The Metabolomics Core Facility provides services for the analysis of metabolites and lipids.
The facility is equipped with a high-resolution mass spectrometry system (HRMS), triple quadrupole with an ion trap (QTRAP) mass spectrometer coupled with ultra-high performance liquid chromatography (UHPLC), and direct infusion system (NanoMate) for separation and detection of various classes of small molecules, metabolites, lipids, and drugs.
Major projects and accomplishments
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Identification and detection of various classes of small molecules, metabolites, and lipids in mammalian and microbial cells
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EMBL-MCF mass spectral library from over 700 metabolites
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Lipidomics profiling platform for comprehensive analysis of lipid species from multiple lipid classes (e.g. glycerols (TGs, DGs), phospholipids, free fatty acids, etc)
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Developed protocols to analyze over 100 metabolites in a targeted manner
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Relative quantitation of metabolites and lipids using internal standards
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Analysis of drugs and related metabolites
Services provided
We provide services for mass spectrometry-based analysis of small molecules, metabolites and lipids. We perform targeted and untargeted measurements of various classes of molecules ranging from small metabolites to lipids in a variety of biological matrices.
Further services
- Global untargeted metabolomics and lipidomics profiling
- Targeted metabolomics profiling
- Free fatty acid analysis
- Metabolite and lipid identification using MS and MS/MS analysis

- Drug and drug metabolite analysis
- Low-volume high-throughput direct infusion analysis (with Advion Nanomate)
Acknowledgment and Authorship:
As per EMBL Internal Publication Policy (No. 63); if the facility services were used in work described in a publication, the facility must be acknowledged. For acknowledgment, the following statement must be added:
“We acknowledge the support of the EMBL Metabolomics Core Facility (MCF) in the acquisition and analysis of mass spectrometry data”
In addition, If a staff member of the Metabolomics Core Facility contributes substantially to published work, the staff member should be included as an author in the published work.