EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
Phone: +49 6221 387-8626
Biography
PhD 2005, Eötvös Loránd University, Budapest, Hungary.
Postdoctoral research at the National Institutes of Health, Bethesda, USA.
Group leader at EMBL since 2009.
Publications
Total: 41 publication(s)
2020
Köhn M, Salvi F, Hoermann B, Del Pino Garcia J, Fontanillo M, Derua R, Beullens M, Bollen M, Barabas O. (2020)
Towards dissecting the mechanism of protein phosphatase-1 inhibition by its C-terminal phosphorylation.
Chembiochem 22(5) doi: 10.1002/cbic.202000669
Europe PMC | doi
Kesselring L, Miskey C, Zuliani C, Querques I, Kapitonov V, Laukó A, Fehér A, Palazzo A, Diem T, Lustig J, Sebe A, Wang Y, Dinnyés A, Izsvák Z, Barabas O, Ivics Z. (2020)
A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.
Nucleic Acids Res. 48(1):316-331. doi: 10.1093/nar/gkz1119
Europe PMC | doi
2019
Barabas O. (2019)
Snapshots of a genetic cut-and-paste
Nature 575(7783):447-448. doi: 10.1038/d41586-019-03428-0
Europe PMC | doi
Querques I, Mades A, Zuliani C, Miskey C, Alb M, Grueso E, Machwirth M, Rausch T, Einsele H, Ivics Z, Hudecek M, Barabas O. (2019)
A highly soluble Sleeping Beauty transposase improves control of gene insertion.
Nat. Biotechnol. 37(12):1502-1512. doi: 10.1038/s41587-019-0291-z
Europe PMC | doi
Arinkin V, Smyshlyaev G, Barabas O. (2019)
Jump ahead with a twist: DNA acrobatics drive transposition forward.
Curr. Opin. Struct. Biol. 59:168-177. doi: 10.1016/j.sbi.2019.08.006
Europe PMC | doi
Smyshlyaev G, Barabas O, Bateman A. (2019)
Sequence analysis allows functional annotation of tyrosine recombinases in prokaryotic genomes.
bioRxivorg doi: 10.1101/542381
doi
Weng C, Kosalka J, Berkyurek AC, Stempor P, Feng X, Mao H, Zeng C, Li WJ, Yan YH, Dong MQ, Morero NR, Zuliani C, Barabas O, Ahringer J, Guang S, Miska EA. (2019)
The USTC co-opts an ancient machinery to drive piRNA transcription in C. elegans.
Genes Dev. 33(1-2):90-102. doi: 10.1101/gad.319293.118
Europe PMC | doi
2018
Salvi F, Trebacz M, Kokot T, Hörmann B, Rios P, Barabas O, Köhn M. (2018)
Effects of stably incorporated iron on Protein Phosphatase-1 structure and activity.
FEBS Lett. 592(24) doi: 10.1002/1873-3468.13284
Europe PMC | doi
Morero NR, Zuliani C, Kumar B, Bebel A, Okamoto S, Guynet C, Hickman AB, Chandler M, Dyda F, Barabas O. (2018)
Targeting IS608 transposon integration to highly specific sequences by structure-based transposon engineering
Nucleic Acids Res. 46(8):4152-4163. doi: 10.1093/nar/gky235
Europe PMC | doi
Rubio-Cosials A, Schulz EC, Lambertsen L, Smyshlyaev G, Rojas-Cordova C, Forslund K, Karaca E, Bebel A, Bork P, Barabas O. (2018)
Transposase-DNA Complex Structures Reveal Mechanisms for Conjugative Transposition of Antibiotic Resistance.
Cell 173(1):208-220.e20. doi: 10.1016/j.cell.2018.02.032
Europe PMC | doi
Lambertsen L, Rubio-Cosials A, Patil KR, Barabas O. (2018)
Conjugative transposition of the vancomycin resistance carrying Tn1549: enzymatic requirements and target site preferences.
Mol. Microbiol. 107(5) doi: 10.1111/mmi.13905
Europe PMC | doi
2017
Schulz EC, Seiler M, Zuliani C, Voigt F, Rybin V, Pogenberg V, Mücke N, Wilmanns M, Gibson TJ, Barabas O.. (2017)
Intermolecular base stacking mediates RNA-RNA interaction in a crystal structure of the RNA chaperone Hfq
Sci Rep 7(1):9903. doi: 10.1038/s41598-017-10085-8
Europe PMC | doi
2016
Bebel A, Karaca E, Kumar B, Stark WM, Barabas O. (2016)
Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending.
Elife 5 doi: 10.7554/elife.19706
Europe PMC | doi
Abrusán G , Yant SR , Szilágyi A , Marsh JA , Mátés L , Izsvák Z , Barabás O , Ivics Z. (2016)
Structural Determinants of Sleeping Beauty Transposase Activity.
Mol. Ther. 24(8):1369-1377. doi: 10.1038/mt.2016.110
Europe PMC | doi
Voigt F, Wiedemann L, Zuliani C, Querques I, Sebe A, Mátés L, Izsvák Z, Ivics Z, Barabas O. (2016)
Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.
Nat Commun 7 doi: 10.1038/ncomms11126
Europe PMC | doi
2014
Bock T, Chen WH, Ori A, Malik N, Silva-Martin N, Huerta-Cepas J, Powell ST, Kastritis PL, Smyshlyaev G, Vonkova I, Kirkpatrick J, Doerks T, Nesme L, Baßler J, Kos M, Hurt E, Carlomagno T, Gavin AC, Barabas O, Müller CW, van Noort V, Beck M, Bork P. (2014)
An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum.
Nucleic Acids Res. 42(22):13525-13533. doi: 10.1093/nar/gku1147
Europe PMC | doi
Schulz EC, Barabas O. (2014)
Structure of an Escherichia coli Hfq:RNA complex at 0.97 A resolution.
Acta Crystallogr F Struct Biol Commun 70(Pt 11):1492-1497. doi: 10.1107/s2053230x14020044
Europe PMC | doi
2013
Barabás O, Németh V, Bodor A, Perczel A, Rosta E, Kele Z, Zagyva I, Szabadka Z, Grolmusz VI, Wilmanns M, Vértessy BG. (2013)
Catalytic mechanism of α-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling
Nucleic Acids Res. 41(22):10542-10555. doi: 10.1093/nar/gkt756
Europe PMC | doi
Smyshlyaev G, Voigt F, Blinov A, Barabas O, Novikova O. (2013)
Acquisition of an Archaea-like ribonuclease H domain by plant L1 retrotransposons supports modular evolution
Proc. Natl. Acad. Sci. U.S.A. 110(50):20140-20145. doi: 10.1073/pnas.1310958110
Europe PMC | doi
2012
Voigt F, Reuter M, Kasaruho A, Schulz EC, Pillai RS, Barabas O. (2012)
Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc
RNA 18(12):2128-2134. doi: 10.1261/rna.034967.112
Europe PMC | doi
Barabas O. (2012)
Mechanism of Single-Stranded DNA Transposition: A Structural Perspective.
In: Wiley Online Library, eLS. John Wiley & Sons Ltd:, Chichester.doi: 10.1002/9780470015902.a0023178
doi
2010
Hickman AB, James JA, Barabas O, Pasternak C, Ton-Hoang B, Chandler M, Sommer S, Dyda F. (2010)
DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans
EMBO J. 29(22):3840-3852. doi: 10.1038/emboj.2010.241
Europe PMC | doi
2009
Guynet C, Achard A, Hoang BT, Barabas O, Hickman AB, Dyda F, Chandler M. (2009)
Resetting the site: redirecting integration of an insertion sequence in a predictable way
Mol. Cell 34(5):612-619. doi: 10.1016/j.molcel.2009.05.017
Europe PMC | doi
Takács E, Barabás O, Petoukhov MV, Svergun DI, Vértessy BG. (2009)
Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomers
FEBS Lett. 583(5):865-871. doi: 10.1016/j.febslet.2009.02.011
Europe PMC | doi
Kersulyte D, Lee W, Subramaniam D, Anant S, Herrera P, Cabrera L, Balqui J, Barabas O, Kalia A, Gilman RH, Berg DE. (2009)
Helicobacter Pylori's plasticity zones are novel transposable elements
PLoS ONE 4(9):e6859. doi: 10.1371/journal.pone.0006859
Europe PMC | doi
2008
Varga B, Barabás O, Takács E, Nagy N, Nagy P, Vértessy BG. (2008)
Active site of mycobacterial dUTPase: structural characteristics and a built-in sensor
Biochem. Biophys. Res. Commun. 373(1):8-13. doi: 10.1016/j.bbrc.2008.05.130
Europe PMC | doi
Kovári J, Barabás O, Varga B, Békési A, Tölgyesi F, Fidy J, Nagy J, Vértessy BG. (2008)
Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site
Proteins 71(1):308-319. doi: 10.1002/prot.21757
Europe PMC | doi
Kardos J, Harmat V, Palló A, Barabás O, Szilágyi K, Gráf L, Náray-Szabó G, Goto Y, Závodszky P, Gál P. (2008)
Revisiting the mechanism of the autoactivation of the complement protease C1r in the C1 complex: structure of the active catalytic region of C1r
Mol. Immunol. 45(6):1752-1760. doi: 10.1016/j.molimm.2007.09.031
Europe PMC | doi
Guynet C, Hickman AB, Barabas O, Dyda F, Chandler M, Ton-Hoang B. (2008)
In vitro reconstitution of a single-stranded transposition mechanism of IS608
Mol. Cell 29(3):302-312. doi: 10.1016/j.molcel.2007.12.008
Europe PMC | doi
Barabas O, Ronning DR, Guynet C, Hickman AB, Ton-Hoang B, Chandler M, Dyda F. (2008)
Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection
Cell 132(2):208-220. doi: 10.1016/j.cell.2007.12.029
Europe PMC | doi
2007
Varga B, Barabás O, Kovári J, Tóth J, Hunyadi-Gulyás E, Klement E, Medzihradszky KF, Tölgyesi F, Fidy J, Vértessy BG. (2007)
Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase
FEBS Lett. 581(24):4783-4788. doi: 10.1016/j.febslet.2007.09.005
Europe PMC | doi
Németh-Pongrácz V, Barabás O, Fuxreiter M, Simon I, Pichová I, Rumlová M, Zábranská H, Svergun D, Petoukhov M, Harmat V, Klement E, Hunyadi-Gulyás E, Medzihradszky KF, Kónya E, Vértessy BG. (2007)
Flexible segments modulate co-folding of dUTPase and nucleocapsid proteins
Nucleic Acids Res. 35(2):495-505. doi: 10.1093/nar/gkl1074
Europe PMC | doi
2006
Barabás O, Németh V, Vértessy BG. (2006)
Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62(Pt 4):399-401. doi: 10.1107/s1744309106008931
Europe PMC | doi
2004
Barabás O, Pongrácz V, Kovári J, Wilmanns M, Vértessy BG. (2004)
Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase
J. Biol. Chem. 279(41):42907-42915. doi: 10.1074/jbc.m406135200
Europe PMC | doi
Kovári J, Barabás O, Takács E, Békési A, Dubrovay Z, Pongrácz V, Zagyva I, Imre T, Szabó P, Vértessy BG. (2004)
Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: kinetic characterization, folding, and crystallographic studies of the homotrimeric Drosophila enzyme
J. Biol. Chem. 279(17):17932-17944. doi: 10.1074/jbc.m313643200
Europe PMC | doi
2003
Barabás O, Rumlová M, Erdei A, Pongrácz V, Pichová I, Vértessy BG. (2003)
dUTPase and nucleocapsid polypeptides of the Mason-Pfizer monkey virus form a fusion protein in the virion with homotrimeric organization and low catalytic efficiency
J. Biol. Chem. 278(40):38803-38812. doi: 10.1074/jbc.m306967200
Europe PMC | doi
2002
Beke G, Gergely A, Szász G, Szentesi A, Nyitray J, Barabás O, Harmath V, Mátyus P. (2002)
Synthesis and stereochemistry of dispiro substituted pyridazines: application of ellipticity-absorbance ratio spectra for proving enantiomeric relationship by HPLC-CD/UV detection
Chirality 14(5):365-371. doi: 10.1002/chir.10096
Europe PMC | doi
2001
Abran A, Csampai A, Kotschy A, Barabas O, Sohar P. (2001)
Study on ferrocenes, part 9 1 substrate selective transformations of some ferrocenylhydrazones
J Mol Struct 569(1-3):185-194.
Europe PMC
Elias O, Karolyhazy L, Stajer G, Fulop F, Czako K, Harmath V, Barabas O, Keseru K, Matyus P. (2001)
Theoretical and experimental studies on ring closure reactions of 4(5)-chloro-5(4)-hydroxyalkylamino-6-nitro-3 (2H)-pyridazinones
Theochem 545:75-96.
Europe PMC
Simo M, Csampai A, Harmat V, Barabas O, Magyarfalvy G. (2001)
Novel ring transformations of condensed 1,2,4 triazolo- 4,3-b pyridazine-6(5H)-one-3(2H)-thiones effected by dialkyl-acetylenedicarboxylates
Tetrahedron 57(33):7191-7198.
Europe PMC
2000
Barabas O, Menyhard DK, Bocskei Z, Simon K, Kiss-Ajzert I, Takacs K, Hermecz I. (2000)
Structural basis of solid solution formation during chiral resolution
Tetrahedron Asymmetry 11(20):4061-4070.
Europe PMC