Widefield Microscopes

Microscope Features Room
Nikon Ti-E
book details
Motorized xy-stage, perfect focus system (PFS) hardware autofocus, Lumencore spectraX, emission filter wheel, sCMOS PCO edge 4.2 CL 13-514A
Olympus Cell^R TIRF
book details
EMCCD, multi-color TIRF (405, 488, 561, 640 nm laser), Environment box (cellVivo), filterwheels, motorized xy-stage, hardware autofocus (ZDC3) 13-515E
Olympus LV200
book details
bioluminescence and fluorescence detection, light shielding environment box, 13-515B
Zeiss Cellobserver
book details
Environment box, metal halide fluorescence lamp, motorized xy-stage 13-515D
Zeiss MicroBeam
book details
Laser microdissection microscope system, collection of samples by laser catapulting. 13-512
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Confocal Microscopes

Microscope Features Room
Leica SP5 (C)
book details
Confocal laser scanning microscope. available laser wavelengths: 405, 488, 560, 640 nm, motorized xy-stage, 3 HyDs, no incubation control 13-514C
Leica SP8 STED 3X
book details
Confocal laser scanning microscope with white-light laser (470-670 nm) and gated detection on two HyD detectors,
stimulated emission depletion (STED) 3D super-resolution (592, 660 and 775 nm STED lasers), environment box, motorized xy-stage
13-516B

Leica SP8 DLS
book details

Confocal laser scanning microscope with light sheet option (SPIM). available laser wavelengths: 405, 488, 552, 640 nm, motorized xy-stage 13-509C
Olympus FV1200
book details
Confocal laser scanning microscope with two scanners, second scanner with pulsed lasers (355, 375, 532 nm) for nano-surgery and photo-uncagingFRAP, FRET, photo-activation, environment box, hardware autofocus (ZDC3) 13-516E
Olympus FV3000
book details
Confocal laser scanning microscope with 4 GaAsP spectral detectors, FRAP, FRET lasers: 405, 445, 488, 514, 561, 594, 640 nm, environment box, hardware autofocus (ZDC), motorized xy-stage 13-514E
PE Ultraview ERS
book details
Spinning disk confocal microscope, FRET, lasers: 405, 488, 514, 561, 640 nm, environment box, motorized xy-stage
13-508D
PE Ultraview VoX
book details
Spinning disk confocal microscope, photokinesis unit (FRAP, photo-activation), FRET, environment box, lasers: 405, 440, 488, 514, 561, 640 nm, motorized xy-stage 13-514D
Zeiss LSM 780
book details
Confocal laser scanning microscope with GaAsP spectral detector, FCS, FRAP, FRET lasers: 405, 458, 488, 514, 561, 633 nm, environment box, hardware autofocus (definite focus), motorized xy-stage 13-516C
Zeiss LSM 780 NLO
book details
2-photon (can be used for nano-surgery and photo-uncaging) and confocal laser scanning microscope, FCS, FRAP, FRET,  GaAsP external detectors, spectral GaAsP detection, lasers: 405, 458, 488, 514, 561, 594, 633 nm, 690-1064 nm(2-photon laser), environment box, hardware autofocus (definite focus), motorized xy-stage 13-516D
Zeiss LSM 880 AIRY fast
book details
Confocal laser scanning microscope with AIRY fast module. The AIRY fast module allows faster acquisition even with increased resolution compared to normal confocal scanning. 13-514B
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Lightsheet Microscopes

Microscope Features Room

Leica SP8 DLS
book details

Confocal laser scanning microscope with light sheet option.  „Inverted“ light sheet microscope with two independent opposing light sheets and one detection objective. The system is equipped with an EMBL incubation box. Available magnifications and Laser lines are the following: 16x; 40x; 405nm; 488nm; 552nm; 638nm. The system is best for imaging thicker samples which can’t be mounted in a FEP tube or capillary and need environmental control.

13-509C
Luxendo MuVi SPIM
book details
Light sheet microscope with parallel illumination from two sides and parallel detection from two sides perpendicular to the illumination. Possibility of sample rotation offers the opportunity to improve axial resolution and imaging quality after image. Available magnifications and Laser lines are the following: 33.3x; 22.2x;445nm; 488nm; 515nm; 561nm; 642nm. The system is best for imaging Drosophila, Zebrafish and thicker samples, which do not need CO2 control and can be mounted in a capillary or FEP tube. 13-509B
Luxendo InVi SPIM
book details

„Inverted“ light sheet microscope with 2 cameras for simultaneous dual color acquisition. The system is equipped with temperature- and humidity control and a gas mixer to control CO2 and O2 levels. Available magnifications and Laser lines are the following: 31.25x; 62.5x; 445nm; 488nm; 515nm; 561nm; 594nm; 642nm. The system has one detection and one illumination objective and is best for small transparent samples, which are sensitive, difficult to mount and need environmental control.

13-509A
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Super-Resolution Microscopes

Microscope Features Room
Abberior RESOLFT
book details
Combination of RESOLFT (super-resolution with switchable FPs) and STED (775 nm depletion laser) microscope. Motorized xy/stage. 13-516A
Leica GSDIM
book details
Localization based super-resolution in 3D by ground state depletion by individual return microscopy (GSDIM, also called dSTORM) 13-509D
Leica SP8 STED 3X
book details
3D stimulated emission depletion (STED) microscopy with gated detection, environment box, motorized xy-stage, lasers: 405 nm, Ar-laser (458, 476, 488, 496, 514 nm), white-light laser (470-670 nm), STED laser (592, 660 + 775 nm) 13-516B
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High-throughput microscopy

Microscope Features Room

Leica SP5 MSA (A)
book
 details

Confocal laser scanning microscope, environment box, MSA, adaptive feedback microscopy, EMBL dispenser 13-508F

Leica SP5 MSA (B)
book
 details

Confocal laser scanning microscope, environment box, MSA, adaptive feedback microscopy, EMBL dispenser 13-508E
Molecular Devices IXM
book details
Widefield epifluorescence and transmission microscope, sCMOS camera, Environment box, automated pipetting 13-508B
Nikon Ti-E PLateloader
book details
Widefield epifluorescence and transmission microscope, hardware autofocus (PFS) and plateloader to autmatically load 96- and 384-well plates 13-508B
Olympus ScanR (cube4)
book details
Widefield epifluorescence and transmission microscope, environment box 13-508B
Olympus ScanR (cube5)
book details
Widefield epifluorescence and transmission microscope, environment box, hardware autofocus 13-508B
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Special Objectives

Company Magnification and NA Immersion type
Leica
book
63x 1.2 (for SP5) water
Zeiss
book
40x 1.1 water
Zeiss
book
40x 1.2 water
Zeiss
book
63x 1.2 water
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Workstations

Equipment Information Room
ALMF VM Gold 01 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 8 CPU, 128 GB RAM, 8 GB vGPU, 100 GB temporary HDD
Software: Imaris

IT center
ALMF VM Gold 02 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 8 CPU, 128 GB RAM, 8 GB vGPU, 100 GB temporary HDD
Software:
Imaris

IT center
ALMF VM Gold 03 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 8 CPU, 128 GB RAM, 8 GB vGPU, 100 GB temporary HDD
Software:
Imaris

IT center
ALMF VM Silver 01 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 100 GB temporary HDD
Software:
Python

IT center
ALMF VM Silver 02 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 100 GB temporary HDD
Software:
Python

IT center
ALMF VM Silver 03 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 100 GB temporary HDD
Software:
Python

IT center

ALMF VM Silver 04 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 100 GB temporary HDD
Software:
Python

IT center
Workstation 1
book

Volocity, Amira, Zen, Cell Profiler, Fiji


13-501
Workstation 2 
book

Cell Profiler, Matlab, Fiji


13-501
Workstation 3 
book

Cell Profiler, Fiji, LAS AF, Nikon viewer


13-501

Additional Equipment

Equipment Features Room
Cell culture hood
book
For splitting cells and general cell culture work 13-505
Cell seeding device
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Automated cell seeding - 96 and 384 well plates 13-505
Integra 96/384 pipette
book
automated pipetting device for 96 and 384well plates
13-507
Liquidator (cell culture)
book
Manual 96 channel pipetting system - for cell staining / media change 13-505
Liquidator (lab)
book
Manual 96 channel pipetting system - for siRNA coating only 13-507
Speed Vac (MiVac A)
book
Vacuum concentrator for 96 and 384 well plates 13-507
Speed Vac (MiVac B)
book
Vacuum concentrator for 96 and 384 well plates 13-507
small heating chamber
book
small stage top environment chamber suitable for Maerzhaeuser stages. 13-502
Sutter needle puller
book
Device to prepare capillaries for microinjection 13-515B
Tecan plate washer
book
High-throughput plate washer 13-505

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Room Label Phone number
13-509 cherry 8608
13-514 orange 8646
13-515 banana 8647
13-516 plum 8649
13-501 visitor office 8629
     
overview building 13 floor 5
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