Electron Microscopes (EMCF)
These microscopes are maintained and managed by the EM core facility. We provide training and assistance to users who wish to use any of the equipment listed below.
Microscope | Features | Room |
Morgagni | screening microscope, equipped with a side-mounted 1K CCD Camera (SIS) | 318 |
Biotwin CM120 Philips | microscope for screening and 2D acquisition, equipped with a bottom-mounted 1K CCD Camera (Keen View, SIS) | 305C |
JEM 2100+, JEOL | microscope for large-scale 2D screening and tomography. Flexible operation from 80-200kV. | 317 |
TECNAI F30, 300kV FEG, FEI | tomography microscope (plastic samples), and cryomicroscope, primarily for cellular tomography application | 322 |
CrossBeam 550, Zeiss | Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging. | 311 |
CrossBeam 540, Zeiss | Focused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging. | 319 |
Ultramicrotomes
For plastic sections
Conventional Ultramicrotomes | Room | |
UC7, Leica | plastic-1 | 325 |
UC7, Leica | plastic-2 | 325 |
UC6, Leica | plastic/cryo | 327 |
For cryosections
Cryo-ultra-microtomes | Room | |
UC6, Leica | cryo-3, for Tokuyasu cryosections | 325 |
UC6, Leica | cryo-1, for frozen hydrated sections (and plastic) | 327 |
Sample preparation
High Pressure Freezer |
room #327 |
HPM-010 (Abra-Fluid) | can accept larger samples, different types of carriers available |
Freeze Substitution Devices (FS) |
room #305A |
AFS-2a, Leica |
process samples for manual freeze-substitution and plastic-embedding |
AFS-2b, Leica |
process samples for manual freeze-substitution and plastic-embedding |
AFS-2c, Leica |
process samples for manual freeze-substitution and plastic-embedding, acquired with the support of the HMLS |
Accessory equipment
1 Knife-maker, used to prepare glass knives for ultramicrotomy (Leica)
Location: Room 325
1 Freeze Substitution machine Leica AFS1
Carbon coater
Leica EM ACE 600 acquired with the support of the HMLS
Location: Room 312
Glow discharge device
Location: Room 324
Data analysis and exploration
Together with EMBL IT Services and other core facilities we develop solutions to share and explore large EM data.
In order to efficiently interact with large data without having to copy it from our central IT storage systems, EMBL has implemented virtual workstations for our users and staff.
Electron Microscopes (Structural Biology)
The cryo-electron microscopes are maintained and managed by EMBL's Structural and Computational Biology unit. Service for single-particle and tomography cryo-EM for structural biology projects is offered through the cryo-EM service platform.