EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany


  • PhD 2007, University of Jena/FLI-Leibniz-Institute for Age Research and Brandeis University, Waltham, Massachusetts.
    Postdoctoral research at Max Planck Research Unit for Enzymology of Protein Folding, Halle, and at MRC Laboratory of Molecular Biology, Cambridge.
    Group leader at EMBL since 2010.


Total: 45 publication(s)


Tarafder AK, Guesdon A, Kuhm T, Sachse C. (2019)

Recombinant Expression, Purification, and Assembly of p62 Filaments.

Methods Mol. Biol. 1880:3-15. doi: 10.1007/978-1-4939-8873-0_1
Europe PMC | doi

Beckers M, Jakobi AJ, Sachse C. (2019)

Thresholding of cryo-EM density maps by false discovery rate control.

IUCrJ 6(Pt 1):18-33. doi: 10.1107/S2052252518014434
Europe PMC | doi


Pellegrini E, Desfosses A, Wallmann A, Schulze WM, Rehbein K, Mas P, Signor L, Gaudon S, Zenkeviciute G, Hons M, Malet H, Gutsche I, Sachse C, Schoehn G, Oschkinat H, Cusack S. (2018)

RIP2 filament formation is required for NOD2 dependent NF-κB signalling.

Nat Commun 9(1) doi: 10.1038/s41467-018-06451-3
Europe PMC | doi

Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S. (2018)

Phasing out the bad-How SQSTM1/p62 sequesters ubiquitinated proteins for degradation by autophagy.

Autophagy 14(7) doi: 10.1080/15548627.2018.1462079
Europe PMC | doi

Huber ST, Kuhm T, Sachse C. (2018)

Automated tracing of helical assemblies from electron cryo-micrographs.

J. Struct. Biol. 202(1) doi: 10.1016/j.jsb.2017.11.013
Europe PMC | doi

Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S. (2018)

p62 filaments capture and present ubiquitinated cargos for autophagy.

EMBO J 37(5) doi: 10.15252/embj.201798308
Europe PMC | doi


Jakobi AJ, Wilmanns M, Sachse C. (2017)

Model-based local density sharpening of cryo-EM maps.

Elife 6 doi: 10.7554/eLife.27131
Europe PMC | doi

Sadian Y, Tafur L, Kosinski J, Jakobi AJ, Wetzel R, Buczak K, Hagen WJ, Beck M, Sachse C, Müller CW. (2017)

Structural insights into transcription initiation by yeast RNA polymerase I.

EMBO J 36(18) doi: 10.15252/embj.201796958
Europe PMC | doi

Kolovou A, Schorb M, Tarafder A, Sachse C, Schwab Y, Santarella-Mellwig R. (2017)

A new method for cryo-sectioning cell monolayers using a correlative workflow.

Methods Cell Biol 140:85-103. doi: 10.1016/bs.mcb.2017.03.011
Europe PMC | doi


Tafur L, Sadian Y, Hoffmann NA, Jakobi AJ, Wetzel R, Hagen WJ, Sachse C, Müller CW. (2016)

Molecular Structures of Transcribing RNA Polymerase I

Mol Cell. 64(6):1135-1143. doi: 10.1016/j.molcel.2016.11.013
Europe PMC | doi

Dauden MI, Kosinski J, Kolaj-Robin O, Desfosses A, Ori A, Faux C, Hoffmann NA, Onuma OF, Breunig KD, Beck M, Sachse C, Séraphin B, Glatt S, Müller CW. (2016)

Architecture of the yeast Elongator complex

EMBO Rep. 18(2):e201643353. doi: 10.15252/embr.201643353
Europe PMC | doi

Ohashi Y, Soler N, García Ortegón M, Zhang L, Kirsten ML, Perisic O, Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi M, Ktistakis NT, Sachse C, Williams RL. (2016)

Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex.

Autophagy 12(11):2129-2144. doi: 10.1080/15548627.2016.1226736
Europe PMC | doi

Hoffmann NA, Jakobi AJ, Vorländer MK, Sachse C, Müller CW. (2016)

Transcribing RNA polymerase III observed by electron cryo-microscopy.

FEBS J. 283(15):2811-2819. doi: 10.1111/febs.13732
Europe PMC | doi

Schur FK, Obr M, Hagen WJ, Wan W, Jakobi AJ, Kirkpatrick JM, Sachse C, Kräusslich HG, Briggs JA. (2016)

An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation.

Science 353(6298):506-508. doi: 10.1126/science.aaf9620
Europe PMC | doi

Bertipaglia C, Schneider S, Jakobi AJ, Tarafder AK, Bykov YS, Picco A, Kukulski W, Kosinski J, Hagen WJ, Ravichandran AC, Wilmanns M, Kaksonen M, Briggs JA, Sachse C. (2016)

Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle.

EMBO Rep. 17(7):1044-1060. doi: 10.15252/embr.201541960
Europe PMC | doi

Klionsky DJ et al. (2016)

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).

Autophagy 12(1):1-222. doi: 10.1080/15548627.2015.1100356
Europe PMC | doi

Fromm SA, Sachse C. (2016)

Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction

Meth. Enzymol. 579:307-328. doi: 10.1016/bs.mie.2016.05.034
Europe PMC | doi


Hoffmann NA, Jakobi AJ, Moreno-Morcillo M, Glatt S, Kosinski J, Hagen WJ, Sachse C, Müller CW. (2015)

Molecular structures of unbound and transcribing RNA polymerase III.

Nature 528(7581):231-236. doi: 10.1038/nature16143
Europe PMC | doi

Johansen T, Sachse C. (2015)

The higher-order molecular organization of p62/SQSTM1.

Oncotarget 6(19):16796-16797. doi: 10.18632/oncotarget.4590
Europe PMC | doi

Sachse C. (2015)

Single-particle based helical reconstruction – how to make the most of real and Fourier space

AIMS Biophys 2(2):219-244. doi: 10.3934/biophy.2015.2.219

Ciuffa R, Lamark T, Tarafder AK, Guesdon A, Rybina S, Hagen WJ, Johansen T, Sachse C. (2015)

The Selective Autophagy Receptor p62 Forms a Flexible Filamentous Helical Scaffold.

Cell Rep 11(5):748-758. doi: 10.1016/j.celrep.2015.03.062
Europe PMC | doi

Bharat TA, Murshudov GN, Sachse C, Löwe J. (2015)

Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.

Nature 523(7558):1-5. doi: 10.1038/nature14356
Europe PMC | doi

Skruzny M, Desfosses A, Prinz S, Dodonova SO, Gieras A, Uetrecht C, Jakobi AJ, Abella M, Hagen WJ, Schulz J, Meijers R, Rybin V, Briggs JA, Sachse C, Kaksonen M. (2015)

An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis.

Dev. Cell 33(2):150-162. doi: 10.1016/j.devcel.2015.02.023
Europe PMC | doi

Gutsche I, Desfosses A, Effantin G, Ling WL, Haupt M, Ruigrok RW, Sachse C, Schoehn G. (2015)

Near-atomic cryo-EM structure of the helical measles virus nucleocapsid.

Science 348(6235):704-707. doi: 10.1126/science.aaa5137
Europe PMC | doi

Ehebauer MT, Zimmermann M, Jakobi AJ, Noens EE, Laubitz D, Cichocki B, Marrakchi H, Lanéelle MA, Daffé M, Sachse C, Dziembowski A, Sauer U, Wilmanns M. (2015)

Characterization of the Mycobacterial Acyl-CoA Carboxylase Holo Complexes Reveals Their Functional Expansion into Amino Acid Catabolism.

PLoS Pathog. 11(2) doi: 10.1371/journal.ppat.1004623
Europe PMC | doi

Elad N, De Strooper B, Lismont S, Hagen W, Veugelen S, Arimon M, Horré K, Berezovska O, Sachse C, Chávez-Gutiérrez L. (2015)

The dynamic conformational landscape of γ-secretase.

Journal of Cell Science 128(3):589-598. doi: 10.1242/jcs.164384
Europe PMC | doi

Fromm SA, Bharat TA, Jakobi AJ, Hagen WJ, Sachse C. (2015)

Seeing tobacco mosaic virus through direct electron detectors.

J. Struct. Biol. 189(2):87-97. doi: 10.1016/j.jsb.2014.12.002
Europe PMC | doi


Afonin S, Glaser RW, Sachse C, Salgado J, Wadhwani P, Ulrich AS. (2014)

(19)F NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids

Biochim. Biophys. Acta 1838(9):2260-2268. doi: 10.1016/j.bbamem.2014.03.017
Europe PMC | doi

Vahokoski J, Bhargav SP, Desfosses A, Andreadaki M, Kumpula EP, Martinez SM, Ignatev A, Lepper S, Frischknecht F, Sidén-Kiamos I, Sachse C, Kursula I. (2014)

Structural differences explain diverse functions of Plasmodium actins.

PLoS Pathog. 10(4) doi: 10.1371/journal.ppat.1004091
Europe PMC | doi

Desfosses A, Ciuffa R, Gutsche I, Sachse C. (2014)

SPRING - an image processing package for single-particle based helical reconstruction from electron cryomicrographs

J. Struct. Biol. 185(1):15-26. doi: 10.1016/j.jsb.2013.11.003
Europe PMC | doi


Gombos L, Neuner A, Berynskyy M, Fava LL, Wade RC, Sachse C, Schiebel E. (2013)

GTP regulates the microtubule nucleation activity of γ-tubulin

Nat. Cell Biol. 15(11):1317-1327. doi: 10.1038/ncb2863
Europe PMC | doi


Guichard P, Desfosses A, Maheshwari A, Hachet V, Dietrich C, Brune A, Ishikawa T, Sachse C, Gönczy P. (2012)

Cartwheel architecture of Trichonympha basal body

Science 337(6094):553. doi: 10.1126/science.1222789
Europe PMC | doi

Bharat TA, Davey NE, Ulbrich P, Riches JD, de Marco A, Rumlova M, Sachse C, Ruml T, Briggs JA. (2012)

Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy

Nature 487(7407):385-389. doi: 10.1038/nature11169
Europe PMC | doi


Korkhov VM, Sachse C, Short JM, Tate CG. (2010)

Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals

Structure 18(6):677-687. doi: 10.1016/j.str.2010.03.001
Europe PMC | doi

Sachse C, Grigorieff N, Fändrich M. (2010)

Nanoscale flexibility parameters of Alzheimer amyloid fibrils determined by electron cryo-microscopy

Angew. Chem. Int. Ed. Engl. 49(7):1321-1323. doi: 10.1002/anie.200904781
Europe PMC | doi


Low HH, Sachse C, Amos LA, Löwe J. (2009)

Structure of a bacterial dynamin-like protein lipid tube provides a mechanism for assembly and membrane curving

Cell 139(7):1342-1352. doi: 10.1016/j.cell.2009.11.003
Europe PMC | doi

Schmidt M, Sachse C, Richter W, Xu C, Fändrich M, Grigorieff N. (2009)

Comparison of Alzheimer Abeta(1-40) and Abeta(1-42) amyloid fibrils reveals similar protofilament structures

Proc. Natl. Acad. Sci. U.S.A. 106(47):19813-19818. doi: 10.1073/pnas.0905007106
Europe PMC | doi

Meinhardt J, Sachse C, Hortschansky P, Grigorieff N, Fändrich M. (2009)

Abeta(1-40) fibril polymorphism implies diverse interaction patterns in amyloid fibrils

J. Mol. Biol. 386(3):869-877. doi: 10.1016/j.jmb.2008.11.005
Europe PMC | doi


Sachse C, Fändrich M, Grigorieff N. (2008)

Paired beta-sheet structure of an Abeta(1-40) amyloid fibril revealed by electron microscopy

Proc. Natl. Acad. Sci. U.S.A. 105(21):7462-7466. doi: 10.1073/pnas.0712290105
Europe PMC | doi

Chen JZ, Sachse C, Xu C, Mielke T, Spahn CM, Grigorieff N. (2008)

A dose-rate effect in single-particle electron microscopy

J. Struct. Biol. 161(1):92-100. doi: 10.1016/j.jsb.2007.09.017
Europe PMC | doi


Habicht G, Haupt C, Friedrich RP, Hortschansky P, Sachse C, Meinhardt J, Wieligmann K, Gellermann GP, Brodhun M, Götz J, Halbhuber KJ, Röcken C, Horn U, Fändrich M. (2007)

Directed selection of a conformational antibody domain that prevents mature amyloid fibril formation by stabilizing Abeta protofibrils

Proc. Natl. Acad. Sci. U.S.A. 104(49):19232-19237. doi: 10.1073/pnas.0703793104
Europe PMC | doi

Sachse C, Chen JZ, Coureux PD, Stroupe ME, Fändrich M, Grigorieff N. (2007)

High-resolution electron microscopy of helical specimens: a fresh look at tobacco mosaic virus

J. Mol. Biol. 371(3):812-835. doi: 10.1016/j.jmb.2007.05.088
Europe PMC | doi


Sachse C, Xu C, Wieligmann K, Diekmann S, Grigorieff N, Fändrich M. (2006)

Quaternary structure of a mature amyloid fibril from Alzheimer's Abeta(1-40) peptide

J. Mol. Biol. 362(2):347-354. doi: 10.1016/j.jmb.2006.07.011
Europe PMC | doi


Glaser RW, Sachse C, Dürr UH, Wadhwani P, Afonin S, Strandberg E, Ulrich AS. (2005)

Concentration-dependent realignment of the antimicrobial peptide PGLa in lipid membranes observed by solid-state 19F-NMR

Biophys. J. 88(5):3392-3397. doi: 10.1529/biophysj.104.056424
Europe PMC | doi


Glaser RW, Sachse C, Dürr UH, Wadhwani P, Ulrich AS. (2004)

Orientation of the antimicrobial peptide PGLa in lipid membranes determined from 19F-NMR dipolar couplings of 4-CF3-phenylglycine labels

J. Magn. Reson. 168(1):153-163. doi: 10.1016/j.jmr.2004.02.008
Europe PMC | doi