Widefield Microscopes

Microscope Features Room
Nikon Ti-E
book details
Motorized xy-stage, perfect focus system (PFS) hardware autofocus, Lumencore spectraX, emission filter wheel, sCMOS PCO edge 4.2 CL 13-514A
Olympus Cut^R
book details
Laser nanosurgery (355 nm with <1 ps pulse width controlled via Rapp Optoelctronic scanner), environment box, microinjection, motorized xy-stage 13-509E
Olympus Scan^R (CLEM)
book details
Motorized xy-stage, environment box, Cell^R (experiment manager) and Scan^R (high-throughput) software 13-514C
Olympus Cell^R TIRF
book details
EMCCD, multi-color TIRF (405, 488, 561, 640 nm laser), Environment box (cellVivo), filterwheels, motorized xy-stage, hardware autofocus (ZDC3) 13-515E
Olympus LV200
book details
bioluminescence and fluorescence detection, light shielding environment box, 13-515B
Zeiss Cellobserver
book details
Environment box, Lumen Dynamics 120LED light source, motorized xy-stage 13-515D
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Confocal Microscopes

Microscope Features Room
Leica SP8 STED 3X
book details
Confocal laser scanning microscope with white-light laser (470-670 nm) and gated detection on two HyD detectors,
stimulated emission depletion (STED) 3D super-resolution (592, 660 and 775 nm STED lasers), environment box, motorized xy-stage
13-516B

Leica SP8 DLS
book details

Confocal laser scanning microscope with light sheet option (SPIM). available laser wavelengths: 405, 488, 552, 640 nm, motorized xy-stage 13-509C
Olympus FV1200
book details
Confocal laser scanning microscope with two scanners, second scanner with pulsed lasers (355, 375, 532 nm) for nano-surgery and photo-uncagingFRAP, FRET, photo-activation, environment box, hardware autofocus (ZDC3) 13-516E
PE Ultraview ERS
book details
Spinning disk confocal microscope, FRET, lasers: 405, 488, 514, 561, 640 nm, environment box, motorized xy-stage
13-508D
PE Ultraview VoX
book details
Spinning disk confocal microscope, photokinesis unit (FRAP, photo-activation), FRET, environment box, lasers: 405, 440, 488, 514, 561, 640 nm, motorized xy-stage 13-514D
Zeiss LSM 780
book details
Confocal laser scanning microscope with GaAsP spectral detector, FCS, FRAP, FRET lasers: 405, 458, 488, 514, 561, 633 nm, environment box, hardware autofocus (definite focus), motorized xy-stage 13-516C
Zeiss LSM 780 NLO
book details
2-photon (can be used for nano-surgery and photo-uncaging) and confocal laser scanning microscope, FCS, FRAP, FRET,  GaAsP external detectors, spectral GaAsP detection, lasers: 405, 458, 488, 514, 561, 594, 633 nm, 690-1064 nm(2-photon laser), environment box, hardware autofocus (definite focus), motorized xy-stage 13-516D
Zeiss LSM 880 AIRY fast
book details
Confocal laser scanning microscope with AIRY fast module. The AIRY fast module allows faster acquisition even with increased resolution compared to normal confocal scanning. 13-514B
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Super-Resolution Microscopes

Microscope Features Room
Abberior RESOLFT
book details
Combination of RESOLFT (super-resolution with switchable FPs) and STED (775 nm depletion laser) microscope. Motorized xy/stage. 13-516A
Leica GSDIM
book details
Localization based super-resolution in 3D by ground state depletion by individual return microscopy (GSDIM, also called dSTORM) 13-509D
Leica SP8 STED 3X
book details
3D stimulated emission depletion (STED) microscopy with gated detection, environment box, motorized xy-stage, lasers: 405 nm, Ar-laser (458, 476, 488, 496, 514 nm), white-light laser (470-670 nm), STED laser (592, 660 + 775 nm) 13-516B
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High-throughput microscopy

Microscope Features Room

Acquifer IM03 High-throughput
book details

Widefield high-throughput imaging system, environment control. 13-515C

Leica SP5 MSA (A)
book
 details

Confocal laser scanning microscope, environment box, MSA, adaptive feedback microscopy, EMBL dispenser 13-508F

Leica SP5 MSA (B)
book
 details

Confocal laser scanning microscope, environment box, MSA, adaptive feedback microscopy, EMBL dispenser 13-508E
Molecular Devices IXM
book details
Widefield epifluorescence and transmission microscope, sCMOS camera, Environment box, automated pipetting 13-514E
Nikon Ti-E PLateloader
book details
Widefield epifluorescence and transmission microscope, hardware autofocus (PFS) and plateloader to autmatically load 96- and 384-well plates 13-508B
Olympus ScanR (cube4)
book details
Widefield epifluorescence and transmission microscope, environment box 13-508B
Olympus ScanR (cube5)
book details
Widefield epifluorescence and transmission microscope, environment box, hardware autofocus 13-508B
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Special Objectives

Company Magnification and NA Immersion type
Leica
book
63x 1.2 (for SP5) water
Zeiss
book
40x 1.1 water
Zeiss
book
40x 1.2 water
Zeiss
book
63x 1.2 water
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Workstations

Equipment Information Room
VM Horizon vm-almf-01
book

Hardware: CentOS-7, 8 CPU, 32 GB RAM, 2 GB vGPU, 1 TB HDD

Software: ImageJ

IT center
VM Horizon vm-almf-02
book

Hardware: Windows-10, 8 CPU, 128 GB RAM, 8 GB vGPU, 1 TB HDD
Software:
Imaris, ImageJ, CellProfiler

IT center
VM Horizon vm-almf-03
book

Hardware: Windows-10, 8 CPU, 64 GB RAM, 4 GB vGPU, 1 TB HDD
Software:
Imaris, ImageJ, CellProfiler

IT center
VM Horizon vm-almf-04
book
Hardware: Windows-10, 4 CPU, 32 GB RAM, 2 GB vGPU, 1 TB HDD
Software: Imaris, ImageJ, CellProfiler
IT center
Workstation 1
book

Volocity, Amira, Zen, Cell Profiler, Fiji


13-501
Workstation 2 
book

Imaris, Cell Profiler, Matlab, Fiji, Huygens Titan


13-501
Workstation 3 
book

Cell Profiler, Fiji, LAS AF, Nikon viewer


13-501

Additional Equipment

Equipment Features Room
Cell culture hood
book
For splitting cells and general cell culture work 13-505
Cell seeding device
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Automated cell seeding - 96 and 384 well plates 13-505
Integra 96/384 pipette
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automated pipetting device for 96 and 384well plates
13-507
Liquidator (cell culture)
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Manual 96 channel pipetting system - for cell staining / media change 13-505
Liquidator (lab)
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Manual 96 channel pipetting system - for siRNA coating only 13-507
Speed Vac (MiVac A)
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Vacuum concentrator for 96 and 384 well plates 13-507
Speed Vac (MiVac B)
book
Vacuum concentrator for 96 and 384 well plates 13-507
small heating chamber
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small stage top environment chamber suitable for Maerzhaeuser stages. 13-502
Sutter needle puller
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Device to prepare capillaries for microinjection 13-514C
Tecan plate washer
book
13-505

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Room Label Phone number
13-509 cherry 8608
13-514 orange 8646
13-515 banana 8647
13-516 plum 8649
13-501 visitor office 8629
     
overview building 13 floor 5
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