, Clarke LA, Martins JP, Santos M, Heriché JK, Huber W, Schreiber R, Pepperkok R, Kunzelmann K, Amaral MD. (2013)
High-content siRNA screen reveals global ENaC regulators and potential cystic fibrosis therapy targets
Cell 154(6):1390-1400. doi: 10.1016/j.cell.2013.08.045
Europe PMC | doi
Gerdes HH, Pepperkok R. (2013)
Cell-to-cell communication: current views and future perspectives
Cell Tissue Res. 352(1):1-3. doi: 10.1007/s00441-013-1590-1
Europe PMC | doi
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E. (2013)
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Nat. Methods 10(4):315-323. doi: 10.1038/nmeth.2377
Europe PMC | doi
Blattmann P, Schuberth C, Pepperkok R, Runz H. (2013)
RNAi-based functional profiling of loci from blood lipid genome-wide association studies identifies genes with cholesterol-regulatory function
PLoS Genet. 9(2):e1003338. doi: 10.1371/journal.pgen.1003338
Europe PMC | doi
Iskar M, Zeller G, Blattmann P, Campillos M, Kuhn M, Kaminska KH, Runz H, Gavin AC, Pepperkok R, van Noort V, Bork P. (2013)
Characterization of drug-induced transcriptional modules: towards drug repositioning and functional understanding
Mol. Syst. Biol. 9:662. doi: 10.1038/msb.2013.20
Europe PMC | doi
Ronchi P, Pepperkok R. (2013)
Golgi depletion from living cells with laser nanosurgery
Methods Cell Biol. 118:311-324. doi: 10.1016/b978-0-12-417164-0.00019-7
Europe PMC | doi
2012
Simpson JC, Joggerst B, Laketa V, Verissimo F, Cetin C, Erfle H, Bexiga MG, Singan VR, Hériché JK, Neumann B, Mateos A, Blake J, Bechtel S, Benes V, Wiemann S, Ellenberg J, Pepperkok R. (2012)
Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway
Nat. Cell Biol. 14(7):764-774. doi: 10.1038/ncb2510
Europe PMC | doi
Moudry P, Lukas C, Macurek L, Neumann B, Heriche JK, Pepperkok R, Ellenberg J, Hodny Z, Lukas J, Bartek J. (2012)
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1.
Cell Death Differ. 19(5):798-807. doi: 10.1038/cdd.2011.150
Europe PMC | doi
Ronchi P, Terjung S, Pepperkok R. (2012)
At the cutting edge: applications and perspectives of laser nanosurgery in cell biology
Biol. Chem. 393(4):235-248. doi: 10.1515/hsz-2011-0237
Europe PMC | doi
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E. (2012)
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
J Proteomics 75(7):2236-2251. doi: 10.1016/j.jprot.2012.01.030
Europe PMC | doi
Taverna E, Haffner C, Pepperkok R, Huttner WB. (2012)
A new approach to manipulate the fate of single neural stem cells in tissue
Nat. Neurosci. 15(2):329-337. doi: 10.1038/nn.3008
Europe PMC | doi
Contreras FX, Ernst AM, Haberkant P, Björkholm P, Lindahl E, Gönen B, Tischer C, Elofsson A, von Heijne G, Thiele C, Pepperkok R, Wieland F, Brügger B. (2012)
Molecular recognition of a single sphingolipid species by a protein's transmembrane domain
Nature 481(7382):525-529. doi: 10.1038/nature10742
Europe PMC | doi
Roth SD, Schüttrumpf J, Milanov P, Abriss D, Ungerer C, Quade-Lyssy P, Simpson JC, Pepperkok R, Seifried E, Tonn T. (2012)
Chemical chaperones improve protein secretion and rescue mutant factor VIII in mice with hemophilia A
PLoS ONE 7(9):e44505. doi: 10.1371/journal.pone.0044505
Europe PMC | doi
Seitz A, Terjung S, Zimmermann T, Pepperkok R. (2012)
Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching-based fluorescence resonance energy transfer measurements.
J Biomed Opt 17(1):011010. doi: 10.1117/1.jbo.17.1.011010
Europe PMC | doi
2011
Ritzerfeld J, Remmele S, Wang T, Temmerman K, Brügger B, Wegehingel S, Tournaviti S, Strating JR, Wieland FT, Neumann B, Ellenberg J, Lawerenz C, Hesser J, Erfle H, Pepperkok R, Nickel W. (2011)
Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases
Genome Res. 21(11):1955-1968. doi: 10.1101/gr.116087.110
Europe PMC | doi
Moore HM, Bai B, Boisvert FM, Latonen L, Rantanen V, Simpson JC, Pepperkok R, Lamond AI, Laiho M. (2011)
Quantitative proteomics and dynamic imaging of the nucleolus reveal distinct responses to UV and ionizing radiation
Mol. Cell Proteomics 10(10):M111.009241. doi: 10.1074/mcp.m111.009241
Europe PMC | doi
Conrad C, Wünsche A, Tan TH, Bulkescher J, Sieckmann F, Verissimo F, Edelstein A, Walter T, Liebel U, Pepperkok R, Ellenberg J. (2011)
Micropilot: automation of fluorescence microscopy-based imaging for systems biology
Nat. Methods 8(3):246-249. doi: 10.1038/nmeth.1558
Europe PMC | doi
Tängemo C, Ronchi P, Colombelli J, Haselmann U, Simpson JC, Antony C, Stelzer EH, Pepperkok R, Reynaud EG. (2011)
A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells
J. Cell. Sci. 124(Pt 6):978-987. doi: 10.1242/jcs.079640
Europe PMC | doi
Almaça J, Dahimène S, Appel N, Conrad C, Kunzelmann K, Pepperkok R, Amaral MD. (2011)
Functional genomics assays to study CFTR traffic and ENaC function
Methods Mol. Biol. 742:249-264. doi: 10.1007/978-1-61779-120-8_15
Europe PMC | doi
Reiss S, Rebhan I, Backes P, Romero-Brey I, Erfle H, Matula P, Kaderali L, Poenisch M, Blankenburg H, Hiet MS, Longerich T, Diehl S, Ramirez F, Balla T, Rohr K, Kaul A, Bühler S, Pepperkok R, Lengauer T, Albrecht M, Eils R, Schirmacher P, Lohmann V, Bartenschlager R. (2011)
Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.
Cell Host Microbe 9(1):32-45. doi: 10.1016/j.chom.2010.12.002
Europe PMC | doi
2010
Terjung S, Walter T, Seitz A, Neumann B, Pepperkok R, Ellenberg J. (2010)
High-throughput microscopy using live mammalian cells.
Cold Spring Harb Protoc 2010(7):pdb.top84. doi: 10.1101/pdb.top84
Europe PMC | doi
Ebert AD, Laussmann M, Wegehingel S, Kaderali L, Erfle H, Reichert J, Lechner J, Beer HD, Pepperkok R, Nickel W. (2010)
Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion
Traffic 11(6):813-826. doi: 10.1111/j.1600-0854.2010.01059.x
Europe PMC | doi
Grecco HE, Roda-Navarro P, Girod A, Hou J, Frahm T, Truxius DC, Pepperkok R, Squire A, Bastiaens PI. (2010)
In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays
Nat. Methods 7(6):467-472. doi: 10.1038/nmeth.1458
Europe PMC | doi
Subramanian D, Laketa V, Müller R, Tischer C, Zarbakhsh S, Pepperkok R, Schultz C. (2010)
Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells
Nat. Chem. Biol. 6(5):324-326. doi: 10.1038/nchembio.348
Europe PMC | doi
Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J. (2010)
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
Nature 464(7289):721-727. doi: 10.1038/nature08869
Europe PMC | doi
Walter T, Held M, Neumann B, Hériché JK, Conrad C, Pepperkok R, Ellenberg J. (2010)
Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging
J. Struct. Biol. 170(1):1-9. doi: 10.1016/j.jsb.2009.10.004
Europe PMC | doi
Börner K, Hermle J, Sommer C, Brown NP, Knapp B, Glass B, Kunkel J, Torralba G, Reymann J, Beil N, Beneke J, Pepperkok R, Schneider R, Ludwig T, Hausmann M, Hamprecht F, Erfle H, Kaderali L, Kräusslich HG, Lehmann MJ. (2010)
From experimental setup to bioinformatics: an RNAi screening platform to identify host factors involved in HIV-1 replication
Biotechnol J 5(1):39-49. doi: 10.1002/biot.200900226
Europe PMC | doi
2009
Heinz S, Schüttrumpf J, Simpson JC, Pepperkok R, Nicolaes GA, Abriss D, Milanov P, Roth S, Seifried E, Tonn T. (2009)
Factor VIII-eGFP fusion proteins with preserved functional activity for the analysis of the early secretory pathway of factor VIII
Thromb. Haemost. 102(5):925-935. doi: 10.1160/th08-12-0807
Europe PMC | doi
Laketa V, Zarbakhsh S, Morbier E, Subramanian D, Dinkel C, Brumbaugh J, Zimmermann P, Pepperkok R, Schultz C. (2009)
Membrane-permeant phosphoinositide derivatives as modulators of growth factor signaling and neurite outgrowth
Chem. Biol. 16(11):1190-1196. doi: 10.1016/j.chembiol.2009.10.005
Europe PMC | doi
Gilbert DF, Meinhof T, Pepperkok R, Runz H. (2009)
DetecTiff: a novel image analysis routine for high-content screening microscopy
J Biomol Screen 14(8):944-955. doi: 10.1177/1087057109339523
Europe PMC | doi
Schwarz K, Iolascon A, Verissimo F, Trede NS, Horsley W, Chen W, Paw BH, Hopfner KP, Holzmann K, Russo R, Esposito MR, Spano D, De Falco L, Heinrich K, Joggerst B, Rojewski MT, Perrotta S, Denecke J, Pannicke U, Delaunay J, Pepperkok R, Heimpel H. (2009)
Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II
Nat. Genet. 41(8):936-940. doi: 10.1038/ng.405
Europe PMC | doi
Tournaviti S, Pietro ES, Terjung S, Schafmeier T, Wegehingel S, Ritzerfeld J, Schulz J, Smith DF, Pepperkok R, Nickel W. (2009)
Reversible phosphorylation as a molecular switch to regulate plasma membrane targeting of acylated SH4 domain proteins
Traffic 10(8):1047-1060. doi: 10.1111/j.1600-0854.2009.00921.x
Europe PMC | doi
Bartz F, Kern L, Erz D, Zhu M, Gilbert D, Meinhof T, Wirkner U, Erfle H, Muckenthaler M, Pepperkok R, Runz H. (2009)
Identification of cholesterol-regulating genes by targeted RNAi screening
Cell Metab. 10(1):63-75. doi: 10.1016/j.cmet.2009.05.009
Europe PMC | doi
Martinez-Morales JR, Rembold M, Greger K, Simpson JC, Brown KE, Quiring R, Pepperkok R, Martin-Bermudo MD, Himmelbauer H, Wittbrodt J. (2009)
ojoplano-mediated basal constriction is essential for optic cup morphogenesis
Development 136(13):2165-2175. doi: 10.1242/dev.033563
Europe PMC | doi
Doil C, Mailand N, Bekker-Jensen S, Menard P, Larsen DH, Pepperkok R, Ellenberg J, Panier S, Durocher D, Bartek J, Lukas J, Lukas C. (2009)
RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins
Cell 136(3):435-446. doi: 10.1016/j.cell.2008.12.041
Europe PMC | doi
2008
Dejgaard SY, Murshid A, Erman A, Kizilay O, Verbich D, Lodge R, Dejgaard K, Ly-Hartig TB, Pepperkok R, Simpson JC, Presley JF. (2008)
Rab18 and Rab43 have key roles in ER-Golgi trafficking
J. Cell. Sci. 121(Pt 16):2768-2781. doi: 10.1242/jcs.021808
Europe PMC | doi
Erfle H, Neumann B, Rogers P, Bulkescher J, Ellenberg J, Pepperkok R. (2008)
Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates
J Biomol Screen 13(7):575-580. doi: 10.1177/1087057108320133
Europe PMC | doi
Pouthas F, Girard P, Lecaudey V, Ly TB, Gilmour D, Boulin C, Pepperkok R, Reynaud EG. (2008)
In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum
J. Cell. Sci. 121(Pt 14):2406-2414. doi: 10.1242/jcs.026849
Europe PMC | doi
Mathivanan S, Ahmed M, Ahn NG, Alexandre H, Amanchy R, Andrews PC, Bader JS, Balgley BM, Bantscheff M, Bennett KL, Björling E, Blagoev B, Bose R, Brahmachari SK, Burlingame AS, Bustelo XR, Cagney G, Cantin GT, Cardasis HL, Celis JE, Chaerkady R, Chu F, Cole PA, Costello CE, Cotter RJ, Crockett D, DeLany JP, De Marzo AM, DeSouza LV, Deutsch EW, Dransfield E, Drewes G, Droit A, Dunn MJ, Elenitoba-Johnson K, Ewing RM, Van Eyk J, Faca V, Falkner J, Fang X, Fenselau C, Figeys D, Gagné P, Gelfi C, Gevaert K, Gimble JM, Gnad F, Goel R, Gromov P, Hanash SM, Hancock WS, Harsha HC, Hart G, Hays F, He F, Hebbar P, Helsens K, Hermeking H, Hide W, Hjernø K, Hochstrasser DF, Hofmann O, Horn DM, Hruban RH, Ibarrola N, James P, Jensen ON, Jensen PH, Jung P, Kandasamy K, Kheterpal I, Kikuno RF, Korf U, Körner R, Kuster B, Kwon MS, Lee HJ, Lee YJ, Lefevre M, Lehvaslaiho M, Lescuyer P, Levander F, Lim MS, Löbke C, Loo JA, Mann M, Martens L, Martinez-Heredia J, McComb M, McRedmond J, Mehrle A, Menon R, Miller CA, Mischak H, Mohan SS, Mohmood R, Molina H, Moran MF, Morgan JD, Moritz R, Morzel M, Muddiman DC, Nalli A, Navarro JD, Neubert TA, Ohara O, Oliva R, Omenn GS, Oyama M, Paik YK, Pennington K, Pepperkok R, Periaswamy B, Petricoin EF, Poirier GG, Prasad TS, Purvine SO, Rahiman BA, Ramachandran P, Ramachandra YL, Rice RH, Rick J, Ronnholm RH, Salonen J, Sanchez JC, Sayd T, Seshi B, Shankari K, Sheng SJ, Shetty V, Shivakumar K, Simpson RJ, Sirdeshmukh R, Siu KW, Smith JC, Smith RD, States DJ, Sugano S, Sullivan M, Superti-Furga G, Takatalo M, Thongboonkerd V, Trinidad JC, Uhlen M, Vandekerckhove J, Vasilescu J, Veenstra TD, Vidal-Taboada JM, Vihinen M, Wait R, Wang X, Wiemann S, Wu B, Xu T, Yates JR, Zhong J, Zhou M, Zhu Y, Zurbig P, Pandey A. (2008)
Human Proteinpedia enables sharing of human protein data
Nat. Biotechnol. 26(2):164-167. doi: 10.1038/nbt0208-164
Europe PMC | doi
Colombelli J, Tängemo C, Haselman U, Antony C, Stelzer EH, Pepperkok R, Reynaud EG. (2008)
A correlative light and electron microscopy method based on laser micropatterning and etching
Methods Mol. Biol. 457:203-213. doi: 10.1007/978-1-59745-261-8_15
Europe PMC | doi
Pepperkok R, Wiemann S. (2008)
Integrating systems biology with clinical research
Genome Biol. 9(7):314. doi: 10.1186/gb-2008-9-7-314
Europe PMC | doi
Verissimo F, Pepperkok R. (2008)
ER-to-Golgi transport
In: Mironov AA & Pavelka M (eds.), The Golgi Apparatus: State of the art 110 years after Camillo Golgi's discovery. Springer Vienna. pp 333-341.doi: 10.1007/978-3-211-76310-0_20
doi
Walter T, Held M, Neumann B, Heriche JK, Conrad C, Pepperkok R, Ellenberg J. (2008)
A genome wide RNAI screen by time lapse microscopy in order to identify mitotic genes - Computational aspects and challenges.
Paper presented at the 2008 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Paris, France, 14-17 May 2008.I S Biomed Imaging 1:328-331. doi: 10.1109/ISBI.2008.4540999
doi
2007
Starkuviene V, Pepperkok R. (2007)
The potential of high-content high-throughput microscopy in drug discovery
Br. J. Pharmacol. 152(1):62-71. doi: 10.1038/sj.bjp.0707346
Europe PMC | doi
Starkuviene V, Pepperkok R. (2007)
Differential requirements for ts-O45-G and procollagen biosynthetic transport
Traffic 8(8):1035-1051. doi: 10.1111/j.1600-0854.2007.00582.x
Europe PMC | doi
Starkuviene V, Pepperkok R, Erfle H. (2007)
Transfected cell microarrays: an efficient tool for high-throughput functional analysis
Expert Rev Proteomics 4(4):479-489. doi: 10.1586/14789450.4.4.479
Europe PMC | doi
Simpson JC, Cetin C, Erfle H, Joggerst B, Liebel U, Ellenberg J, Pepperkok R. (2007)
An RNAi screening platform to identify secretion machinery in mammalian cells
Paper presented at the International Workshop on Molecular Systems Biology, Bielefeld, GERMANY, JUN, 2006.J. Biotechnol. 129(2):352-365. doi: 10.1016/j.jbiotec.2006.12.027
Europe PMC | doi
Erfle H, Neumann B, Liebel U, Rogers P, Held M, Walter T, Ellenberg J, Pepperkok R. (2007)
Reverse transfection on cell arrays for high content screening microscopy.
Nat Protoc 2(2):392-399. doi: 10.1038/nprot.2006.483
Europe PMC | doi
Erfle H, Pepperkok R. (2007)
Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells
Methods Mol. Biol. 360:155-161. doi: 10.1385/1-59745-165-7:155
Europe PMC | doi
Grabher C, Cliffe A, Miura K, Hayflick J, Pepperkok R, Rørth P, Wittbrodt J. (2007)
Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka
Paper presented at the EMBO Workshop on Innate Danger Signals and HMGB1, Milan, ITALY, FEB 08-11, 2006.J. Leukoc. Biol. 81(1):263-271. doi: 10.1189/jlb.0806526
Europe PMC | doi
Laketa V, Simpson JC, Bechtel S, Wiemann S, Pepperkok R. (2007)
High-content microscopy identifies new neurite outgrowth regulators
Mol. Biol. Cell 18(1):242-252. doi: 10.1091/mbc.e06-08-0666
Europe PMC | doi
Robinson DG, Herranz MC, Bubeck J, Pepperkok R, Ritzenthaler C. (2007)
Membrane dynamics in the early secretory pathway
CRC Crit Rev Plant Sci 26(4):199-225. doi: 10.1080/07352680701495820
Europe PMC | doi
Simpson JC, Mateos A, Pepperkok R. (2007)
Maturation of the mammalian secretome
Genome Biol. 8(4):211. doi: 10.1186/gb-2007-8-4-211
Europe PMC | doi
von Brunn A, Teepe C, Simpson JC, Pepperkok R, Friedel CC, Zimmer R, Roberts R, Baric R, Haas J. (2007)
Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome
PLoS ONE 2(5):e459. doi: 10.1371/journal.pone.0000459
Europe PMC | doi
2006
Stauber T, Simpson JC, Pepperkok R, Vernos I. (2006)
A role for kinesin-2 in COPI-dependent recycling between the ER and the Golgi complex
Curr. Biol. 16(22):2245-2251. doi: 10.1016/j.cub.2006.09.060
Europe PMC | doi
Brunet S, Zimmermann T, Reynaud EG, Vernos I, Karsenti E, Pepperkok R. (2006)
Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion.
Traffic 7(9):1283-1289. doi: 10.1111/j.1600-0854.2006.00463.x
Europe PMC | doi
Pepperkok R, Ellenberg J. (2006)
High-throughput fluorescence microscopy for systems biology
Nat. Rev. Mol. Cell Biol. 7(9):690-696. doi: 10.1038/nrm1979
Europe PMC | doi
Runz H, Miura K, Weiss M, Pepperkok R. (2006)
Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G
EMBO J. 25(13):2953-2965. doi: 10.1038/sj.emboj.7601205
Europe PMC | doi
Veríssimo F, Silva E, Morris JD, Pepperkok R, Jordan P. (2006)
Protein kinase WNK3 increases cell survival in a caspase-3-dependent pathway
Oncogene 25(30):4172-4182. doi: 10.1038/sj.onc.1209449
Europe PMC | doi
Neumann B, Held M, Liebel U, Erfle H, Rogers P, Pepperkok R, Ellenberg J. (2006)
High-throughput RNAi screening by time-lapse imaging of live human cells.
Nat. Methods 3(5):385-390. doi: 10.1038/nmeth876
Europe PMC | doi
Tabary O, Boncoeur E, de Martin R, Pepperkok R, Clément A, Schultz C, Jacquot J. (2006)
Calcium-dependent regulation of NF-(kappa)B activation in cystic fibrosis airway epithelial cells
Cell. Signal. 18(5):652-660. doi: 10.1016/j.cellsig.2005.06.004
Europe PMC | doi
Simpson JC, Nilsson T, Pepperkok R. (2006)
Biogenesis of tubular ER-to-Golgi transport intermediates
Mol. Biol. Cell 17(2):723-737. doi: 10.1091/mbc.e05-06-0580
Europe PMC | doi
Colombelli J, Pepperkok R, Stelzer EH, Reynaud EG. (2006)
[Laser nanosurgery in cell biology]
Med Sci (Paris) 22(6-7):651-658. doi: 10.1051/medsci/20062267651
Europe PMC | doi
Forster R, Weiss M, Zimmermann T, Reynaud EG, Verissimo F, Stephens DJ, Pepperkok R. (2006)
Secretory cargo regulates the turnover of COPII subunits at single ER exit sites.
Curr. Biol. 16(2):173-179. doi: 10.1016/j.cub.2005.11.076
Europe PMC | doi
Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S. (2006)
The LIFEdb database in 2006
Nucleic Acids Res. 34(Database issue):D415-8. doi: 10.1093/nar/gkj139
Europe PMC | doi
Neumann B, Held M, Liebel U, Erfle H, Rogers P, Pepperkok R, Ellenberg J. (2006)
Time-lapse microscopy-based genome wide RNAI screening in live human cells.
Paper presented at the 2006 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Arlington, VA (USA), 6-9 April 2006.I S Biomed Imaging 1:245-246. doi: 10.1109/ISBI.2006.1624898
doi
Simpson JC, Pepperkok R. (2006)
Systematic Subcellular Localization of Novel Proteins
Third Edition edn. Academic Press.doi: 10.1016/B978-012164730-8/50140-4
doi
Simpson JC, Pepperkok R. (2006)
The subcellular localization of the mammalian proteome comes a fraction closer
Genome Biol. 7(6):222. doi: 10.1186/gb-2006-7-6-222
Europe PMC | doi
2005
Colombelli J, Reynaud EG, Rietdorf J, Pepperkok R, Stelzer EH. (2005)
In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery
Traffic 6(12):1093-1102. doi: 10.1111/j.1600-0854.2005.00334.x
Europe PMC | doi