Electron Microscopes (EMCF)

These microscopes are maintained and managed by the EM core facility. We provide training and assistance to users who wish to use any of the equipment listed below.

Microscope Features Room
Morgagni screening microscope, equipped with a side-mounted 1K CCD Camera (SIS) 318
Biotwin CM120 Philips microscope for screening and 2D acquisition, equipped with a bottom-mounted 1K CCD Camera (Keen View, SIS) 305C
JEM 2100+, JEOL microscope for large-scale 2D screening and tomography. Flexible operation from 80-200kV. 317
TECNAI F30, 300kV FEG, FEI tomography microscope (plastic samples), and cryomicroscope, primarily for cellular tomography application 322
CrossBeam 550, Zeiss Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging. 311
CrossBeam 540, Zeiss Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging. 319
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Ultramicrotomes

For plastic sections

Conventional Ultramicrotomes Room
UC7, Leica plastic-1 325
UC7, Leica plastic-2 325
UC6, Leica plastic/cryo 327

For cryosections

Cryo-ultra-microtomes Room
UC6, Leica cryo-3, for Tokuyasu cryosections 325
UC6, Leica cryo-1, for frozen hydrated sections (and plastic) 327

Sample preparation

High Pressure Freezer

room #327
HPM-010 (Abra-Fluid) can accept larger samples, different types of carriers available

Freeze Substitution Devices (FS)

room #305A
AFS-2a, Leica
process samples for manual freeze-substitution and plastic-embedding
AFS-2b, Leica
process samples for manual freeze-substitution and plastic-embedding
AFS-2c, Leica
process samples for manual freeze-substitution and plastic-embedding, acquired with the support of the HMLS

Accessory equipment

1 Knife-maker, used to prepare glass knives for ultramicrotomy (Leica)
Location: Room 325

1 Freeze Substitution machine Leica AFS1

Carbon coater
Leica EM ACE 600 acquired with the support of the HMLS
Location: Room 312

Glow discharge device
Location: Room 324

Data analysis and exploration

Together with EMBL IT Services and other core facilities we develop solutions to share and explore large EM data.

In order to efficiently interact with large data without having to copy it from our central IT storage systems, EMBL has implemented virtual workstations for our users and staff.

Electron Microscopes (Structural Biology)

The cryo-electron microscopes are maintained and managed by EMBL's Structural and Computational Biology unit. Service for single-particle and tomography cryo-EM for structural biology projects is offered through the cryo-EM service platform.

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