EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Phone: +49 6221 387-8199


  • PhD 2008, TU Dresden.
    Postdoctoral research research at the ETH Zurich.
    Group Leader at EMBL Heidelberg since 2012.
    ERC Investigator.


Total: 65 publication(s)


Thevathasan JV, Kahnwald M, Cieśliński K, Hoess P, Peneti SK, Reitberger M, Heid D, Kasuba KC, Hoerner SJ, Li Y, Wu YL, Mund M, Matti U, Pereira PM, Henriques R, Kueblbeck M, Nijmeijer B, Ellenberg J, Sabinina VJ, Ries J. (2019)

Nuclear pores as versatile reference standards for quantitative superresolution microscopy.

Nat. Methods 16(10):1045-1053. doi: 10.1038/s41592-019-0574-9
Europe PMC | doi

Schlichthaerle T, Strauss MT, Schueder F, Auer A, Nijmeijer B, Kueblbeck M, Sabinina VJ, Thevathasan JV, Ries J, Ellenberg J, Jungmann R. (2019)

Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA-PAINT.

Angew. Chem. Int. Ed. Engl. 58(37):13004-13008. doi: 10.1002/anie.201905685
Europe PMC | doi

Manenschijn HE, Picco A, Mund M, Rivier-Cordey AS, Ries J, Kaksonen M. (2019)

Type-I myosins promote actin polymerization to drive membrane bending in endocytosis.

Elife 8 doi: 10.7554/eLife.44215
Europe PMC | doi

Li Y, Wu YL, Hoess P, Mund M, Ries J. (2019)

Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization.

Biomed Opt Express 10(6) doi: 10.1364/BOE.10.002708
Europe PMC | doi

Levet F, Julien G, Galland R, Butler C, Beghin A, Chazeau A, Hoess P, Ries J, Giannone G, Sibarita JB. (2019)

A tessellation-based colocalization analysis approach for single-molecule localization microscopy.

Nat Commun 10(1) doi: 10.1038/s41467-019-10007-4
Europe PMC | doi

Sage D, Pham TA, Babcock H, Lukes T, Pengo T, Chao J, Velmurugan R, Herbert A, Agrawal A, Colabrese S, Wheeler A, Archetti A, Rieger B, Ober R, Hagen GM, Ries J, Sibarita JB, Unser M, Henriques R, Holden S. (2019)

Publisher Correction: Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software.

Nat Methods 16(6) doi: 10.1038/s41592-019-0453-4
Europe PMC | doi

Sage D, Pham TA, Babcock H, Lukes T, Pengo T, Chao J, Velmurugan R, Herbert A, Agrawal A, Colabrese S, Wheeler A, Archetti A, Rieger B, Ober R, Hagen GM, Sibarita JB, Ries J, Henriques R, Unser M, Holden S.. (2019)

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software.

Nat. Methods 16(5) doi: 10.1038/s41592-019-0364-4
Europe PMC | doi


Unsay JD, Murad F, Hermann E, Ries J, García-Sáez AJ. (2018)

Scanning Fluorescence Correlation Spectroscopy for Quantification of the Dynamics and Interactions in Tube Organelles of Living Cells.

Chemphyschem 19(23) doi: 10.1002/cphc.201800705
Europe PMC | doi

Schlichthaerle T, Eklund A, Schueder F, Strauss M, Tiede C, Curd A, Ries J, Peckham M, Tomlinson D, Jungmann R. (2018)

Site-specific labeling of Affimers for DNA-PAINT microscopy.

Angew Chem Int Ed Engl 57(34) doi: 10.1002/anie.201804020
Europe PMC | doi

Mund M, van der Beek JA, Deschamps J, Dmitrieff S, Hoess P, Monster JL, Picco A, Nédélec F, Kaksonen M, Ries J. (2018)

Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation.

Cell 174(4) doi: 10.1016/j.cell.2018.06.032
Europe PMC | doi

Li Y, Mund M, Hoess P, Deschamps J, Matti U, Nijmeijer B, Sabinina VJ, Ellenberg J, Schoen I, Ries J. (2018)

Real-time 3D single-molecule localization using experimental point spread functions.

Nat Methods 15(5) doi: 10.1038/nmeth.4661
Europe PMC | doi

Hoess P, Mund M, Reitberger M, Ries J. (2018)

Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies.

Methods Mol Biol 1764:237-251. doi: 10.1007/978-1-4939-7759-8_14
Europe PMC | doi

Barton C, Morganella S, Ødegård-Fougner Ø, Alexander S, Ries J, Fitzgerald T, Ellenberg J, Birney E. (2018)

ChromoTrace: Computational reconstruction of 3D chromosome configurations for super-resolution microscopy.

PLoS Comput Biol 14(3) doi: 10.1371/journal.pcbi.1006002
Europe PMC | doi


Schoen I, Aires L, Ries J, Vogel V. (2017)

Nanoscale invaginations of the nuclear envelope: Shedding new light on wormholes with elusive function.

Nucleus 8(5):506-514. doi: 10.1080/19491034.2017.1337621
Europe PMC | doi

Shrestha RL, Conti D, Tamura N, Braun D, Ramalingam RA, Cieslinski K, Ries J, Draviam VM. (2017)

Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.

Nat Commun 8(1) doi: 10.1038/s41467-017-00209-z
Europe PMC | doi

Hauke S, von Appen A, Quidwai T, Ries J, Wombacher R. (2017)

Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells.

Chem Sci 8(1):559-566. doi: 10.1039/C6SC02088G
Europe PMC | doi


Morley SJ, Qi YM, Iovino L, Andolfi L, Guo D, Kalebic N, Castaldi L, Tischer C, Portulano C, Bolasco G, Shirlekar K, Fusco CM, Asaro A, Fermani F, Sundukova M, Matti U, Reymond L, De Ninno A, Businaro L, Johnsson K, Lazzarino M, Ries J, Schwab Y, Hu J, Heppenstall PA. (2016)

Acetylated tubulin is essential for touch sensation in mice

ELIFE 5 doi: 10.7554/eLife.20813
Europe PMC | doi

Deschamps J, Rowald A, Ries J. (2016)

Efficient homogeneous illumination and optical sectioning for quantitative single-molecule localization microscopy.

Opt Express 24(24):28080-28090. doi: 10.1364/oe.24.028080
Europe PMC | doi

Salvador-Gallego R, Mund M, Cosentino K, Schneider J, Unsay J, Schraermeyer U, Engelhardt J, Ries J, García-Sáez AJ. (2016)

Bax assembly into rings and arcs in apoptotic mitochondria is linked to membrane pores.

EMBO J. 35(4) doi: 10.15252/embj.201593384
Europe PMC | doi


Früh SM, Schoen I, Ries J, Vogel V. (2015)

Molecular architecture of native fibronectin fibrils.

Nat Commun 6 doi: 10.1038/ncomms8275
Europe PMC | doi

Kaplan C, Jing B, Winterflood CM, Bridges AA, Occhipinti P, Schmied J, Grinhagens S, Gronemeyer T, Tinnefeld P, Gladfelter AS, Ries J, Ewers H. (2015)

Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.

Nano Lett. 15(6):3859-3864. doi: 10.1021/acs.nanolett.5b00693
Europe PMC | doi

Picco A, Mund M, Ries J, Nédélec F, Kaksonen M. (2015)

Visualizing the functional architecture of the endocytic machinery.

Elife 4 doi: 10.7554/eLife.04535
Europe PMC | doi

Hermann E, Ries J, García-Sáez AJ. (2015)

Scanning fluorescence correlation spectroscopy on biomembranes.

Methods Mol. Biol. 1232:181-197. doi: 10.1007/978-1-4939-1752-5_15
Europe PMC | doi


Deschamps J, Mund M, Ries J. (2014)

3D superresolution microscopy by supercritical angle detection.

Opt Express 22(23):29081-29091. doi: 10.1364/oe.22.029081
Europe PMC | doi

Mund M, Kaplan C, Ries J. (2014)

Localization microscopy in yeast

Methods Cell Biol. 123:253-271. doi: 10.1016/b978-0-12-420138-5.00014-8
Europe PMC | doi

Cieśliński K, Ries J. (2014)

The yeast kinetochore - structural insights from optical microscopy

Curr Opin Chem Biol 20C:1-8. doi: 10.1016/j.cbpa.2014.03.020
Europe PMC | doi

Anderluh A, Klotzsch E, Ries J, Reismann AW, Weber S, Fölser M, Koban F, Freissmuth M, Sitte HH, Schütz GJ. (2014)

Tracking single serotonin transporter molecules at the endoplasmic reticulum and plasma membrane

Biophys. J. 106(9):L33-5. doi: 10.1016/j.bpj.2014.03.019
Europe PMC | doi

Klotzsch E, Schoen I, Ries J, Renn A, Sandoghdar V, Vogel V. (2014)

Conformational distribution of surface-adsorbed fibronectin molecules explored by single molecule localization microscopy

BIOMATER SCI-UK 2(6):883-892. doi: 10.1039/c3bm60262a


Udayar V, Buggia-Prévot V, Guerreiro RL, Siegel G, Rambabu N, Soohoo AL, Ponnusamy M, Siegenthaler B, Bali J, AESG, Simons M, Ries J, Puthenveedu MA, Hardy J, Thinakaran G, Rajendran L. (2013)

A paired RNAi and RabGAP overexpression screen identifies Rab11 as a regulator of β-amyloid production

Cell Rep 5(6):1536-1551. doi: 10.1016/j.celrep.2013.12.005
Europe PMC | doi

Ries J, Udayar V, Soragni A, Hornemann S, Nilsson KP, Riek R, Hock C, Ewers H, Aguzzi AA, Rajendran L. (2013)

Superresolution imaging of amyloid fibrils with binding-activated probes

ACS Chem Neurosci 4(7):1057-1061. doi: 10.1021/cn400091m
Europe PMC | doi

Apraiz I, Campbel R, Ries J, Riddell A, Cole R. (2013)

Workshop Session Abstracts

J Biomol Tech 24(Suppl):S19.
Europe PMC

Kleine Borgmann LA, Ries J, Ewers H, Ulbrich MH, Graumann PL. (2013)

The bacterial SMC complex displays two distinct modes of interaction with the chromosome

Cell Rep 3(5):1483-1492. doi: 10.1016/j.celrep.2013.04.005
Europe PMC | doi

Schoen I, Kaplan C, Platonova E, Vogel V, Ewers H, Ries J. (2013)

Novel Labeling Schemes for Single-Molecule Nanoscopy.

Paper presented at the ABRF 13: “Tools For the Advancement of Convergence Science”, Palm Springs, California, 2-5 March 2013.J Biomol Tech 24(Suppl):S7.
Europe PMC


Schmidt C, Kim B, Grabner H, Ries J, Kulomaa M, Vogel V. (2012)

Tuning the "roadblock" effect in kinesin-based transport

Nano Lett. 12(7):3466-3471. doi: 10.1021/nl300936j
Europe PMC | doi

Ries J, Kaplan C, Platonova E, Eghlidi H, Ewers H. (2012)

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

Nat. Methods 9(6):582-584. doi: 10.1038/nmeth.1991
Europe PMC | doi

Ries J, Schwille P. (2012)

Fluorescence correlation spectroscopy

Bioessays 34(5):361-368. doi: 10.1002/bies.201100111
Europe PMC | doi

Ries J, Weidemann T, Schwille P. (2012)

Fluorescence correlation spectroscopy

Elsevier.doi: 10.1016/b978-0-12-374920-8.00219-8
Europe PMC | doi


Schoen I, Ries J, Klotzsch E, Ewers H, Vogel V. (2011)

Binding-activated localization microscopy of DNA structures

Nano Lett. 11(9):4008-4011. doi: 10.1021/nl2025954
Europe PMC | doi

Boldajipour B, Doitsidou M, Tarbashevich K, Laguri C, Yu SR, Ries J, Dumstrei K, Thelen S, Dörries J, Messerschmidt EM, Thelen M, Schwille P, Brand M, Lortat-Jacob H, Raz E. (2011)

Cxcl12 evolution--subfunctionalization of a ligand through altered interaction with the chemokine receptor

Development 138(14):2909-2914. doi: 10.1242/dev.068379
Europe PMC | doi

Schwille P, Ries J. (2011)

Principles and Applications of Fluorescence Correlation Spectroscopy (FCS)

In: Bartolo BD & Collins J (eds.), Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer Netherlands. pp 63-85.doi: 10.1007/978-90-481-9977-8_4
Europe PMC | doi


Jeyasekharan AD, Ayoub N, Mahen R, Ries J, Esposito A, Rajendra E, Hattori H, Kulkarni RP, Venkitaraman AR. (2010)

DNA damage regulates the mobility of Brca2 within the nucleoplasm of living cells

Proc. Natl. Acad. Sci. U.S.A. 107(50):21937-21942. doi: 10.1073/pnas.1009577107
Europe PMC | doi

Ries J, Bayer M, Csúcs G, Dirkx R, Solimena M, Ewers H, Schwille P. (2010)

Automated suppression of sample-related artifacts in Fluorescence Correlation Spectroscopy

Opt Express 18(11):11073-11082. doi: 10.1364/oe.18.011073
Europe PMC | doi

Petrásek Z, Ries J, Schwille P. (2010)

Scanning FCS for the characterization of protein dynamics in live cells

Meth. Enzymol. 472:317-343. doi: 10.1016/s0076-6879(10)72005-x
Europe PMC | doi

Ries J, Petrasek Z, Garcia-Saez AJ, Schwille P. (2010)

A comprehensive framework for fluorescence cross-correlation spectroscopy

New J Phys 12:113009.
Europe PMC


García-Sáez AJ, Ries J, Orzáez M, Pérez-Payà E, Schwille P. (2009)

Membrane promotes tBID interaction with BCL(XL)

Nat. Struct. Mol. Biol. 16(11):1178-1185. doi: 10.1038/nsmb.1671
Europe PMC | doi

Ivashyna O, García-Sáez AJ, Ries J, Christenson ET, Schwille P, Schlesinger PH. (2009)

Detergent-activated BAX protein is a monomer

J. Biol. Chem. 284(36):23935-23946. doi: 10.1074/jbc.m109.023853
Europe PMC | doi

Ries J, Yu SR, Burkhardt M, Brand M, Schwille P. (2009)

Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms

Nat. Methods 6(9):643-645. doi: 10.1038/nmeth.1355
Europe PMC | doi

Yu SR, Burkhardt M, Nowak M, Ries J, Petrásek Z, Scholpp S, Schwille P, Brand M. (2009)

Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules

Nature 461(7263):533-536. doi: 10.1038/nature08391
Europe PMC | doi

Ries J, Chiantia S, Schwille P. (2009)

Accurate determination of membrane dynamics with line-scan FCS

Biophys. J. 96(5):1999-2008. doi: 10.1016/j.bpj.2008.12.3888
Europe PMC | doi

Chiantia S, Ries J, Schwille P. (2009)

Fluorescence correlation spectroscopy in membrane structure elucidation

Biochim. Biophys. Acta 1788(1):225-233. doi: 10.1016/j.bbamem.2008.08.013
Europe PMC | doi


Lingwood D, Ries J, Schwille P, Simons K. (2008)

Plasma membranes are poised for activation of raft phase coalescence at physiological temperature

Proc. Natl. Acad. Sci. U.S.A. 105(29):10005-10010. doi: 10.1073/pnas.0804374105
Europe PMC | doi

Ries J, Petrov EP, Schwille P. (2008)

Total internal reflection fluorescence correlation spectroscopy: effects of lateral diffusion and surface-generated fluorescence

Biophys. J. 95(1):390-399. doi: 10.1529/biophysj.107.126193
Europe PMC | doi

Ries J, Schwille P. (2008)

New concepts for fluorescence correlation spectroscopy on membranes

Phys Chem Chem Phys 10(24):3487-3497. doi: 10.1039/b718132a
Europe PMC | doi

Chiantia S, Ries J, Chwastek G, Carrer D, Li Z, Bittman R, Schwille P. (2008)

Role of ceramide in membrane protein organization investigated by combined AFM and FCS

Biochim. Biophys. Acta 1778(5):1356-1364. doi: 10.1016/j.bbamem.2008.02.008
Europe PMC | doi

Loose M, Fischer-Friedrich E, Ries J, Kruse K, Schwille P. (2008)

Spatial regulators for bacterial cell division self-organize into surface waves in vitro

Science 320(5877):789-792. doi: 10.1126/science.1154413
Europe PMC | doi

Rajendran L, Schneider A, Schlechtingen G, Weidlich S, Ries J, Braxmeier T, Schwille P, Schulz JB, Schroeder C, Simons M, Jennings G, Knolker H, Simons K. (2008)

Efficient Inhibition of the Alzheimer's Disease  -Secretase by Membrane Targeting

Science 320(5875) doi: 10.1126/science.1156609
Europe PMC | doi

Ries J, Klose C, Walch-Solimena C, Schwille P. (2008)

How to measure slow diffusion in yeast cell membranes

doi: 10.1117/12.787043

Ries J, Ruckstuhl T, Verdes D, Schwille P. (2008)

Supercritical angle fluorescence correlation spectroscopy

Biophys. J. 94(1):221-229. doi: 10.1529/biophysj.107.115998
Europe PMC | doi


Kippert A, Trajkovic K, Rajendran L, Ries J, Simons M. (2007)

Rho regulates membrane transport in the endocytic pathway to control plasma membrane specialization in oligodendroglial cells

J. Neurosci. 27(13):3560-3570. doi: 10.1523/jneurosci.4926-06.2007
Europe PMC | doi


Chiantia S, Ries J, Kahya N, Schwille P. (2006)

Combined AFM and two-focus SFCS study of raft-exhibiting model membranes

Chemphyschem 7(11):2409-2418. doi: 10.1002/cphc.200600464
Europe PMC | doi

Meacci G, Ries J, Fischer-Friedrich E, Kahya N, Schwille P, Kruse K. (2006)

Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy

Phys Biol 3(4):255-263. doi: 10.1088/1478-3975/3/4/003
Europe PMC | doi

Ries J, Schwille P. (2006)

Studying slow membrane dynamics with continuous wave scanning fluorescence correlation spectroscopy

Biophys. J. 91(5):1915-1924. doi: 10.1529/biophysj.106.082297
Europe PMC | doi

Chiantia S, Kahya N, Ries J, Schwille P. (2006)

Effects of ceramide on liquid-ordered domains investigated by simultaneous AFM and FCS

Biophys. J. 90(12):4500-4508. doi: 10.1529/biophysj.106.081026
Europe PMC | doi


Babichev SA, Ries J, Lvovsky AI. (2003)

Quantum scissors: Teleportation of single-mode optical states by means of a nonlocal single photon

Europhys Lett 64(1):1-7.
Europe PMC

Ries J, Brezger B, Lvovsky AI. (2003)

Experimental vacuum squeezing in rubidium vapor via self-rotation

Phys. Rev., A 68(2):025801.
Europe PMC