Electron Microscopes (EMCF)
These microscopes are maintained and managed by the EM core facility. We provide training and assistance to users who wish to use any of the equipment listed below.
| Microscope | Features | Room |
| Morgagni | screening microscope, equipped with a side-mounted 1K CCD Camera (SIS) | 318 |
| Biotwin CM120 Philips | microscope for screening and 2D acquisition, equipped with a bottom-mounted 1K CCD Camera (Keen View, SIS) | 305C |
| TECNAI F30, 300kV FEG, FEI | tomography microscope (plastic samples), and cryomicroscope, primarily for cellular tomography application | 322 |
| CrossBeam 540, Zeiss | Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging. | 319 |
Electron Microscopes (Structural Biology)
These microscopes are maintained and managed by EMBL's Structural and Computational Biology unit.
| Microscope | Features | Room |
| Tecnai T12, FEI | screening microscope for cryo samples, equipped with a bottom-mounted 4K CCD Camera (Gatan) | 316 |
| POLARA, 300kV FEG, FEI | cryo-microscope for high resolution molecular structure analysis, both single particle and tomography. Equipped with Energy Filter and 2K CCD camera plus a 4K pre-gif Direct Electron Detector (Falcon). | 320 |
| Titan Krios, 300kV FEG, FEI | Cryo-microscope for high-resolution structural analysis and tomography. Equipped with a direct electron detector, Energy Filter and automatic loading and refilling systems. | 312 |
Ultramicrotomes
For plastic sections
| Conventional Ultramicrotomes | Room | |
| UC7, Leica | plastic-1 | 325 |
| Ultracut S, Leica | plastic-2 | 325 |
| Ultracut T, Leica | plastic-3 | 323A |
For cryosections
| Cryo-ultra-microtomes | room #325 |
| UC6, Leica | cryo-3, for Tokuyasu cryosections |
| UC6, Leica | cryo-1, for frozen hydrated sections, located in a box with temperature and humidity control |
Sample preparation
High Pressure Freezer |
room #305A |
| EMPACT-2 (Leica) | cell cryofixation at a pressure of ~2050 bar, accepts copper carriers, equipped with rapid loader |
| HPM-010 (Abra-Fluid) | can accept larger samples, different types of carriers available |
Freeze Substitution Devices (FS) |
room #305A |
| AFS-2a, Leica |
process samples for manual freeze-substitution and plastic-embedding |
| AFS-2b, Leica |
process samples for manual freeze-substitution and plastic-embedding |
| AFS-2c, Leica |
process samples for manual freeze-substitution and plastic-embedding, acquired with the support of the HMLS |
Accessory equipment
1 Knife-maker, used to prepare glass knives for ultramicrotomy (Leica)
Location: Room 325
1 Freeze Substitution machine Leica AFS1
Carbon coaters
BALTEC BAE250
Leica EM ACE 600 acquired with the support of the HMLS
Location: Room 312
Glow discharge device
Location: Room 324