EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

ORCID iD: iD icon0000-0001-7098-0700


  • PhD in Biology, 2001, Ludwig Maximilians University, Munich.
    Microscopy specialist in the Advanced Light Microscopy Facility, EMBL Heidelberg.
    Head of the Advanced Light Microscopy Unit, Centre for Genomic Regulation, Barcelona, Spain.
    Team leader at EMBL from October 2020.


Total: 35 publication(s)


Zimmermann T. (2019)

Photobleaching and sensitized emission-based methods for the detection of Förster resonance energy transfer.

In: Rebollo E, Bosch M, Computer Optimized Microscopy, vol 2040. pp 235-274.Methods Mol Biol 2040:235-274. doi: 10.1007/978-1-4939-9686-5_12
Europe PMC | doi


Zimmermann T. (2017)

Superresolution Microscopy.

eLS doi: 10.1002/9780470015902.a0025317

Raote I, Ortega Bellido M, Pirozzi M, Zhang C, Melville D, Parashuraman S, Zimmermann T, Malhotra V. (2017)

TANGO1 assembles into rings around COPII coats at ER exit sites.

J Cell Biol 216(4):901-909. doi: 10.1083/jcb.201608080
Europe PMC | doi

Merino D, Mallabiabarrena A, Andilla J, Artigas D, Zimmermann T, Loza-Alvarez P. (2017)

STED imaging performance estimation by means of Fourier transform analysis.

Biomed Opt Express 8(5):2472-2482. doi: 10.1364/BOE.8.002472
Europe PMC | doi


Irazoki O, Aranda J, Zimmermann T, Campoy S, Barbé J. (2016)

Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming.

Front Microbiol 7 doi: 10.3389/fmicb.2016.01560
Europe PMC | doi

Jelier R, Kruger A, Swoger J, Zimmermann T, Lehner B. (2016)

Compensatory cell movements confer robustness to mechanical deformation during embryonic development.

Cell Syst 3(2):160-171. doi: 10.1016/j.cels.2016.07.005
Europe PMC | doi


Krüger AV, Jelier R, Dzyubachyk O, Zimmermann T, Meijering E, Lehner B. (2015)

Comprehensive single cell-resolution analysis of the role of chromatin regulators in early C. elegans embryogenesis.

Dev Biol 398(2):153-62. doi: 10.1016/j.ydbio.2014.10.014
Europe PMC | doi


Zimmermann T, Marrison J, Hogg K, O'Toole P. (2014)

Clearing up the signal: spectral imaging and linear unmixing in fluorescence microscopy.

Methods Mol Biol 1075:129-48. doi: 10.1007/978-1-60761-847-8_5
Europe PMC | doi

Agi E, Langen M, Altschuler SJ, Wu LF, Zimmermann T, Hiesinger PR. (2014)

The evolution and development of neural superposition.

J Neurogenet 28(3-4):216-32. doi: 10.3109/01677063.2014.922557
Europe PMC | doi

Carvalho A, Menendez DB, Senthivel VR, Zimmermann T, Diambra L, Isalan M. (2014)

Genetically encoded sender-receiver system in 3D mammalian cell culture.

ACS Synth Biol 3(5):264-72. doi: 10.1021/sb400053b
Europe PMC | doi

Kiel C, Ebhardt HA, Burnier J, Portugal C, Sabidó E, Zimmermann T, Aebersold R, Serrano L. (2014)

Quantification of ErbB network proteins in three cell types using complementary approaches identifies cell-general and cell-type-specific signaling proteins.

J Proteome Res 13(1):300-13. doi: 10.1021/pr400878x
Europe PMC | doi


Grünberg R, Burnier JV, Ferrar T, Beltran-Sastre V, Stricher F, van der Sloot AM, Garcia-Olivas R, Mallabiabarrena A, Sanjuan X, Zimmermann T, Serrano L. (2013)

Engineering of weak helper interactions for high-efficiency FRET probes.

Nat Methods 10(10):1021-7. doi: 10.1038/nmeth.2625
Europe PMC | doi


Kopp K, Buntru A, Pils S, Zimmermann T, Frank R, Zumbusch A, Hauck CR. (2012)

Grb14 is a negative regulator of CEACAM3-mediated phagocytosis of pathogenic bacteria.

J Biol Chem 287(46):39158-70. doi: 10.1074/jbc.M112.395228
Europe PMC | doi

Seitz A, Terjung S, Zimmermann T, Pepperkok R. (2012)

Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching-based fluorescence resonance energy transfer measurements.

J Biomed Opt 17(1):011010. doi: 10.1117/1.jbo.17.1.011010
Europe PMC | doi


von Blume J, Alleaume AM, Cantero-Recasens G, Curwin A, Carreras-Sureda A, Zimmermann T, van Galen J, Wakana Y, Valverde MA, Malhotra V. (2011)

ADF/Cofilin regulates secretory cargo sorting at the TGN via the Ca2+ ATPase SPCA1.

DEV CELL 20(5):652-662. doi: 10.1016/j.devcel.2011.03.014
Europe PMC | doi


Buntru A, Zimmermann T, Hauck CR. (2009)

Fluorescence resonance energy transfer (FRET)-based subcellular visualization of pathogen-induced host receptor signaling.

BMC Biol 7 doi: 10.1186/1741-7007-7-81
Europe PMC | doi

Bussmann LH, Schubert A, Vu Manh TP, De Andres L, Desbordes SC, Parra M, Zimmermann T, Rapino F, Rodriguez-Ubreva J, Ballestar E, Graf T. (2009)

A robust and highly efficient immune cell reprogramming system.

Cell Stem Cell 5(5):554-66. doi: 10.1016/j.stem.2009.10.004
Europe PMC | doi


Duran JM, Kinseth M, Bossard C, Rose DW, Polishchuk R, Wu CC, Yates J, Zimmerman T, Malhotra V. (2008)

The role of GRASP55 in Golgi fragmentation and entry of cells into mitosis.

Mol Biol Cell 19(6):2579-87. doi: 10.1091/mbc.e07-10-0998
Europe PMC | doi


Bilic J, Huang YL, Davidson G, Zimmermann T, Cruciat CM, Bienz M, Niehrs C. (2007)

Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylation.

Science 316(5831):1619-1622. doi: 10.1126/science.1137065
Europe PMC | doi

Ma B, Zimmermann T, Rohde M, Winkelbach S, He F, Lindenmaier W, Dittmar KE. (2007)

Use of Autostitch for automatic stitching of microscope images.

Micron 38(5):492-499. doi: 10.1016/j.micron.2006.07.027
Europe PMC | doi


Brunet S, Zimmermann T, Reynaud EG, Vernos I, Karsenti E, Pepperkok R. (2006)

Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion.

Traffic 7(9):1283-1289. doi: 10.1111/j.1600-0854.2006.00463.x
Europe PMC | doi

Forster R, Weiss M, Zimmermann T, Reynaud EG, Verissimo F, Stephens DJ, Pepperkok R. (2006)

Secretory cargo regulates the turnover of COPII subunits at single ER exit sites.

Curr. Biol. 16(2):173-179. doi: 10.1016/j.cub.2005.11.076
Europe PMC | doi


Moita LF, Wang-Sattler R, Michel K, Zimmermann T, Blandin S, Levashina EA, Kafatos FC. (2005)

In vivo identification of novel regulators and conserved pathways of phagocytosis in A. gambiae.

Immunity 23(1):65-73. doi: 10.1016/j.immuni.2005.05.006
Europe PMC | doi

Gräf R, Rietdorf J, Zimmermann T. (2005)

Live cell spinning disk microscopy.

Adv. Biochem. Eng. Biotechnol. 95:57-75. doi: 10.1007/b102210
Europe PMC | doi

Pepperkok R, Simpson JC, Rietdorf J, Cetin C, Liebel U, Terjung S, Zimmermann T. (2005)

Imaging platforms for measurement of membrane trafficking.

Meth. Enzymol. 404:8-18. doi: 10.1016/s0076-6879(05)04002-4
Europe PMC | doi

Zimmermann T. (2005)

Spectral imaging and linear unmixing in light microscopy.

Adv. Biochem. Eng. Biotechnol. 95:245-265. doi: 10.1007/b102216
Europe PMC | doi


Brunet S, Sardon T, Zimmerman T, Wittmann T, Pepperkok R, Karsenti E, Vernos I. (2004)

Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts.

Mol. Biol. Cell 15(12):5318-5328. doi: 10.1091/mbc.e04-05-0385
Europe PMC | doi

Vlachou D, Zimmermann T, Cantera R, Janse CJ, Waters AP, Kafatos FC. (2004)

Real-time, in vivo analysis of malaria ookinete locomotion and mosquito midgut invasion.

Cell. Microbiol. 6(7):671-685. doi: 10.1111/j.1462-5822.2004.00394.x
Europe PMC | doi


Zimmermann T, Rietdorf J, Pepperkok R. (2003)

Spectral imaging and its applications in live cell microscopy.

FEBS Lett. 546(1):87-92. doi: 10.1016/s0014-5793(03)00521-0
Europe PMC | doi


Zimmermann T, Rietdorf J, Girod A, Georget V, Pepperkok R. (2002)

Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair.

FEBS Lett. 531(2):245-249. doi: 10.1016/s0014-5793(02)03508-1
Europe PMC | doi


Rietdorf J, Ploubidou A, Reckmann I, Holmström A, Frischknecht F, Zettl M, Zimmermann T, Way M. (2001)

Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus.

Nat. Cell Biol. 3(11):992-1000. doi: 10.1038/ncb1101-992
Europe PMC | doi


Zimmermann T, Siegert F. (1998)

4D confocal microscopy of Dictyostelium discoideum morphogenesis and its presentation on the Internet.

Dev Genes Evol 208(7):411-20. doi: 10.1007/s004270050198
Europe PMC | doi

Zimmermann T, Siegert F. (1998)

Simultaneous detection of two GFP spectral mutants during in vivo confocal microscopy of migrating Dictyostelium cells.

Biotechniques 24(3):458-61. doi: 10.2144/98243st06
Europe PMC | doi


Melzer RR, Zimmermann T, Smola U. (1997)

Modification of dispersal patterns of branched photoreceptor axons and the evolution of neural superposition.

Cell. Mol. Life Sci. 53(3) doi: 10.1007/PL00000598


Petykó Z, Zimmermann T, Smola U, Melzer RR. (1996)

Central projections of Tömösváry's organ in Lithobius forficatus (Chilopoda, Lithobiidae).

Cell Tissue Res 283(2):331-4. doi: 10.1007/s004410050543
Europe PMC | doi