EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
Phone: +49 6221 387-8536
Biography
PhD, Max Planck Institute for Biophysical Chemistry, Göttingen.
Research Associate, the Scripps Research Institute, USA.
Group leader at EMBL since 2009.
Joint appointment with Cell Biology and Biophysics Unit.
Emmy Noether group leader since 2010.
ERC Investigator since 2015.
Publications
Total: 92 publication(s)
2020
Heinß N, Sushkin M, Yu M, Lemke EA. (2020)
Multifunctionality of F-rich nucleoporins.
Biochem Soc Trans 48(6) doi: 10.1042/BST20200357
Europe PMC | doi
Paci G, Zheng T, Caria J, Zilman A, Lemke EA. (2020)
Molecular determinants of large cargo transport into the nucleus.
Elife 9 doi: 10.7554/eLife.55963
Europe PMC | doi
Reinkemeier CD, Lemke EA. (2020)
Raising the ribosomal repertoire.
Nat Chem 12(6):503-504. doi: 10.1038/s41557-020-0476-6
Europe PMC | doi
Koehler C, Estrada Girona G, Reinkemeier CD, Lemke EA. (2020)
Inducible genetic code expansion in eukaryotes.
Chembiochem 21(22) doi: 10.1002/cbic.202000338
Europe PMC | doi
Celetti G, Paci G, Caria J, VanDelinder V, Bachand G, Lemke EA. (2020)
The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes.
J. Cell Biol. 219(1) doi: 10.1083/jcb.201907157
Europe PMC | doi
2019
Chowdhury A, Yu M, Lemke EA. (2019)
Phase separation comes of age: from phenomenology to single molecules.
Mol. Cell 74(3):413-415. doi: 10.1016/j.molcel.2019.04.016
Europe PMC | doi
Reinkemeier CD, Girona GE, Lemke EA. (2019)
Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes.
Science 363(6434) doi: 10.1126/science.aaw2644
Europe PMC | doi
Lu M, Ma X, Castillo-Menendez LR, Gorman J, Alsahafi N, Ermel U, Terry DS, Chambers M, Peng D, Zhang B, Zhou T, Reichard N, Wang K, Grover JR, Carman BP, Nikić-Spiegel I, Gardner MR, Arthos J, Sugawara A, Smith AB, Lemke EA, Abrams C, Farzan M, McDermott AB, Munro JB, Kwong PD, Finzi A, Sodroski JG, Blanchard SC, Mothes W. (2019)
Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET.
Nature 568(7752):415-419. doi: 10.1038/s41586-019-1101-y
Europe PMC | doi
Chowdhury A, Kovalenko SA, Aramburu IV, Tan PS, Ernsting NP, Lemke EA. (2019)
Mechanism dependent modulation of ultrafast interfacial water dynamics in intrinsically disordered protein complexes.
Angew. Chem. Int. Ed. Engl. 58(14) doi: 10.1002/anie.201813354
Europe PMC | doi
2018
Hellenkamp B, Schmid S, Doroshenko O, Opanasyuk O, Kühnemuth R, Adariani SR, Ambrose B, Aznauryan M, Barth A, Birkedal V, Bowen ME, Chen H, Cordes T, Eilert T, Fijen C, Götz M, Gebhardt C, Gratton E, Gouridis G, Hao P, Ha T, Hartmann A, Hanke CA, Hildebrandt LL, Hendrix J, Hohlbein J, Hirschfeld V, Hübner CG, Hua B, Kapanidis AN, Kallis E, Lamb DC, Lee NK, Kim JY, Krainer G, Levitus M, McCann JJ, Lemke EA, Levesque B, Ngo T, Qiu R, Naredi-Rainer N, Nettels D, Sanabria H, Schlierf M, Robb NC, Röcker C, Streit L, Seidel H, Schuler B, Schröder T, Vandenberk N, Tyagi S, Tinnefeld P, Thurn J, Yanez-Orozco IS, Wünsch B, Weninger KR, Vera AM, Hugel T, Craggs TD, Seidel CAM, Michaelis J. (2018)
Publisher Correction: Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study.
Nat. Methods 15(11) doi: 10.1038/s41592-018-0193-x
Europe PMC | doi
Hellenkamp B, Schmid S, Doroshenko O, Opanasyuk O, Kühnemuth R, Rezaei Adariani S, Ambrose B, Aznauryan M, Barth A, Birkedal V, Bowen ME, Chen H, Cordes T, Eilert T, Fijen C, Götz M, Gebhardt C, Gratton E, Gouridis G, Hao P, Ha T, Hartmann A, Hanke CA, Hildebrandt LL, Hendrix J, Hohlbein J, Hirschfeld V, Hübner CG, Hua B, Kapanidis AN, Kallis E, Lamb DC, Lee NK, Kim JY, Krainer G, Levitus M, McCann JJ, Lemke EA, Levesque B, Ngo T, Qiu R, Naredi-Rainer N, Nettels D, Sanabria H, Schlierf M, Robb NC, Röcker C, Streit L, Seidel H, Schuler B, Schröder T, Vandenberk N, Tyagi S, Tinnefeld P, Thurn J, Yanez-Orozco IS, Wünsch B, Weninger KR, Vera AM, Hugel T, Craggs TD, Seidel CAM, Michaelis J. (2018)
Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study.
Nat Methods 15(9):669-676. doi: 10.1038/s41592-018-0085-0
Europe PMC | doi
Tan PS, Lemke EA. (2018)
Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET.
Meth. Enzymol. 611:327-346. doi: 10.1016/bs.mie.2018.08.034
Europe PMC | doi
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Pappu RV, Svergun DI, Lemke EA. (2018)
Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water".
Science 361(6405) doi: 10.1126/science.aau8230
Europe PMC | doi
Wawryszyn M, Sauter PF, Nieger M, Koos MRM, Koehler C, Luy B, Lemke EA, Brase S. (2018)
Synthesis of Azido-Glycans for Chemical Glycomodification of Proteins
EUR J ORG CHEM 2018(31):4296-4305. doi: 10.1002/ejoc.201800602
doi
Das DK, Govindan R, Nikić-Spiegel I, Krammer F, Lemke EA, Munro JB. (2018)
Direct Visualization of the Conformational Dynamics of Single Influenza Hemagglutinin Trimers.
Cell 174(4) doi: 10.1016/j.cell.2018.05.050
Europe PMC | doi
Kormos A, Koehler C, Fodor EA, Rutkai ZR, Martin ME, Mező G, Lemke EA, Kele P. (2018)
Bistetrazine-Cyanines as Double-Clicking Fluorogenic Two-Point Binder or Crosslinker Probes.
Chemistry 24(35):8841-8847. doi: 10.1002/chem.201800910
Europe PMC | doi
Mikhaleva S, Lemke EA. (2018)
Beyond the Transport Function of Import Receptors: What's All the FUS about?
Cell 173(3):549-553. doi: 10.1016/j.cell.2018.04.002
Europe PMC | doi
Tan PS, Aramburu IV, Mercadante D, Tyagi S, Chowdhury A, Spitz D, Shammas SL, Gräter F, Lemke EA. (2018)
Two differential binding mechanisms of FG-nucleoporins and nuclear transport receptors.
Cell Rep 22(13):3660-3671. doi: 10.1016/j.celrep.2018.03.022
Europe PMC | doi
Brabham RL, Spears RJ, Walton J, Tyagi S, Lemke EA, Fascione MA. (2018)
Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification.
Chem Commun (Camb) 54(12):1501-1504. doi: 10.1039/c7cc07740h
Europe PMC | doi
Koehler C, Lemke EA. (2018)
MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells.
Methods Mol. Biol. 1728:297-311. doi: 10.1007/978-1-4939-7574-7_19
Europe PMC | doi
Kozma E, Paci G, Estrada Girona G, Lemke EA, Kele P. (2018)
Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins.
Methods Mol Biol 1728:337-363. doi: 10.1007/978-1-4939-7574-7_22
Europe PMC | doi
2017
Gupta K, Watson AA, Baptista T, Scheer E, Chambers AL, Koehler C, Zou J, Obong-Ebong I, Kandiah E, Temblador A, Round A, Forest E, Man P, Bieniossek C, Laue ED, Lemke EA, Rappsilber J, Robinson CV, Devys D, Tora L, Berger I. (2017)
Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID.
Elife 6 doi: 10.7554/eLife.30395
Europe PMC | doi
Warner JB, Ruff KM, Tan PS, Lemke EA, Pappu RV, Lashuel HA. (2017)
Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths.
J Am Chem Soc 139(41):14456-14469. doi: 10.1021/jacs.7b06659
Europe PMC | doi
Mercadante D, Wagner JA, Aramburu IV, Lemke EA, Gräter F. (2017)
Sampling Long- versus Short-Range Interactions Defines the Ability of Force Fields To Reproduce the Dynamics of Intrinsically Disordered Proteins.
J Chem Theory Comput 13(9):3964-3974. doi: 10.1021/acs.jctc.7b00143
Europe PMC | doi
Aramburu IV, Lemke EA. (2017)
Floppy but not sloppy: Interaction mechanism of FG-Nucleoporins and nuclear transport receptors.
Semin. Cell Dev. Biol. 68:34-41. doi: 10.1016/j.semcdb.2017.06.026
Europe PMC | doi
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA. (2017)
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351. doi: 10.1073/pnas.1704692114
Europe PMC | doi
Kozma E, Estrada Girona G, Paci G, Lemke EA, Kele P. (2017)
Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy.
Chem Commun (Camb) 53(50):6696-6699. doi: 10.1039/c7cc02212c
Europe PMC | doi
Sakin V, Hanne J, Dunder J, Anders-Össwein M, Laketa V, Nikić I, Kräusslich HG, Lemke EA, Müller B. (2017)
A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility.
Cell Chem Biol 24(5) doi: 10.1016/j.chembiol.2017.04.007
Europe PMC | doi
Demeter O, Kormos A, Koehler C, Mező G, Németh K, Kozma E, Takács LB, Lemke EA, Kele P. (2017)
Bisazide Cyanine Dyes as Fluorogenic Probes for Bis-Cyclooctynylated Peptide Tags and as Fluorogenic Cross-Linkers of Cyclooctynylated Proteins.
Bioconjug. Chem. 28(5):1552-1559. doi: 10.1021/acs.bioconjchem.7b00178
Europe PMC | doi
Kipper K, Lundius EG, Ćurić V, Nikić I, Wiessler M, Lemke EA, Elf J. (2017)
Application of Noncanonical Amino Acids for Protein Labeling in a Genomically Recoded Escherichia coli.
ACS Synth Biol 6(2):233-255. doi: 10.1021/acssynbio.6b00138
Europe PMC | doi
Kucher S, Korneev S, Tyagi S, Apfelbaum R, Grohmann D, Lemke EA, Klare JP, Steinhoff HJ, Klose D. (2017)
Orthogonal spin labeling using click chemistry for in vitro and in vivo applications.
J. Magn. Reson. 275:38-45. doi: 10.1016/j.jmr.2016.12.001
Europe PMC | doi
Paci G, Lemke EA. (2017)
Shining a Light on Phase Separation in the Cell.
Cell 168(1-2):11-13. doi: 10.1016/j.cell.2016.12.018
Europe PMC | doi
2016
Nikić I, Estrada Girona G, Kang JH, Paci G, Mikhaleva S, Koehler C, Shymanska NV, Ventura Santos C, Spitz D, Lemke EA. (2016)
Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy.
Angew. Chem. Int. Ed. Engl. 55(52):16172-16176. doi: 10.1002/anie.201608284
Europe PMC | doi
Koehler C, Sauter PF, Wawryszyn M, Girona GE, Gupta K, Landry JJ, Fritz MH, Radic K, Hoffmann JE, Chen ZA, Zou J, Tan PS, Galik B, Junttila S, Stolt-Bergner P, Pruneri G, Gyenesei A, Schultz C, Biskup MB, Besir H, Benes V, Rappsilber J, Jechlinger M, Korbel JO, Berger I, Braese S, Lemke EA. (2016)
Genetic code expansion for multiprotein complex engineering.
Nat. Methods 13(12) doi: 10.1038/nmeth.4032
Europe PMC | doi
Kozma E, Nikic I, Varga BR, Aramburu IV, Kang JH, Fackler OT, Lemke EA, Kele P. (2016)
Hydrophilic trans-Cyclooctenylated Noncanonical Amino Acids for Fast Intracellular Protein Labeling
Chembiochem 17(16):1518-1524. doi: 10.1002/cbic.201600284
Europe PMC | doi
Knorr G, Kozma E, Herner A, Lemke EA, Kele P. (2016)
New Red-Emitting Tetrazine-Phenoxazine Fluorogenic Labels for Live-Cell Intracellular Bioorthogonal Labeling Schemes.
Chemistry 22(26):8972-8979. doi: 10.1002/chem.201600590
Europe PMC | doi
Banterle N, Lemke EA. (2016)
Nanoscale devices for linkerless long-term single-molecule observation.
Curr. Opin. Biotechnol. 39:105-112. doi: 10.1016/j.copbio.2016.02.013
Europe PMC | doi
Sakin V, Paci G, Lemke EA, Müller B. (2016)
Labeling of virus components for advanced, quantitative imaging analyses.
FEBS Lett. 590(13) doi: 10.1002/1873-3468.12131
Europe PMC | doi
Lemke EA. (2016)
The Multiple Faces of Disordered Nucleoporins.
J Mol Biol 428(10) doi: 10.1016/j.jmb.2016.01.002
Europe PMC | doi
2015
Vreja IC, Nikić I, Göttfert F, Bates M, Kröhnert K, Outeiro TF, Hell SW, Lemke EA, Rizzoli SO. (2015)
Super-resolution Microscopy of Clickable Amino Acids Reveals the Effects of Fluorescent Protein Tagging on Protein Assemblies.
ACS Nano 9(11):11034-11041. doi: 10.1021/acsnano.5b04434
Europe PMC | doi
Milles S, Mercadante D, Aramburu IV, Jensen MR, Banterle N, Koehler C, Tyagi S, Clarke J, Shammas SL, Blackledge M, Gräter F, Lemke EA. (2015)
Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors.
Cell 163(3) doi: 10.1016/j.cell.2015.09.047
Europe PMC | doi
Delaforge E, Milles S, Bouvignies G, Bouvier D, Boivin S, Salvi N, Maurin D, Martel A, Round A, Lemke EA, Jensen MR, Hart D, Blackledge M. (2015)
Large Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α.
J. Am. Chem. Soc. 137(48) doi: 10.1021/jacs.5b07765
Europe PMC | doi
von Appen A, Kosinski J, Sparks L, Ori A, DiGuilio AL, Vollmer B, Mackmull MT, Banterle N, Parca L, Kastritis P, Buczak K, Mosalaganti S, Hagen W, Andres-Pons A, Lemke EA, Bork P, Antonin W, Glavy JS, Bui KH, Beck M. (2015)
In situ structural analysis of the human nuclear pore complex.
Nature 526(7571):140-143. doi: 10.1038/nature15381
Europe PMC | doi
Hoffmann JE, Plass T, Nikić I, Aramburu IV, Koehler C, Gillandt H, Lemke EA, Schultz C. (2015)
Highly Stable trans-Cyclooctene Amino Acids for Live-Cell Labeling.
Chem-Eur J 21(35):12266-12270. doi: 10.1002/chem.201501647
Europe PMC | doi
Wagner JA, Mercadante D, Nikić I, Lemke EA, Grater F. (2015)
Origin of Orthogonality of Strain-Promoted Click Reactions.
Chem-Eur J 21(35):12431-12435. doi: 10.1002/chem.201501727
Europe PMC | doi
Hoffmann JE, Plass T, Nikić I, Aramburu IV, Koehler C, Gillandt H, Lemke EA, Schultz C. (2015)
Frontispiece: Highly Stable trans-Cyclooctene Amino Acids for Live-Cell Labeling.
Chemistry 21(35) doi: 10.1002/chem.201583561
Europe PMC | doi
Nikić I, Lemke EA. (2015)
Genetic code expansion enabled site-specific dual-color protein labeling: superresolution microscopy and beyond.
Curr Opin Chem Biol 28:164-173. doi: 10.1016/j.cbpa.2015.07.021
Europe PMC | doi
Mercadante D, Milles S, Fuertes G, Svergun DI, Lemke EA, Gräter F. (2015)
Kirkwood-Buff Approach Rescues Overcollapse of a Disordered Protein in Canonical Protein Force Fields
J Phys Chem B 119(25):7975-7984. doi: 10.1021/acs.jpcb.5b03440
Europe PMC | doi
Nikić I, Kang JH, Girona GE, Aramburu IV, Lemke EA. (2015)
Labeling proteins on live mammalian cells using click chemistry.
Nat Protoc 10(5):780-791. doi: 10.1038/nprot.2015.045
Europe PMC | doi
Tyagi S, Lemke EA. (2015)
Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids.
Curr. Opin. Struct. Biol. 32:66-73. doi: 10.1016/j.sbi.2015.02.009
Europe PMC | doi
2014
Lemke EA. (2014)
Expanding the Genetic Code.
Chembiochem 15(12):1691-1694. doi: 10.1002/cbic.201402362
Europe PMC | doi
Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R. (2014)
The CD27L and CTP1L Endolysins Targeting Clostridia Contain a Built-in Trigger and Release Factor
PLoS Pathog. 10(7):e1004228. doi: 10.1371/journal.ppat.1004228
Europe PMC | doi
Pavic K, Rios P, Dzeyk K, Koehler C, Lemke EA, Köhn M. (2014)
Unnatural Amino Acid Mutagenesis Reveals Dimerization As a Negative Regulatory Mechanism of VHR's Phosphatase Activity
ACS Chem. Biol. 9(7):1451-1459. doi: 10.1021/cb500240n
Europe PMC | doi
Milles S, Lemke EA. (2014)
Mapping Multivalency and Differential Affinities within Large Intrinsically Disordered Protein Complexes with Segmental Motion Analysis.
Angew. Chem. Int. Ed. Engl. 53(28):7364-7367. doi: 10.1002/anie.201403694
Europe PMC | doi
Herner A, Estrada Girona G, Nikic I, Kallay M, Lemke EA, Kele P. (2014)
New generation of bioorthogonally applicable fluorogenic dyes with visible excitations and large Stokes-shifts.
Bioconjug. Chem. 25(7):1370-1374. doi: 10.1021/bc500235p
Europe PMC | doi
Tyagi S, Vandelinder V, Banterle N, Fuertes G, Milles S, Agez M, Lemke EA. (2014)
Continuous throughput and long-term observation of single-molecule FRET without immobilization
Nat. Methods 11(3):297-300. doi: 10.1038/nmeth.2809
Europe PMC | doi
Nikić I, Plass T, Schraidt O, Szymański J, Briggs JA, Schultz C, Lemke EA. (2014)
Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy
Angew. Chem. Int. Ed. Engl. 53(8):2245-2249. doi: 10.1002/anie.201309847
Europe PMC | doi
2013
Banterle N, Bui KH, Lemke EA, Beck M. (2013)
Fourier ring correlation as a resolution criterion for super-resolution microscopy
J. Struct. Biol. 183(3):363-367. doi: 10.1016/j.jsb.2013.05.004
Europe PMC | doi
Herner A, Nikić I, Kállay M, Lemke EA, Kele P. (2013)
A new family of bioorthogonally applicable fluorogenic labels
Org. Biomol. Chem. 11(20):3297-3306. doi: 10.1039/c3ob40296g
Europe PMC | doi
Lukinavičius G, Umezawa K, Olivier N, Honigmann A, Yang G, Plass T, Mueller V, Reymond L, Corrêa IR Jr, Luo ZG, Schultz C, Lemke EA, Heppenstall P, Eggeling C, Manley S, Johnsson K. (2013)
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
Nat Chem 5(2):132-139. doi: 10.1038/nchem.1546
Europe PMC | doi
Milles S, Huy Bui K, Koehler C, Eltsov M, Beck M, Lemke EA. (2013)
Facilitated aggregation of FG nucleoporins under molecular crowding conditions
EMBO Rep. 14(2):178-183. doi: 10.1038/embor.2012.204
Europe PMC | doi
Milles S, Lemke EA. (2013)
Nup153 in Nuclear Transport: Plasticity of Nucleoporin/Transport-Receptor Complexes
BIOPHYS J 104(2):30A. doi: 10.1016/j.bpj.2012.11.202
doi
Milles S, Tyagi S, Plass T, Koehler C, Schultz C, Lemke EA. (2013)
Labeling Freedom for the Single Molecule Microscopist
BIOPHYS J 104(2):576A. doi: 10.1016/j.bpj.2012.11.3197
doi
Tyagi S, Vandelinder V, Banterle N, Vives GF, Agez M, Milles S, Lemke EA. (2013)
Long-Term Single-Molecule TIRF Observation of Biomolecules without Immobilization
BIOPHYS J 104(2):174A. doi: 10.1016/j.bpj.2012.11.980
doi
Milles S, Lemke EA. (2013)
What precision-protein-tuning and nano-resolved single molecule sciences can do for each other
Bioessays 35(1):65-74. doi: 10.1002/bies.201200094
Europe PMC | doi
Ori A, Banterle N, Iskar M, Andrés-Pons A, Escher C, Khanh Bui H, Sparks L, Solis-Mezarino V, Rinner O, Bork P, Lemke EA, Beck M. (2013)
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines
Mol. Syst. Biol. 9:648. doi: 10.1038/msb.2013.4
Europe PMC | doi
Tyagi S, Lemke EA. (2013)
Genetically encoded click chemistry for single-molecule FRET of proteins
Methods Cell Biol. 113:169-187. doi: 10.1016/b978-0-12-407239-8.00009-4
Europe PMC | doi
2012
Milles S, Koehler C, Gambin Y, Deniz AA, Lemke EA. (2012)
Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range
Mol Biosyst 8(10):2531-2534. doi: 10.1039/c2mb25135c
Europe PMC | doi
Borrmann A, Milles S, Plass T, Dommerholt J, Verkade JM, Wiessler M, Schultz C, van Hest JC, van Delft FL, Lemke EA. (2012)
Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation
Chembiochem 13(14):2094-2099. doi: 10.1002/cbic.201200407
Europe PMC | doi
Krishnan R, Goodman JL, Mukhopadhyay S, Pacheco CD, Lemke EA, Deniz AA, Lindquist S. (2012)
Conserved features of intermediates in amyloid assembly determine their benign or toxic states
Proc. Natl. Acad. Sci. U.S.A. 109(28):11172-11177. doi: 10.1073/pnas.1209527109
Europe PMC | doi
Plass T, Milles S, Koehler C, Szymański J, Mueller R, Wiessler M, Schultz C, Lemke EA. (2012)
Amino acids for Diels-Alder reactions in living cells
Angew. Chem. Int. Ed. Engl. 51(17):4166-4170. doi: 10.1002/anie.201108231
Europe PMC | doi
Milles S, Tyagi S, Banterle N, Koehler C, VanDelinder V, Plass T, Neal AP, Lemke EA. (2012)
Click strategies for single-molecule protein fluorescence
J. Am. Chem. Soc. 134(11):5187-5195. doi: 10.1021/ja210587q
Europe PMC | doi
Plass T, Milles S, Koehler C, Schultz C, Lemke EA. (2012)
Labeling Freedom for the Single Molecule Microscopist
Biophys J 102(3 Suppl 1):217A. doi: 10.1016/j.bpj.2011.11.1187
doi
2011
Milles S, Lemke EA. (2011)
Single molecule study of the intrinsically disordered FG-repeat nucleoporin 153
Biophys. J. 101(7):1710-1719. doi: 10.1016/j.bpj.2011.08.025
Europe PMC | doi
Lemke EA, Schultz C. (2011)
Principles for designing fluorescent sensors and reporters
Nat. Chem. Biol. 7(8):480-483. doi: 10.1038/nchembio.620
Europe PMC | doi
Plass T, Milles S, Koehler C, Schultz C, Lemke EA. (2011)
Genetically encoded copper-free click chemistry
Angew. Chem. Int. Ed. Engl. 50(17):3878-3881. doi: 10.1002/anie.201008178
Europe PMC | doi
Gambin Y, VanDelinder V, Ferreon AC, Lemke EA, Groisman A, Deniz AA. (2011)
Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing
Nat. Methods 8(3):239-241. doi: 10.1038/nmeth.1568
Europe PMC | doi
Lemke EA. (2011)
Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities
Methods Mol. Biol. 751:3-15. doi: 10.1007/978-1-61779-151-2_1
Europe PMC | doi
2010
Lemke EA. (2010)
Precision control of cellular pathways with light
Chembiochem 11(13):1825-1827. doi: 10.1002/cbic.201000364
Europe PMC | doi
2009
Lee HS, Guo J, Lemke EA, Dimla RD, Schultz PG. (2009)
Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae
J. Am. Chem. Soc. 131(36):12921-12923. doi: 10.1021/ja904896s
Europe PMC | doi
Lemke EA, Gambin Y, Vandelinder V, Brustad EM, Liu HW, Schultz PG, Groisman A, Deniz AA. (2009)
Microfluidic device for single-molecule experiments with enhanced photostability
J. Am. Chem. Soc. 131(38):13610-13612. doi: 10.1021/ja9027023
Europe PMC | doi
Gambin Y, Schug A, Lemke EA, Lavinder JJ, Ferreon AC, Magliery TJ, Onuchic JN, Deniz AA. (2009)
Direct single-molecule observation of a protein living in two opposed native structures
Proc. Natl. Acad. Sci. U.S.A. 106(25):10153-10158. doi: 10.1073/pnas.0904461106
Europe PMC | doi
Ferreon AC, Gambin Y, Lemke EA, Deniz AA. (2009)
Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence
Proc. Natl. Acad. Sci. U.S.A. 106(14):5645-5650. doi: 10.1073/pnas.0809232106
Europe PMC | doi
2008
Brustad EM, Lemke EA, Schultz PG, Deniz AA. (2008)
A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer
J. Am. Chem. Soc. 130(52):17664-17665. doi: 10.1021/ja807430h
Europe PMC | doi
Deniz AA, Mukhopadhyay S, Lemke EA. (2008)
Single-molecule biophysics: at the interface of biology, physics and chemistry
J R Soc Interface 5(18):15-45. doi: 10.1098/rsif.2007.1021
Europe PMC | doi
2007
Lemke EA, Summerer D, Geierstanger BH, Brittain SM, Schultz PG. (2007)
Control of protein phosphorylation with a genetically encoded photocaged amino acid
Nat. Chem. Biol. 3(12):769-772. doi: 10.1038/nchembio.2007.44
Europe PMC | doi
Henkel AW, Dittrich PS, Groemer TW, Lemke EA, Klingauf J, Klafki HW, Lewczuk P, Esselmann H, Schwille P, Kornhuber J, Wiltfang J. (2007)
Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients
Mol. Psychiatry 12(6):601-610. doi: 10.1038/sj.mp.4001947
Europe PMC | doi
Mukhopadhyay S, Krishnan R, Lemke EA, Lindquist S, Deniz AA. (2007)
A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures
Proc. Natl. Acad. Sci. U.S.A. 104(8):2649-2654. doi: 10.1073/pnas.0611503104
Europe PMC | doi
2006
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, Rammner B, Gräter F, Hub JS, De Groot BL, Mieskes G, Moriyama Y, Klingauf J, Grubmüller H, Heuser J, Wieland F, Jahn R. (2006)
Molecular anatomy of a trafficking organelle
Cell 127(4):831-846. doi: 10.1016/j.cell.2006.10.030
Europe PMC | doi
2005
Lemke EA, Klingauf J. (2005)
Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity
J. Neurosci. 25(47):11034-11044. doi: 10.1523/jneurosci.2971-05.2005
Europe PMC | doi
Jordan R, Lemke EA, Klingauf J. (2005)
Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy
Biophys. J. 89(3):2091-2102. doi: 10.1529/biophysj.105.061663
Europe PMC | doi
2003
Chakraborty R, Lemke EA, Cao Z, Klebba PE, van der Helm D. (2003)
Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli
Biometals 16(4):507-518. doi: 10.1023/a:1023485632520
Europe PMC | doi