EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
Biography
PhD 2006, Institute of Microbiology and Plant Physiology, Freie Universität Berlin, Germany.
Postgraduate research, University of California, San Francisco.
Group leader at EMBL since 2011.
Joint appointment with the Structural and Computational Biology Unit.
Humboldt Sofja Kovalevskaja Award winner 2012.
Senior scientist since 2020.
ERC Investigator.
Publications
Total: 59 publication(s)
2020
Mateus A, Hevler J, Bobonis J, Kurzawa N, Shah M, Mitosch K, Goemans CV, Helm D, Stein F, Typas A, Savitski MM. (2020)
The functional proteome landscape of Escherichia coli.
Nature 588(7838):473-478. doi: 10.1038/s41586-020-3002-5
Europe PMC | doi
Typas A, Li GW. (2020)
Editorial overview: microbial systems biology.
Curr Opin Microbiol 57 doi: 10.1016/j.mib.2020.11.004
Europe PMC | doi
Selkrig J, Stanifer M, Mateus A, Mitosch K, Barrio-Hernandez I, Rettel M, Kim H, Voogdt C, Walch P, Kee C, Kurzawa N, Stein F, Potel C, Jarzab A, Kuster B, Bartenschlager R, Boulant S, Beltrao P, Typas A, Savitski MM. (2020)
SARS-CoV-2 infection remodels the host protein thermal stability landscape.
Research Square doi: 10.21203/rs.3.rs-105193/v1
doi
Bobonis J, Mateus A, Pfalz B, Garcia-Santamarina S, Galardini M, Kobayashi C, Stein F, Savitski MM, Elfenbein JR, Andrews-Poymenis H, Typas A. (2020)
Bacterial retrons encode tripartite toxin/antitoxin systems.
bioRxivorg doi: 10.1101/2020.06.22.160168
doi
Selkrig J, Li N, Hausmann A, Mangan MSJ, Zietek M, Mateus A, Bobonis J, Sueki A, Imamura H, El Debs B, Sigismondo G, Florea BI, Overkleeft HS, Kopitar-Jerala N, Turk B, Beltrao P, Savitski MM, Latz E, Hardt WD, Krijgsveld J, Typas A. (2020)
Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection.
Nat Microbiol 5(9):1119-1133. doi: 10.1038/s41564-020-0736-7
Europe PMC | doi
Imai Y, Meyer KJ, Iinishi A, Favre-Godal Q, Green R, Manuse S, Caboni M, Mori M, Niles S, Ghiglieri M, Honrao C, Ma X, Guo JJ, Makriyannis A, Linares-Otoya L, Böhringer N, Wuisan ZG, Kaur H, Wu R, Mateus A, Typas A, Savitski MM, Espinoza JL, O'Rourke A, Nelson KE, Hiller S, Noinaj N, Schäberle TF, D'Onofrio A, Lewis K. (2020)
Author correction: a new antibiotic selectively kills Gram-negative pathogens.
Nature 580(7802) doi: 10.1038/s41586-020-2063-9
Europe PMC | doi
Bobonis J, Mitosch K, Mateus A, Kritikos G, Elfenbein JR, Savitski MM, Andrews-Polymenis H, Typas A. (2020)
Phage proteins block and trigger retron toxin/antitoxin systems.
bioRxivorg doi: 10.1101/2020.06.22.160242
doi
Martin JK, Sheehan JP, Bratton BP, Moore GM, Mateus A, Li SH, Kim H, Rabinowitz JD, Typas A, Savitski MM, Wilson MZ, Gitai Z. (2020)
A dual-mechanism antibiotic kills gram-negative bacteria and avoids drug resistance.
Cell 181(7) doi: 10.1016/j.cell.2020.05.005
Europe PMC | doi
Vonesch SC, Bredikhin D, Dobrev N, Villacorta L, Kleinendorst R, Cacace E, Flock J, Frank M, Jung F, Kornienko J, Mitosch K, Osuna-Lopez M, Zimmermann J, Goettig S, Hamprecht A, Kraeusslich H, Knop M, Typas A, Steinmetz LM, Benes V, Remans K, Krebs AR. (2020)
McQ – an open-source multiplexed SARS-CoV-2 quantification platform.
medRxiv preprint doi: 10.1101/2020.12.02.20242628
doi
Mateus A, Kurzawa N, Becher I, Sridharan S, Helm D, Stein F, Typas A, Savitski MM. (2020)
Thermal proteome profiling for interrogating protein interactions.
Mol. Syst. Biol. 16(3) doi: 10.15252/msb.20199232
Europe PMC | doi
Sueki A, Stein F, Savitski MM, Selkrig J, Typas A. (2020)
Systematic localization of Escherichia coli membrane proteins.
mSystems 5(2) doi: 10.1128/mSystems.00808-19
Europe PMC | doi
Domenech A, Brochado AR, Sender V, Hentrich K, Henriques-Normark B, Typas A, Veening JW. (2020)
Proton motive force disruptors block bacterial competence and horizontal gene transfer.
Cell Host Microbe 27(4) doi: 10.1016/j.chom.2020.02.002
Europe PMC | doi
Banzhaf M, Yau HC, Verheul J, Lodge A, Kritikos G, Mateus A, Cordier B, Hov AK, Stein F, Wartel M, Pazos M, Solovyova AS, Breukink E, van Teeffelen S, Savitski MM, den Blaauwen T, Typas A, Vollmer W. (2020)
Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi-enzyme complexes in Escherichia coli.
EMBO J. 39(5) doi: 10.15252/embj.2019102246
Europe PMC | doi
2019
Galardini M, Busby BP, Vieitez C, Dunham AS, Typas A, Beltrao P. (2019)
The impact of the genetic background on gene deletion phenotypes in Saccharomyces cerevisiae.
Mol. Syst. Biol. 15(12) doi: 10.15252/msb.20198831
Europe PMC | doi
Foerster S, Gustafsson TN, Rita Brochado A, Desilvestro V, Typas A, Unemo M. (2019)
The first wide-scale drug repurposing screen using the Prestwick Chemical Library (1200 bioactive molecules) against Neisseria gonorrhoeae identifies high in vitro activity of auranofin and many additional drugs.
APMIS 128(3):242-250. doi: 10.1111/apm.13014
Europe PMC | doi
Wotzka SY, Kreuzer M, Maier L, Arnoldini M, Nguyen BD, Brachmann AO, Berthold DL, Zund M, Hausmann A, Bakkeren E, Hoces D, Gul E, Beutler M, Dolowschiak T, Zimmermann M, Fuhrer T, Moor K, Sauer U, Typas A, Piel J, Diard M, Macpherson AJ, Stecher B, Sunagawa S, Slack E, Hardt WD. (2019)
Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice.
Nat Microbiol 4(12):2164-2174. doi: 10.1038/s41564-019-0568-5
Europe PMC | doi
Imai Y, Meyer KJ, Iinishi A, Favre-Godal Q, Green R, Manuse S, Caboni M, Mori M, Niles S, Ghiglieri M, Honrao C, Ma X, Guo J, Makriyannis A, Linares-Otoya L, Wuisan ZG, Böhringer N, Wu R, Kaur H, Typas A, Mateus A, Espinoza JL, Savitski MM, Nelson KE, O'Rourke A, Noinaj N, Hiller S, D'Onofrio A, Schäberle TF, Lewis K. (2019)
A new antibiotic selectively kills Gram-negative pathogens.
Nature 576(7787):459-464. doi: 10.1038/s41586-019-1791-1
Europe PMC | doi
2018
Wagih O, Galardini M, Busby BP, Memon D, Typas A, Beltrao P. (2018)
A resource of variant effect predictions of single nucleotide variants in model organisms.
Mol. Syst. Biol. 14(12):e8430. doi: 10.15252/msb.20188430
Europe PMC | doi
Palleja A, Mikkelsen KH, Forslund SK, Kashani A, Allin KH, Nielsen T, Hansen TH, Liang S, Feng Q, Zhang C, Pyl PT, Coelho LP, Yang H, Wang J, Typas A, Nielsen HB, Nielsen MF, Wang J, Bork P, Hansen T, Vilsbøll T, Arumugam M, Knop FK, Pedersen O. (2018)
Recovery of gut microbiota of healthy adults following antibiotic exposure.
Nat Microbiol 3(11) doi: 10.1038/s41564-018-0257-9
Europe PMC | doi
Mateus A, Bobonis J, Kurzawa N, Stein F, Helm D, Hevler J, Typas A, Savitski MM. (2018)
Thermal proteome profiling in bacteria: probing protein state in vivo.
Mol. Syst. Biol. 14(7) doi: 10.15252/msb.20188242
Europe PMC | doi
Brochado AR, Telzerow A, Bobonis J, Banzhaf M, Mateus A, Selkrig J, Huth E, Bassler S, Zamarreño Beas J, Zietek M, Ng N, Foerster S, Ezraty B, Py B, Barras F, Bork P, Savitski MM, Göttig S, Typas A. (2018)
Species-specific activity of antibacterial drug combinations.
Nature 559(7713):259-263. doi: 10.1038/s41586-018-0278-9
Europe PMC | doi
Becher I, Andrés-Pons A, Romanov N, Stein F, Schramm M, Baudin F, Helm D, Kurzawa N, Mateus A, Mackmull MT, Typas A, Müller CW, Bork P, Beck M, Savitski MM. (2018)
Pervasive protein thermal stability variation during the cell cycle.
Cell 173(6) doi: 10.1016/j.cell.2018.03.053
Europe PMC | doi
Tramontano M, Andrejev S, Pruteanu M, Klünemann M, Kuhn M, Galardini M, Jouhten P, Zelezniak A, Zeller G, Bork P, Typas A, Patil KR. (2018)
Nutritional preferences of human gut bacteria reveal their metabolic idiosyncrasies.
Nat Microbiol 3(4) doi: 10.1038/s41564-018-0123-9
Europe PMC | doi
Maier L, Pruteanu M, Kuhn M, Zeller G, Telzerow A, Anderson EE, Brochado AR, Fernandez KC, Dose H, Mori H, Patil KR, Bork P, Typas A. (2018)
Extensive impact of non-antibiotic drugs on human gut bacteria.
Nature 555(7698) doi: 10.1038/nature25979
Europe PMC | doi
Rancati G, Moffat J, Typas A, Pavelka N. (2018)
Emerging and evolving concepts in gene essentiality.
Nat Rev Genet 19(1) doi: 10.1038/nrg.2017.74
Europe PMC | doi
2017
Galardini M, Koumoutsi A, Herrera-Dominguez L, Cordero Varela JA, Telzerow A, Wagih O, Wartel M, Clermont O, Denamur E, Typas A, Beltrao P. (2017)
Phenotype inference in an Escherichia coli strain panel.
Elife 6 doi: 10.7554/eLife.31035
Europe PMC | doi
Wilck N, Matus MG, Kearney SM, Olesen SW, Forslund K, Bartolomaeus H, Haase S, Mähler A, Balogh A, Markó L, Vvedenskaya O, Kleiner FH, Tsvetkov D, Klug L, Costea PI, Sunagawa S, Maier L, Rakova N, Schatz V, Neubert P, Frätzer C, Krannich A, Gollasch M, Grohme DA, Côrte-Real BF, Gerlach RG, Basic M, Typas A, Wu C, Titze JM, Jantsch J, Boschmann M, Dechend R, Kleinewietfeld M, Kempa S, Bork P, Linker RA, Alm EJ, Müller DN. (2017)
Salt-responsive gut commensal modulates TH17 axis and disease.
Nature 551(7682):585-589. doi: 10.1038/nature24628
Europe PMC | doi
Ponomarova O, Gabrielli N, Sévin DC, Mülleder M, Zirngibl K, Bulyha K, Andrejev S, Kafkia E, Typas A, Sauer U, Ralser M, Patil KR. (2017)
Yeast Creates a Niche for Symbiotic Lactic Acid Bacteria through Nitrogen Overflow.
Cell Syst S2405-4712(17):30390-30393. doi: 10.1016/j.cels.2017.09.002
Europe PMC | doi
Maier L, Typas A. (2017)
Systematically investigating the impact of medication on the gut microbiome.
Curr. Opin. Microbiol. 39:128-135. doi: 10.1016/j.mib.2017.11.001
Europe PMC | doi
Nikolay F, Pesavento M, Kritikos G, Typas N. (2017)
Learning directed acyclic graphs from large-scale genomics data.
EURASIP J Bioinform Syst Biol 2017(1) doi: 10.1186/s13637-017-0063-3
Europe PMC | doi
Cacace E, Kritikos G, Typas A. (2017)
Chemical genetics in drug discovery.
Curr Opin Syst Biol 4:35-42. doi: 10.1016/j.coisb.2017.05.020
doi
Scott TA, Quintaneiro LM, Norvaisas P, Lui PP, Wilson MP, Leung KY, Herrera-Dominguez L, Sudiwala S, Pessia A, Clayton PT, Bryson K, Velagapudi V, Mills PB, Typas A, Greene NDE, Cabreiro F. (2017)
Host-Microbe Co-metabolism Dictates Cancer Drug Efficacy in C. elegans.
Cell 169(3):442-456.e18. doi: 10.1016/j.cell.2017.03.040
Europe PMC | doi
Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA.. (2017)
Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis.
Cell Syst 4(16) doi: 10.1016/j.cels.2016.12.013
Europe PMC | doi
Kritikos G, Banzhaf M, Herrera-Dominguez L, Koumoutsi A, Wartel M, Zietek M, Typas A.. (2017)
A tool named Iris for versatile high-throughput phenotyping in microorganisms.
Nat Microbiol 2(2) doi: 10.1038/nmicrobiol.2017.14
Europe PMC | doi
2016
Ochoa D, Jonikas M, Lawrence RT, El Debs B, Selkrig J, Typas A, Villén J, Santos SD, Beltrao P. (2016)
An atlas of human kinase regulation.
Mol. Syst. Biol. 12(12):888. doi: 10.15252/msb.20167295
Europe PMC | doi
Shiver AL, Osadnik H, Kritikos G, Li B, Krogan N, Typas A, Gross CA. (2016)
A Chemical-Genomic Screen of Neglected Antibiotics Reveals Illicit Transport of Kasugamycin and Blasticidin S.
PLoS Genet. 12(6):e1006124. doi: 10.1371/journal.pgen.1006124
Europe PMC | doi
Chen WH, van Noort V, Lluch-Senar M, Hennrich ML, H Wodke JA, Yus E, Alibés A, Roma G, Mende DR, Pesavento C, Typas A, Gavin AC, Serrano L, Bork P. (2016)
Integration of multi-omics data of a genome-reduced bacterium: Prevalence of post-transcriptional regulation and its correlation with protein abundances.
Nucleic Acids Res. 44(3) doi: 10.1093/nar/gkw004
Europe PMC | doi
2015
Brown ED, Typas A. (2015)
Editorial overview: microbial systems biology.
Curr Opin Microbiol 27 doi: 10.1016/j.mib.2015.09.007
Europe PMC | doi
Typas A, Sourjik V. (2015)
Bacterial protein networks: properties and functions.
Nat. Rev. Microbiol. 13(9):559-572. doi: 10.1038/nrmicro3508
Europe PMC | doi
Gray AN, Egan AJ, Van't Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar MA, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W. (2015)
Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division.
Elife 4 doi: 10.7554/elife.07118
Europe PMC | doi
2014
Cho SH, Szewczyk J, Pesavento C, Zietek M, Banzhaf M, Roszczenko P, Asmar A, Laloux G, Hov AK, Leverrier P, Van der Henst C, Vertommen D, Typas A, Collet JF. (2014)
Detecting Envelope Stress by Monitoring β-Barrel Assembly.
Cell 159(7):1652-1664. doi: 10.1016/j.cell.2014.11.045
Europe PMC | doi
Egan AJ, Jean NL, Koumoutsi A, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A, Vollmer W, Simorre JP. (2014)
Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B.
Proc. Natl. Acad. Sci. U.S.A. 111(22):8197-8202. doi: 10.1073/pnas.1400376111
Europe PMC | doi
Banzhaf M, Typas A. (2014)
Dynamic protein complexes for cell growth
Proc. Natl. Acad. Sci. U.S.A. 111(12):4355-4356. doi: 10.1073/pnas.1402016111
Europe PMC | doi
Paradis-Bleau C, Kritikos G, Orlova K, Typas A, Bernhardt TG. (2014)
A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism
PLoS Genet. 10(1):e1004056. doi: 10.1371/journal.pgen.1004056
Europe PMC | doi
2013
Forslund K, Sunagawa S, Kultima JR, Mende DR, Arumugam M, Typas A, Bork P. (2013)
Country-specific antibiotic use practices impact the human gut resistome
Genome Res. 23(7):1163-1169. doi: 10.1101/gr.155465.113
Europe PMC | doi
Ezraty B, Vergnes A, Banzhaf M, Duverger Y, Huguenot A, Brochado AR, Su SY, Espinosa L, Loiseau L, Py B, Typas A, Barras F. (2013)
Fe-S cluster biosynthesis controls uptake of aminoglycosides in a ROS-less death pathway
Science 340(6140):1583-1587. doi: 10.1126/science.1238328
Europe PMC | doi
Brochado AR, Typas A. (2013)
High-throughput approaches to understanding gene function and mapping network architecture in bacteria
Curr. Opin. Microbiol. 16(2):199-206. doi: 10.1016/j.mib.2013.01.008
Europe PMC | doi
2012
Typas A, Banzhaf M, Gross CA, Vollmer W. (2012)
From the regulation of peptidoglycan synthesis to bacterial growth and morphology
Nat. Rev. Microbiol. 10(2):123-136. doi: 10.1038/nrmicro2677
Europe PMC | doi
2011
Oh E, Becker AH, Sandikci A, Huber D, Chaba R, Gloge F, Nichols RJ, Typas A, Gross CA, Kramer G, Weissman JS, Bukau B. (2011)
Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo
Cell 147(6):1295-1308. doi: 10.1016/j.cell.2011.10.044
Europe PMC | doi
Singh SS, Typas A, Hengge R, Grainger DC. (2011)
Escherichia coli σ⁷⁰ senses sequence and conformation of the promoter spacer region
Nucleic Acids Res. 39(12):5109-5118. doi: 10.1093/nar/gkr080
Europe PMC | doi
Nichols RJ, Sen S, Choo YJ, Beltrao P, Zietek M, Chaba R, Lee S, Kazmierczak KM, Lee KJ, Wong A, Shales M, Lovett S, Winkler ME, Krogan NJ, Typas A, Gross CA. (2011)
Phenotypic landscape of a bacterial cell
Cell 144(1):143-156. doi: 10.1016/j.cell.2010.11.052
Europe PMC | doi
2010
Typas A, Banzhaf M, van den Berg van Saparoea B, Verheul J, Biboy J, Nichols RJ, Zietek M, Beilharz K, Kannenberg K, von Rechenberg M, Breukink E, den Blaauwen T, Gross CA, Vollmer W. (2010)
Regulation of peptidoglycan synthesis by outer-membrane proteins
Cell 143(7):1097-1109. doi: 10.1016/j.cell.2010.11.038
Europe PMC | doi
2008
Typas A, Nichols RJ, Siegele DA, Shales M, Collins SR, Lim B, Braberg H, Yamamoto N, Takeuchi R, Wanner BL, Mori H, Weissman JS, Krogan NJ, Gross CA. (2008)
High-throughput, quantitative analyses of genetic interactions in E. coli
Nat. Methods 5(9):781-787. doi: 10.1038/nmeth.1240
Europe PMC | doi
2007
Typas A, Barembruch C, Possling A, Hengge R. (2007)
Stationary phase reorganisation of the Escherichia coli transcription machinery by Crl protein, a fine-tuner of sigmas activity and levels
EMBO J. 26(6):1569-1578. doi: 10.1038/sj.emboj.7601629
Europe PMC | doi
Typas A, Becker G, Hengge R. (2007)
The molecular basis of selective promoter activation by the sigmaS subunit of RNA polymerase
Mol. Microbiol. 63(5):1296-1306. doi: 10.1111/j.1365-2958.2007.05601.x
Europe PMC | doi
Typas A, Stella S, Johnson RC, Hengge R. (2007)
The -35 sequence location and the Fis-sigma factor interface determine sigmas selectivity of the proP (P2) promoter in Escherichia coli
Mol. Microbiol. 63(3):780-796. doi: 10.1111/j.1365-2958.2006.05560.x
Europe PMC | doi
Klauck E, Typas A, Hengge R. (2007)
The sigmaS subunit of RNA polymerase as a signal integrator and network master regulator in the general stress response in Escherichia coli
Sci Prog 90(Pt 2-3):103-127.
Europe PMC
2006
Typas A, Hengge R. (2006)
Role of the spacer between the -35 and -10 regions in sigmas promoter selectivity in Escherichia coli
Mol. Microbiol. 59(3):1037-1051. doi: 10.1111/j.1365-2958.2005.04998.x
Europe PMC | doi
2005
Typas A, Hengge R. (2005)
Differential ability of sigma(s) and sigma70 of Escherichia coli to utilize promoters containing half or full UP-element sites
Mol. Microbiol. 55(1):250-260. doi: 10.1111/j.1365-2958.2004.04382.x
Europe PMC | doi