EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Biography

  • PhD 1992, University Kaiserslautern.
    Postdoctoral research at University of Geneva.
    Lab head at the Imperial Cancer Research Fund, London.
    At EMBL since 1998.
    Senior scientist since 2012.
    Head of Core Facilities and Scientific Services since 2014.

Publications

Total: 183 publication(s)

2005

Ben-Tekaya H, Miura K, Pepperkok R, Hauri HP. (2005)

Live imaging of bidirectional traffic from the ERGIC

J. Cell. Sci. 118(Pt 2):357-367. doi: 10.1242/jcs.01615
Europe PMC | doi

Erfle H, Pepperkok R. (2005)

Arrays of transfected mammalian cells for high content screening microscopy

Meth. Enzymol. 404:1-8. doi: 10.1016/s0076-6879(05)04001-2
Europe PMC | doi

Liebel U, Kindler B, Pepperkok R. (2005)

Bioinformatic "Harvester": a search engine for genome-wide human, mouse, and rat protein resources

Meth. Enzymol. 404:19-26. doi: 10.1016/s0076-6879(05)04003-6
Europe PMC | doi

Moss DA, Keese M, Pepperkok R. (2005)

IR micro spectroscopy of live cells

Vib Spectrosc 38(1-2):185-191.
Europe PMC

Pepperkok R, Simpson JC, Rietdorf J, Cetin C, Liebel U, Terjung S, Zimmermann T. (2005)

Imaging platforms for measurement of membrane trafficking

Meth. Enzymol. 404:8-18. doi: 10.1016/s0076-6879(05)04002-4
Europe PMC | doi

Reynaud EG, Andrade MA, Bonneau F, Ly TB, Knop M, Scheffzek K, Pepperkok R. (2005)

Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization

BMC Biol. 3:21. doi: 10.1186/1741-7007-3-21
Europe PMC | doi

Terjung S, Pepperkok R, Ankerhold R, Caiolfa VR, Castel S, Majer P, Geiger B, Luini A, Salamero J, Shorte S, Small JV. (2005)

EAMNET - a resource and teaching framework for advanced light microscopy

Eur. J. Cell Biol. 84:108.
Europe PMC

Watson P, Forster R, Palmer KJ, Pepperkok R, Stephens DJ. (2005)

Coupling of ER exit to microtubules through direct interaction of COPII with dynactin

Nat. Cell Biol. 7(1):48-55. doi: 10.1038/ncb1206
Europe PMC | doi

Young J, Stauber T, del Nery E, Vernos I, Pepperkok R, Nilsson T. (2005)

Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'

Mol. Biol. Cell 16(1):162-177. doi: 10.1091/mbc.e04-03-0260
Europe PMC | doi


2004

Brunet S, Sardon T, Zimmerman T, Wittmann T, Pepperkok R, Karsenti E, Vernos I. (2004)

Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts

Mol. Biol. Cell 15(12):5318-5328. doi: 10.1091/mbc.e04-05-0385
Europe PMC | doi

Starkuviene V, Liebel U, Simpson JC, Erfle H, Poustka A, Wiemann S, Pepperkok R. (2004)

High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic

Genome Res. 14(10A):1948-1956. doi: 10.1101/gr.2658304
Europe PMC | doi

Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A. (2004)

From ORFeome to biology: a functional genomics pipeline

Genome Res. 14(10B):2136-2144. doi: 10.1101/gr.2576704
Europe PMC | doi

Erfle H, Simpson JC, Bastiaens PI, Pepperkok R. (2004)

siRNA cell arrays for high-content screening microscopy

BioTechniques 37(3):454-8, 460, 462.
Europe PMC

Liebel U, Kindler B, Pepperkok R. (2004)

'Harvester': a fast meta search engine of human protein resources

Bioinformatics 20(12):1962-1963. doi: 10.1093/bioinformatics/bth146
Europe PMC | doi

Becker S, Simpson JC, Pepperkok R, Heinz S, Herder C, Grez M, Seifried E, Tonn T. (2004)

Confocal microscopy analysis of native, full length and B-domain deleted coagulation factor VIII trafficking in mammalian cells

Thromb. Haemost. 92(1):23-35. doi: 10.1267/thro04070023
Europe PMC | doi

Stephens DJ, Pepperkok R. (2004)

Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum

J. Cell. Sci. 117(Pt 16):3635-3644. doi: 10.1242/jcs.01269
Europe PMC | doi

Conrad C, Erfle H, Warnat P, Daigle N, Lörch T, Ellenberg J, Pepperkok R, Eils R. (2004)

Automatic identification of subcellular phenotypes on human cell arrays

Genome Res. 14(6):1130-1136. doi: 10.1101/gr.2383804
Europe PMC | doi

Bannasch D, Mehrle A, Glatting KH, Pepperkok R, Poustka A, Wiemann S. (2004)

LIFEdb: a database for functional genomics experiments integrating information from external sources, and serving as a sample tracking system

Nucleic Acids Res. 32(Database issue):D505-8. doi: 10.1093/nar/gkh022
Europe PMC | doi


2003

Liebel U, Starkuviene V, Erfle H, Simpson JC, Poustka A, Wiemann S, Pepperkok R. (2003)

A microscope-based screening platform for large-scale functional protein analysis in intact cells

FEBS Lett. 554(3):394-398. doi: 10.1016/s0014-5793(03)01197-9
Europe PMC | doi

Zimmermann T, Rietdorf J, Pepperkok R. (2003)

Spectral imaging and its applications in live cell microscopy

FEBS Lett. 546(1):87-92. doi: 10.1016/s0014-5793(03)00521-0
Europe PMC | doi

Simpson JC, Pepperkok R. (2003)

Localizing the proteome

Genome Biol. 4(12):240. doi: 10.1186/gb-2003-4-12-240
Europe PMC | doi

Wiemann S, Bechtel S, Bannasch D, Pepperkok R, Poustka A, German cDNA Network. (2003)

The German cDNA network: cDNAs, functional genomics and proteomics

J. Struct. Funct. Genomics 4(2-3):87-96. doi: 10.1023/a:1026148428520
Europe PMC | doi

Wiemann S, Mehrle A, Bechtel S, Wellenreuther R, Pepperkok R, Poustka A, German cDNA Consortium. (2003)

CDNAs for functional genomics and proteomics: the German Consortium

C. R. Biol. 326(10-11):1003-1009. doi: 10.1016/j.crvi.2003.09.036
Europe PMC | doi


2002

Gruss OJ, Wittmann M, Yokoyama H, Pepperkok R, Kufer T, Silljé H, Karsenti E, Mattaj IW, Vernos I. (2002)

Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells

Nat. Cell Biol. 4(11):871-879. doi: 10.1038/ncb870
Europe PMC | doi

Zimmermann T, Rietdorf J, Girod A, Georget V, Pepperkok R. (2002)

Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair

FEBS Lett. 531(2):245-249. doi: 10.1016/s0014-5793(02)03508-1
Europe PMC | doi

Richards AA, Stang E, Pepperkok R, Parton RG. (2002)

Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection

Mol. Biol. Cell 13(5):1750-1764. doi: 10.1091/mbc.01-12-0592
Europe PMC | doi

Runz H, Rietdorf J, Tomic I, de Bernard M, Beyreuther K, Pepperkok R, Hartmann T. (2002)

Inhibition of intracellular cholesterol transport alters presenilin localization and amyloid precursor protein processing in neuronal cells

J. Neurosci. 22(5):1679-1689.
Europe PMC

Stephens DJ, Pepperkok R. (2002)

Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells

J. Cell. Sci. 115(Pt 6):1149-1160.
Europe PMC

Liebel U, Pepperkok R. (2002)

A fully automated high content screening system for cell based genome analyses

Transkript Laborwelt 2002(1):4-8.


2001

Kerkhoff E, Simpson JC, Leberfinger CB, Otto IM, Doerks T, Bork P, Rapp UR, Raabe T, Pepperkok R. (2001)

The Spir actin organizers are involved in vesicle transport processes

Curr. Biol. 11(24):1963-1968. doi: 10.1016/s0960-9822(01)00602-9
Europe PMC | doi

Stephens DJ, Pepperkok R. (2001)

The many ways to cross the plasma membrane

Proc. Natl. Acad. Sci. U.S.A. 98(8):4295-4298. doi: 10.1073/pnas.081065198
Europe PMC | doi

Stephens DJ, Pepperkok R. (2001)

Illuminating the secretory pathway: when do we need vesicles?

J. Cell. Sci. 114(Pt 6):1053-1059.
Europe PMC

Pepperkok R, Simpson JC, Wiemann S. (2001)

Being in the right location at the right time

Genome Biol. 2(9):REVIEWS1024. doi: 10.1186/gb-2001-2-9-reviews1024
Europe PMC | doi

Simpson JC, Neubrand VE, Wiemann S, Pepperkok R. (2001)

Illuminating the human genome

Histochem. Cell Biol. 115(1):23-29. doi: 10.1007/s004180000236
Europe PMC | doi


2000

Bastiaens PI, Pepperkok R. (2000)

Observing proteins in their natural habitat: the living cell

Trends Biochem. Sci. 25(12):631-637. doi: 10.1016/s0968-0004(00)01714-x
Europe PMC | doi

Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S. (2000)

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing

EMBO Rep. 1(3):287-292. doi: 10.1093/embo-reports/kvd058
Europe PMC | doi

Storrie B, Pepperkok R, Nilsson T. (2000)

Breaking the COPI monopoly on Golgi recycling

Trends Cell Biol. 10(9):385-391. doi: 10.1016/s0962-8924(00)01818-3
Europe PMC | doi

Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP. (2000)

COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites

J. Cell. Sci. 113 ( Pt 12):2177-2185.
Europe PMC

Pepperkok R, Hotz-Wagenblatt A, König N, Girod A, Bossemeyer D, Kinzel V. (2000)

Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidation

J. Cell Biol. 148(4):715-726. doi: 10.1083/jcb.148.4.715
Europe PMC | doi

Denzel A, Otto F, Girod A, Pepperkok R, Watson R, Rosewell I, Bergeron JJ, Solari RC, Owen MJ. (2000)

The p24 family member p23 is required for early embryonic development

Curr. Biol. 10(1):55-58. doi: 10.1016/s0960-9822(99)00266-3
Europe PMC | doi

Pepperkok R, Girod A, Simpson J, Rietdorf J. (2000)

Immunofluorescence microscopy

Oxford University Press, Oxford.

Pepperkok R, Shima G. (2000)

Fluorescence microscopy of living vertebrate cells

Oxford Univ. Press.

Pepperkok R, Whitney JA, Gomez M, Kreis TE. (2000)

COPI vesicles accumulating in the presence of a GTP restricted arf1 mutant are depleted of anterograde and retrograde cargo

J. Cell. Sci. 113 ( Pt 1):135-144.
Europe PMC

Rietdorf J, Stephens DJ, Squire A, Simpson J, Shima DT, Paccaud JP, Bastiaens PIH, Pepperkok R. (2000)

Illuminating cellular structure and function in the early secretory pathway by multispectral 3D imaging in living cells

P SOC PHOTO-OPT INS 1(15):232-241.


1999

Heiss NS, Girod A, Salowsky R, Wiemann S, Pepperkok R, Poustka A. (1999)

Dyskerin localizes to the nucleolus and its mislocalization is unlikely to play a role in the pathogenesis of dyskeratosis congenita

Hum. Mol. Genet. 8(13):2515-2524. doi: 10.1093/hmg/8.13.2515
Europe PMC | doi

Girod A, Storrie B, Simpson JC, Johannes L, Goud B, Roberts LM, Lord JM, Nilsson T, Pepperkok R. (1999)

Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum

Nat. Cell Biol. 1(7):423-430. doi: 10.1038/15658
Europe PMC | doi

Pepperkok R. (1999)

New imaging methods illuminating cellular structure and function

EUR J CANCER 35:S66.

Gillham H, Golding MC, Pepperkok R, Gullick WJ. (1999)

Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling

J. Cell Biol. 146(4):869-880. doi: 10.1083/jcb.146.4.869
Europe PMC | doi

Pepperkok R, Squire A, Geley S, Bastiaens PI. (1999)

Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy

Curr. Biol. 9(5):269-272. doi: 10.1016/s0960-9822(99)80117-1
Europe PMC | doi

Jackson ME, Simpson JC, Girod A, Pepperkok R, Roberts LM, Lord JM. (1999)

The KDEL retrieval system is exploited by Pseudomonas exotoxin A, but not by Shiga-like toxin-1, during retrograde transport from the Golgi complex to the endoplasmic reticulum

J. Cell. Sci. 112 ( Pt 4):467-475.
Europe PMC

Shima DT, Scales SJ, Kreis TE, Pepperkok R. (1999)

Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes

Curr. Biol. 9(15):821-824. doi: 10.1016/s0960-9822(99)80365-0
Europe PMC | doi


1998

Majoul I, Sohn K, Wieland FT, Pepperkok R, Pizza M, Hillemann J, Söling HD. (1998)

KDEL receptor (Erd2p)-mediated retrograde transport of the cholera toxin A subunit from the Golgi involves COPI, p23, and the COOH terminus of Erd2p

J. Cell Biol. 143(3):601-612. doi: 10.1083/jcb.143.3.601
Europe PMC | doi

Pepperkok R, Lowe M, Burke B, Kreis TE. (1998)

Three distinct steps in transport of vesicular stomatitis virus glycoprotein from the ER to the cell surface in vivo with differential sensitivities to GTP gamma S

J. Cell. Sci. 111 ( Pt 13):1877-1888.
Europe PMC

Herr S, Pepperkok R, Saffrich R, Wiemann S and Ansorge W. (1998)

Electroporation of cells

Academic Press, London.

Pepperkok R, Rosorius O, Scheel J. (1998)

Cellular microbiochemistry

Springer Berlin Heidelberg.doi: 10.1007/978-3-642-80343-7_8
Europe PMC | doi

Pepperkok R, Saffrich R, Ansorge W. (1998)

Computer-automated and semiautomated capillary microinjection of macromolecules into living cells

In: Celis, JE (ed), Cell Biology: A Laboratory Handbook, vol 4. 2 edn. Academic Press. pp 23-30.


1997

Scales SJ, Pepperkok R, Kreis TE. (1997)

Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI

Cell 90(6):1137-1148. doi: 10.1016/s0092-8674(00)80379-7
Europe PMC | doi

Scheel J, Pepperkok R, Lowe M, Griffiths G, Kreis TE. (1997)

Dissociation of coatomer from membranes is required for brefeldin A-induced transfer of Golgi enzymes to the endoplasmic reticulum

J. Cell Biol. 137(2):319-333. doi: 10.1083/jcb.137.2.319
Europe PMC | doi


1996

Pepperkok R. (1996)

Microinjection and electroporation of macromolecules into live cells

In: M. Pagano (ed.), Springer Lab Manual Cell Cycle-Materials and Methods. Springer Berlin Heidelberg. pp 75-86.doi: 10.1007/978-3-642-57783-3_7
Europe PMC | doi


1995

Griffiths G, Pepperkok R, Locker JK, Kreis TE. (1995)

Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN

J. Cell. Sci. 108 ( Pt 8):2839-2856.
Europe PMC

PEPPERKOK R, ANSORGE W. (1995)

DIRECT VISUALIZATION OF DNA-REPLICATION SITES IN LIVING CELLS BY MICROINJECTION OF FLUORESCEIN-CONJUGATED DUTPS

METHOD MOL CELL BIOL 5(2):112-117.


1994

PEVERALI FA, RAMQVIST T, SAFFRICH R, PEPPERKOK R, BARONE MV, PHILIPSON L. (1994)

REGULATION OF G(1) PROGRESSION BY E2A AND ID HELIX-LOOP-HELIX PROTEINS

EMBO J 13(18):4291-4301.
Europe PMC

Peverali FA, Ramqvist T, Saffrich R, Pepperkok R, Barone MV, Philipson L. (1994)

Regulation of G1 progression by E2A and Id helix-loop-helix proteins

EMBO J. 13(18):4291-4301.
Europe PMC

Barone MV, Pepperkok R, Peverali FA, Philipson L. (1994)

Id proteins control growth induction in mammalian cells

Proc. Natl. Acad. Sci. U.S.A. 91(11):4985-4988. doi: 10.1073/pnas.91.11.4985
Europe PMC | doi

Storrie B, Pepperkok R, Stelzer EH, Kreis TE. (1994)

The intracellular mobility of a viral membrane glycoprotein measured by confocal microscope fluorescence recovery after photobleaching

J. Cell. Sci. 107 ( Pt 5):1309-1319.
Europe PMC

PEPPERKOK R, LORENZ P, ANSORGE W, PYERIN W. (1994)

CASEIN KINASE-II IS REQUIRED FOR TRANSITION OF G(0)/G(1), EARLY G(1), AND G(1)/S PHASES OF THE CELL-CYCLE

J BIOL CHEM 269(9):6986-6991.
Europe PMC


1993

Twamley-Stein GM, Pepperkok R, Ansorge W, Courtneidge SA. (1993)

The Src family tyrosine kinases are required for platelet-derived growth factor-mediated signal transduction in NIH 3T3 cells

Proc. Natl. Acad. Sci. U.S.A. 90(16):7696-7700. doi: 10.1073/pnas.90.16.7696
Europe PMC | doi

Pepperkok R, Scheel J, Horstmann H, Hauri HP, Griffiths G, Kreis TE. (1993)

Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo

Cell 74(1):71-82. doi: 10.1016/0092-8674(93)90295-2
Europe PMC | doi

Pagano M, Pepperkok R, Lukas J, Baldin V, Ansorge W, Bartek J, Draetta G. (1993)

Regulation of the cell cycle by the cdk2 protein kinase in cultured human fibroblasts

J. Cell Biol. 121(1):101-111. doi: 10.1083/jcb.121.1.101
Europe PMC | doi

Soto U, Pepperkok R, Ansorge W, Just WW. (1993)

Import of firefly luciferase into mammalian peroxisomes in vivo requires nucleoside triphosphates

Exp. Cell Res. 205(1):66-75. doi: 10.1006/excr.1993.1059
Europe PMC | doi

Pepperkok R, Herr S, Lorenz P, Pyerin W, Ansorge W. (1993)

System for quantitation of gene expression in single cells by computerized microimaging: application to c-fos expression after microinjection of anti-casein kinase II antibody

Exp. Cell Res. 204(2):278-285. doi: 10.1006/excr.1993.1034
Europe PMC | doi

Duden R, Storrie B, Pepperkok R, Scheel J, Joggerst-Thomalla B, Sawyer A, Horstmann H, Griffiths G, Kreis T. (1993)

β-COP, a Coat Protein of Nonclathrin-Coated Vesicles of the Golgi Complex, is Involved in Transport of Vesicular Stomatitis Virus Glycoprotein

In: Morré DJ, Howell KE, Bergeron JJM (eds.), Molecular Mechanisms of Membrane Traffic, vol 74. NATO ASI Series. Springer Berlin Heidelberg. pp 117-126.doi: 10.1007/978-3-662-02928-2_26
doi

Herr S, Bastian T, Pepperkok R, Boulin C, Ansorge W. (1993)

A fully automated image acquisition and analysis system for low light level fluorescence microscopy

Methods Mol. Cell. Biol. 40:164-170.

Scheel J, Rickard J, Pierre P, Hennig D, Karecla P, Pepperkok R, Joggerst-Thomalla B, Sawyer A, Parton R, Kreis T. (1993)

CLIP-170, a Cytoplasmic Linker Protein Mediating Interaction of Endosomes with Microtubules

In: Morré DJ, Howell KE, Bergeron JJM (eds.), Molecular Mechanisms of Membrane Traffic, vol 74. NATO ASI Series. Springer Berlin Heidelberg. pp 145-157.doi: 10.1007/978-3-662-02928-2_29
doi


1992

Pyerin W, Pepperkok R, Ansorge W, Lorenz P. (1992)

Early cell growth stimulation is inhibited by casein kinase II antisense oligodeoxynucleotides

Ann. N. Y. Acad. Sci. 660:295-297. doi: 10.1111/j.1749-6632.1992.tb21091.x
Europe PMC | doi


1991

Carmo-Fonseca M, Tollervey D, Pepperkok R, Barabino SM, Merdes A, Brunner C, Zamore PD, Green MR, Hurt E, Lamond AI. (1991)

Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery

EMBO J. 10(1):195-206.
Europe PMC

Pepperkok R, Schneider C, Philipson L, Ansorge W. (1991)

Single cell assay with an automated capillary microinjection system

Cytotechnology 5 Suppl 1:93-98. doi: 10.1007/bf00736821
Europe PMC | doi


1990

Bré MH, Pepperkok R, Hill AM, Levilliers N, Ansorge W, Stelzer EH, Karsenti E. (1990)

Regulation of microtubule dynamics and nucleation during polarization in MDCK II cells

J. Cell Biol. 111(6 Pt 2):3013-3021. doi: 10.1083/jcb.111.6.3013
Europe PMC | doi

Sorrentino V, Pepperkok R, Davis RL, Ansorge W, Philipson L. (1990)

Cell proliferation inhibited by MyoD1 independently of myogenic differentiation

Nature 345(6278):813-815. doi: 10.1038/345813a0
Europe PMC | doi


1989

Rosa P, Weiss U, Pepperkok R, Ansorge W, Niehrs C, Stelzer EH, Huttner WB. (1989)

An antibody against secretogranin I (chromogranin B) is packaged into secretory granules

J. Cell Biol. 109(1):17-34. doi: 10.1083/jcb.109.1.17
Europe PMC | doi

Ansorge W, Voss H, Wirkner U, Schwager C, Stegemann J, Pepperkok R, Zimmermann J, Erfle H. (1989)

Automated Sanger DNA sequencing with one label in less than four lanes on gel

J. Biochem. Biophys. Methods 20(1):47-52. doi: 10.1016/0165-022x(89)90080-8
Europe PMC | doi


1988

Pepperkok R, Zanetti M, King R, Delia D, Ansorge W, Philipson L, Schneider C. (1988)

Automatic microinjection system facilitates detection of growth inhibitory mRNA

Proc. Natl. Acad. Sci. U.S.A. 85(18):6748-6752. doi: 10.1073/pnas.85.18.6748
Europe PMC | doi

Ansorge W, Pepperkok R. (1988)

Performance of an automated system for capillary microinjection into living cells

J. Biochem. Biophys. Methods 16(4):283-292. doi: 10.1016/0165-022x(88)90062-0
Europe PMC | doi