Remote support

We are offering remote support for most of our microscope systems. Please contact us, best by email or phone to get support. We will try our best to help you on demand if needed, but of course we can also schedule remote sessions.

Widefield Microscopes

Microscope Supported by Features Room
Nanolive 3D Cell Explorer Fluo
book details
Sabine Reither (almf@embl.de)
Beate Neumann (almf@embl.de)
3D Holo-Tomographic Live Cell Imaging Microscope
with Fluorescence Module DAPI-FITC-TRITC/Cy5 with stage top incubator
13-515D

Nikon Ti-2
book details

Sabine Reither (almf@embl.de)
Beate Neumann (almf@embl.de)

Motorized xy-stage, perfect focus system (PFS) hardware autofocus, LUMENCOR SPECTRA III, Penta filters in filter wheel EM: (432/36, 515/30, 596/30, 682/42, 809/89), Orca Fusion CMOS 13-508E
Nikon Ti-E
book details
Sabine Reither (almf@embl.de)
Beate Neumann (almf@embl.de)
Motorized xy-stage, perfect focus system (PFS) hardware autofocus, Lumencore spectraX, sCMOS PCO edge 4.2 CL 13-514A
Olympus Cell^R TIRF
book details
Marko Lampe (almf@embl.de)
Sabine Reither (almf@embl.de)
EMCCD, multi-color TIRF (405, 488, 561, 640 nm laser), Environment box (cellVivo), filterwheels, motorized xy-stage, hardware autofocus (ZDC3) 13-515E
Zeiss Cellobserver
book details
Sabine Reither (almf@embl.de)
Marko Lampe (almf@embl.de)
Environment box, metal halide fluorescence lamp, motorized xy-stage 13-515D
Zeiss MicroBeam
book details
Manuel Gunkel (almf@embl.de)
Stefan Terjung (almf@embl.de)
Laser microdissection microscope system, collection of samples by laser catapulting. 13-512
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Confocal Microscopes

Microscope Supported by Features Room
Leica SP8 STED 3X
book details

Marko Lampe (almf@embl.de)
Stefan Terjung (almf@embl.de)

Confocal laser scanning microscope with white-light laser (470-670 nm) and gated detection on two HyD detectors,
stimulated emission depletion (STED) 3D super-resolution (592, 660 and 775 nm STED lasers), environment box, motorized xy-stage
13-516B

Leica SP8 DLS
book details

Sabine Reither (almf@embl.de)
Stefan Terjung (almf@embl.de)

Confocal laser scanning microscope with light sheet option (SPIM). available laser wavelengths: 405, 488, 552, 640 nm, motorized xy-stage 13-509C
Nikon A1R HD25
book details
Lucas Schuetz (almf@embl.de)
Beate Neumann (almf@embl.de)
Confocal laser scanning microscope with galvano and resonant scanner and large field of view scanning also in resonant mode. Water immersion dispenser. Laser unit with 405, 488, 561 and 640 nm lasers. 13-5
Olympus FV1200
book details
Stefan Terjung (almf@embl.de)
Sabine Reither (almf@embl.de)
Confocal laser scanning microscope with two scanners, second scanner with pulsed lasers (355, 375, 532 nm) for nano-surgery and photo-uncagingFRAP, FRET, photo-activation, environment box, hardware autofocus (ZDC3) 13-516E
Olympus FV3000
book details
Stefan Terjung (almf@embl.de)
Aliaksander Halavatyi (almf@embl.de)
Confocal laser scanning microscope with 4 GaAsP spectral detectors, FRAP, FRET lasers: 405, 445, 488, 514, 561, 594, 640 nm, environment box, hardware autofocus (ZDC), motorized xy-stage 13-514E
Olympus iXplore SPIN SR
book details

Marko Lampe / CellSens (almf@embl.de)
Stefan Terjung / CellSens (almf@embl.de)
Beate Neumann / ScanR  (almf@embl.de)

Dual camera Spinning disk confocal microscope, Yokogawa CSU-W1 and SORA; lasers: 405, 445, 488, 514, 561, 640 nm, environment box, motorized xy-stage 13-515C
Zeiss LSM 780
book details


Manuel Gunkel (almf@embl.de)
Aliaksander Halavatyi (almf@embl.de)

Confocal laser scanning microscope with GaAsP spectral detector, FCS, FRAP, FRET lasers: 405, 458, 488, 514, 561, 633 nm, environment box, hardware autofocus (definite focus), motorized xy-stage 13-516C
Zeiss LSM 780 NLO
book details
Manuel Gunkel (almf@embl.de)
Aliaksander Halavatyi (almf@embl.de)
2-photon (can be used for nano-surgery and photo-uncaging) and confocal laser scanning microscope, FCS, FRAP, FRET,  GaAsP external detectors, spectral GaAsP detection, lasers: 405, 458, 488, 514, 561, 594, 633 nm, 690-1064 nm(2-photon laser), environment box, hardware autofocus (definite focus), motorized xy-stage 13-516D
Zeiss LSM 880 AIRY fast
book details
Manuel Gunkel (almf@embl.de)
Aliaksander Halavatyi (almf@embl.de)
Confocal laser scanning microscope with AIRY fast module. The AIRY fast module allows faster acquisition even with increased resolution compared to normal confocal scanning. 13-514B
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Lightsheet Microscopes

Microscope Supported by Features Room

Leica SP8 DLS
book details

Sabine Reither (almf@embl.de)
Stefan Terjung (almf@embl.de)

Confocal laser scanning microscope with light sheet option.  „Inverted“ light sheet microscope with two independent opposing light sheets and one detection objective. The system is equipped with an EMBL incubation box. Available magnifications and Laser lines are the following: 16x; 40x; 405nm; 488nm; 552nm; 638nm. The system is best for imaging thicker samples which can’t be mounted in a FEP tube or capillary and need environmental control.

13-509C
Luxendo MuVi SPIM
book details
Sabine Reither (almf@embl.de)
Stefan Terjung (almf@embl.de)
Light sheet microscope with parallel illumination from two sides and parallel detection from two sides perpendicular to the illumination. Possibility of sample rotation offers the opportunity to improve axial resolution and imaging quality after image. Available magnifications and Laser lines are the following: 33.3x; 22.2x;445nm; 488nm; 515nm; 561nm; 642nm. The system is best for imaging Drosophila, Zebrafish and thicker samples, which do not need CO2 control and can be mounted in a capillary or FEP tube. 13-509B
Luxendo InVi SPIM
book details
Sabine Reither (almf@embl.de)
Stefan Terjung (almf@embl.de)

„Inverted“ light sheet microscope with 2 cameras for simultaneous dual color acquisition. The system is equipped with temperature- and humidity control and a gas mixer to control CO2 and O2 levels. Available magnifications and Laser lines are the following: 31.25x; 62.5x; 445nm; 488nm; 515nm; 561nm; 594nm; 642nm. The system has one detection and one illumination objective and is best for small transparent samples, which are sensitive, difficult to mount and need environmental control.

13-509A
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Super-Resolution Microscopes

Microscope Supported by Features Room
Abberior RESOLFT
book details
Marko Lampe (almf@embl.de)
Stefan Terjung (almf@embl.de)
Combination of RESOLFT (super-resolution with switchable FPs) and STED (775 nm depletion laser) including DyMin STED option for improved resolution and decreased bleaching. Motorized xy/stage. 13-516A
Leica GSDIM
book details
Marko Lampe (almf@embl.de)
Stefan Terjung (almf@embl.de)
Localization based super-resolution in 3D by ground state depletion by individual return microscopy (GSDIM, also called dSTORM) 13-509D
Leica SP8 STED 3X
book details
Marko Lampe (almf@embl.de)
Stefan Terjung (almf@embl.de)
3D stimulated emission depletion (STED) microscopy with gated detection, environment box, motorized xy-stage, lasers: 405 nm, Ar-laser (458, 476, 488, 496, 514 nm), white-light laser (470-670 nm), STED laser (592, 660 + 775 nm) 13-516B
Zeiss Elyra 7 with Lattice SIM
book details

Marko Lampe (almf@embl.de)
Aliaksander Halavatyi (almf@embl.de)
Stefan Terjung (almf@embl.de)

The Elyra 7 is optimized for fast imaging with simultaneous dual camera (PCO Edge 4.2 sCMOS) acquisitions. It offers SMLM in TIRF, HiLo and Epi mode with optional 3D localization as well as Lattice SIM illumination. Motorized xy-stage, environment box. Lasers: 405, 488, 561, 642 nm 13-514C
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High-throughput microscopy

Microscope Supported by Features Room
Leica SP5 MSA (A)
book
 details
Aliaksander Halavatyi (almf@embl.de)
Sabine Reither (almf@embl.de)
Confocal laser scanning microscope, environment box, MSA, adaptive feedback microscopy, EMBL dispenser 13-508F
Molecular Devices IXM
book details
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)

Widefield epifluorescence and transmission microscope, sCMOS camera, Environment box, automated pipetting 13-508B
Nikon Ti-E PLateloader
book details
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)
Widefield epifluorescence and transmission microscope, hardware autofocus (PFS) and plateloader to autmatically load 96- and 384-well plates 13-508B
Olympus ScanR (cube4)
book details
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)
Widefield epifluorescence and transmission microscope, environment box 13-508B
Olympus ScanR (cube5)
book details
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)
Widefield epifluorescence and transmission microscope, environment box, hardware autofocus 13-508B
Zeiss LSM 900 AIRY
book details

Aliaksander Halavatyi (almf@embl.de)
Manuel Gunkel (almf@embl.de)

Confocal laser scanning microscope, AIRY scan, widefield camera, environment box, adaptive feedback microscopy 13-515A
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Special Objectives

Company Magnification and NA Immersion type
Leica
book
63x 1.2 (for SP5) water
Zeiss
book
40x 1.1 water
Zeiss
book
40x 1.2 water
Zeiss
book
63x 1.2 water
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Workstations

Equipment Information Room
ALMF VM Gold 01 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 8 Cores, 128 GB RAM, 8 GB vGPU, 100 GB temporary HDD
Software: Imaris common modules, Huygens, FIJI can be run from your network share

IT center
ALMF VM Gold 02 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 8 Cores, 128 GB RAM, 8 GB vGPU, 100 GB temporary HDD
Software:
Imaris common modules, Huygens, FIJI can be run from your network share

IT center
ALMF VM ZEN (EMBL 3D cloud)
book   details

Hardware: Windows-10, 8 Cores, 128 GB RAM, 8 GB vGPU, 100 GB temporary HDD
Software: Zeiss ZEN blue and black (mainly for AIRY data processing), Imaris including Batch+Cell+Vantage+Colloc+Filament+XT, Huygens, Matlab,FIJI can be run from your network share

IT center
ALMF VM Linux 01 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 8 Cores, 128 GB RAM, 8 GB vGPU, 100 GB temporary HDD
Software:
Linux

IT center
ALMF Remote WS 1
book   details

Hardware: Windows-10, 16 Cores, Nvidia Quadro RTX 6000 (24 GB GDDR6),768 GB RAM, temporary HDD
Software: Huygens Deconvolution and Multiview-Image registration, Imaris 3D visualization and analysis

IT center
ALMF VM Silver 01 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 100 GB temporary HDD
Software:
Python, Ilastik, FIJI can be run from your network share

IT center
ALMF VM Silver 02 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 100 GB temporary HDD
Software:
Python, Ilastik, FIJI can be run from your network share

IT center
ALMF VM Silver 03 (EMBL 3D cloud)
book   details

Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 100 GB temporary HDD
Software:
Python,Ilastik, FIJI can be run from your network share

IT center

Additional Equipment

Equipment Supported by Features Room
Cell culture hood
book
Sabine Reither (almf@embl.de)
Beate Neumann (almf@embl.de)
For splitting cells and general cell culture work 13-505
Cell seeding device
book
Sabine Reither (almf@embl.de)
Beate Neumann (almf@embl.de)
Automated cell seeding - 96 and 384 well plates 13-505
Integra 96/384 pipette
book
Sabine Reither (almf@embl.de)
Beate Neumann (almf@embl.de)
automated pipetting device for 96 and 384well plates
13-507
Liquidator (cell culture)
book
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)


Manual 96 channel pipetting system - for cell staining / media change 13-508A
PLATEMASTER (lab)
book
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)
Manual 96 channel pipetting system - for siRNA coating only 13-507
Speed Vac (MiVac A)
book
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)
Vacuum concentrator for 96 and 384 well plates 13-507
Speed Vac (MiVac B)
book
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)
Vacuum concentrator for 96 and 384 well plates 13-507
small heating chamber
book
Stefan Terjung (almf@embl.de) small stage top environment chamber suitable for Maerzhaeuser stages. 13-502
Tecan plate washer
book
Beate Neumann (almf@embl.de)
Sabine Reither (almf@embl.de)
High-throughput plate washer 13-508A

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Room Label Phone number
13-509 cherry 8608
13-514 orange 8646
13-515 banana 8647
13-516 plum 8649
13-501 visitor office 8629
     
overview building 13 floor 5
internal wiki